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Volumn 258, Issue 3, 1998, Pages 941-947

Mutational analysis of chitin synthase 2 of Saccharomyces cerevisiae - Identification of additional amino acid residues involved in its catalytic activity

Author keywords

Catalytic site; Chitin synthase; Saccharomyces cerevisiae; Site directed mutagenesis

Indexed keywords

CHITIN SYNTHASE; FUNGAL PROTEIN; MUTANT PROTEIN;

EID: 0032535705     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2580941.x     Document Type: Article
Times cited : (37)

References (36)
  • 1
    • 0023052275 scopus 로고
    • The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo
    • Bulawa, C. E., Slater, M., Cabib, E., Au-Young, J., Sburlati, A., Adair, W. L. Jr & Robbins, P. W. (1986) The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo, Cell 46, 213-225.
    • (1986) Cell , vol.46 , pp. 213-225
    • Bulawa, C.E.1    Slater, M.2    Cabib, E.3    Au-Young, J.4    Sburlati, A.5    Adair W.L., Jr.6    Robbins, P.W.7
  • 2
    • 0024597893 scopus 로고
    • Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae
    • Cabib, E., Sburlati, A., Bowers, B. & Silverman, S. J. (1989) Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae, J. Cell Biol. 108, 1655-1672.
    • (1989) J. Cell Biol. , vol.108 , pp. 1655-1672
    • Cabib, E.1    Sburlati, A.2    Bowers, B.3    Silverman, S.J.4
  • 3
    • 0024041563 scopus 로고
    • Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae
    • Silverman, S. J., Sburlati, A., Slater, M. L. & Cabib, E. (1988) Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae, Proc. Natl Acad. Sci. USA 85, 4735-4739.
    • (1988) Proc. Natl Acad. Sci. USA , vol.85 , pp. 4735-4739
    • Silverman, S.J.1    Sburlati, A.2    Slater, M.L.3    Cabib, E.4
  • 4
    • 0025991799 scopus 로고
    • CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae
    • Valdivieso, M. H., Mol, P. C., Shaw, J. A., Cabib, E. & Duran, A. (1991) CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae, J. Cell Biol. 114, 101-109.
    • (1991) J. Cell Biol. , vol.114 , pp. 101-109
    • Valdivieso, M.H.1    Mol, P.C.2    Shaw, J.A.3    Cabib, E.4    Duran, A.5
  • 5
    • 0028897101 scopus 로고
    • Architecture of the yeast cell wall. The linkage between chitin and beta (1→3)-glucan
    • Kollar, R., Petrakova, E., Ashwell, G., Robbins, P. W. & Cabib, E. (1995) Architecture of the yeast cell wall. The linkage between chitin and beta (1→3)-glucan, J. Biol. Chem. 270, 1170-1178.
    • (1995) J. Biol. Chem. , vol.270 , pp. 1170-1178
    • Kollar, R.1    Petrakova, E.2    Ashwell, G.3    Robbins, P.W.4    Cabib, E.5
  • 7
    • 0029844403 scopus 로고    scopus 로고
    • Differential trafficking and timed localization of two chitin synthase proteins, Chs2p and Chs3p
    • Chuang, J. S. & Schekman, R. W. (1996) Differential trafficking and timed localization of two chitin synthase proteins, Chs2p and Chs3p, J. Cell Biol. 135, 597-610.
    • (1996) J. Cell Biol. , vol.135 , pp. 597-610
    • Chuang, J.S.1    Schekman, R.W.2
  • 8
    • 0029808478 scopus 로고    scopus 로고
    • Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway
    • Ziman, M., Chuang, J. S. & Schekman, R. W. (1996) Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway, Mol. Biol. Cell 7, 1909-1919.
    • (1996) Mol. Biol. Cell , vol.7 , pp. 1909-1919
    • Ziman, M.1    Chuang, J.S.2    Schekman, R.W.3
  • 9
    • 0030763146 scopus 로고    scopus 로고
    • A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall
    • DeMarini, D. J., Adams, A. E. M., Fares, H., Virgilio, C. D., Valle, G., Chuang, J. S. & Pringle, J. R. (1997) A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall, J. Cell Biol. 139, 75-93.
    • (1997) J. Cell Biol. , vol.139 , pp. 75-93
    • DeMarini, D.J.1    Adams, A.E.M.2    Fares, H.3    Virgilio, C.D.4    Valle, G.5    Chuang, J.S.6    Pringle, J.R.7
  • 10
    • 0031013414 scopus 로고    scopus 로고
    • Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p
    • Santos, B. & Snyder, M. (1997) Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p, J. Cell Biol. 136, 95-110.
    • (1997) J. Cell Biol. , vol.136 , pp. 95-110
    • Santos, B.1    Snyder, M.2
  • 11
    • 0028986927 scopus 로고
    • Multidomain architecture of beta-glycosyl transferases: Implications for mechanism of action
    • Saxena, I. M., Brown, R. M. Jr, Fevre, M., Geremia, R. A. & Henrissat, B. (1995) Multidomain architecture of beta-glycosyl transferases: implications for mechanism of action, J. Bacteriol. 177, 1419-1424.
    • (1995) J. Bacteriol. , vol.177 , pp. 1419-1424
    • Saxena, I.M.1    Brown R.M., Jr.2    Fevre, M.3    Geremia, R.A.4    Henrissat, B.5
  • 12
    • 0029072238 scopus 로고
    • Characterization of chitin synthase 2 of Saccharomyces cerevisiae: Implication of two highly conserved domains as pos-sible catalytic sites
    • Nagahashi, S., Sudoh, M., Ono, N., Sawada, R., Yamaguchi, E., Uchida, Y., Mio, T., Takagi, M., Arisawa, M. & Yamada-Okabe, H. (1995) Characterization of chitin synthase 2 of Saccharomyces cerevisiae: implication of two highly conserved domains as pos-sible catalytic sites, J. Biol. Chem. 270, 13961-13967.
    • (1995) J. Biol. Chem. , vol.270 , pp. 13961-13967
    • Nagahashi, S.1    Sudoh, M.2    Ono, N.3    Sawada, R.4    Yamaguchi, E.5    Uchida, Y.6    Mio, T.7    Takagi, M.8    Arisawa, M.9    Yamada-Okabe, H.10
  • 13
    • 0020529962 scopus 로고
    • Transformation of intact yeast cells treated with alkali cations
    • Ito, H., Fukuda, Y., Murata, K. & Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations, J. Bacteriol. 153, 163-168.
    • (1983) J. Bacteriol. , vol.153 , pp. 163-168
    • Ito, H.1    Fukuda, Y.2    Murata, K.3    Kimura, A.4
  • 14
    • 0028947904 scopus 로고
    • An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis
    • Hashimoto-Gotoh, T., Mizuno, T., Ogasahara, Y. & Nakagawa, M. (1995) An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis, Gene 152, 271-275.
    • (1995) Gene , vol.152 , pp. 271-275
    • Hashimoto-Gotoh, T.1    Mizuno, T.2    Ogasahara, Y.3    Nakagawa, M.4
  • 16
    • 0023026377 scopus 로고
    • Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae
    • Sburlati, A. & Cabib, E. (1986) Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae, J. Biol. Chem. 261, 15147-15152.
    • (1986) J. Biol. Chem. , vol.261 , pp. 15147-15152
    • Sburlati, A.1    Cabib, E.2
  • 17
    • 0028985614 scopus 로고
    • Synthesis of 'Nod'-like chitin oligosaccharides by the Xenopus developmental protein DG42
    • Semino, C. E. & Robbins, P. W. (1995) Synthesis of 'Nod'-like chitin oligosaccharides by the Xenopus developmental protein DG42, Proc. Natl Acad. Sci. USA 92, 3498-3501.
    • (1995) Proc. Natl Acad. Sci. USA , vol.92 , pp. 3498-3501
    • Semino, C.E.1    Robbins, P.W.2
  • 18
    • 0028355540 scopus 로고
    • The NodC protein of Azorhizobium caulinodans is an N-acetylglucosaminyltransferase
    • Geremia, R., Mergaert, P., Geelen, D., Montagu, M. & Holsters, M. (1994) The NodC protein of Azorhizobium caulinodans is an N-acetylglucosaminyltransferase, Proc. Natl Acad. Sci. USA 91, 2669-2673.
    • (1994) Proc. Natl Acad. Sci. USA , vol.91 , pp. 2669-2673
    • Geremia, R.1    Mergaert, P.2    Geelen, D.3    Montagu, M.4    Holsters, M.5
  • 19
    • 0021821329 scopus 로고
    • Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC
    • Jacobs, T. W., Egelhoff, T. T. & Long, S. R. (1985) Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC, J. Bacteriol. 162, 469-476.
    • (1985) J. Bacteriol. , vol.162 , pp. 469-476
    • Jacobs, T.W.1    Egelhoff, T.T.2    Long, S.R.3
  • 20
    • 0026918943 scopus 로고
    • The Rhizobium, Bradyrhizobium, and Azorhizobium NodC proteins are homologous to yeast chitin synthases
    • Debelle, F., Rosenberg, C. & Denarie, J. (1992) The Rhizobium, Bradyrhizobium, and Azorhizobium NodC proteins are homologous to yeast chitin synthases, Mol. Plant-Microbe Interact. 5, 443-446.
    • (1992) Mol. Plant-microbe Interact. , vol.5 , pp. 443-446
    • Debelle, F.1    Rosenberg, C.2    Denarie, J.3
  • 22
    • 0024757755 scopus 로고
    • Similar and different domains of chitin synthases 1 and 2 of S. cerevisiae: Two isozymes with distinct functions
    • Silverman, S. J. (1989) Similar and different domains of chitin synthases 1 and 2 of S. cerevisiae: two isozymes with distinct functions, Yeast 5, 459-467
    • (1989) Yeast , vol.5 , pp. 459-467
    • Silverman, S.J.1
  • 23
    • 0025058606 scopus 로고
    • Isolation of a chitin synthase gene (CHS1) from Candida albicans by expression in Saccharomyces cerevisiae
    • Au-Young, J. & Robbins, P. W. (1990) Isolation of a chitin synthase gene (CHS1) from Candida albicans by expression in Saccharomyces cerevisiae, Mol. Microbiol. 4, 197-207.
    • (1990) Mol. Microbiol. , vol.4 , pp. 197-207
    • Au-Young, J.1    Robbins, P.W.2
  • 26
    • 0027994068 scopus 로고
    • The Neurospora crassa chs-2 gene encodes a non-essential chitin synthase
    • Din, A. B. & Yarden, O. (1994) The Neurospora crassa chs-2 gene encodes a non-essential chitin synthase, Microbiology 140, 2189-2197.
    • (1994) Microbiology , vol.140 , pp. 2189-2197
    • Din, A.B.1    Yarden, O.2
  • 27
    • 0026275198 scopus 로고
    • Chitin synthase 1 plays a major role in cell wall biogenesis in Neurospora crassa
    • Yarden, O. & Yanofsky, C. (1991) Chitin synthase 1 plays a major role in cell wall biogenesis in Neurospora crassa, Genes Dev. 5, 2420-2430.
    • (1991) Genes Dev. , vol.5 , pp. 2420-2430
    • Yarden, O.1    Yanofsky, C.2
  • 28
    • 85008542964 scopus 로고
    • Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus
    • Motoyama, T., Sudoh, M., Horiuchi, H., Ohta, A. & Takagi, M. (1994b) Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus, Biosci. Biotechnol. Biochem. 58, 1685-1693.
    • (1994) Biosci. Biotechnol. Biochem. , vol.58 , pp. 1685-1693
    • Motoyama, T.1    Sudoh, M.2    Horiuchi, H.3    Ohta, A.4    Takagi, M.5
  • 29
    • 0030033740 scopus 로고    scopus 로고
    • The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis
    • Weiss, N., Sztejnberg, A. & Yarden, O. (1996) The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis, Gene 168, 99-102.
    • (1996) Gene , vol.168 , pp. 99-102
    • Weiss, N.1    Sztejnberg, A.2    Yarden, O.3
  • 30
    • 0028876674 scopus 로고
    • A multigene family related to chitin synthase genes of yeast in the opportunistic pathogen Aspergillus fumigatus
    • Mellado, E., Aufauvre-Brown, A., Specht, C. A., Robbins, P. W. & Holden, D. W. (1995) A multigene family related to chitin synthase genes of yeast in the opportunistic pathogen Aspergillus fumigatus, Mol. Gen. Genet. 246, 353-359.
    • (1995) Mol. Gen. Genet. , vol.246 , pp. 353-359
    • Mellado, E.1    Aufauvre-Brown, A.2    Specht, C.A.3    Robbins, P.W.4    Holden, D.W.5
  • 31
    • 0029925168 scopus 로고    scopus 로고
    • The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions
    • Mellado, E., Aufauvre-Brown, A., Gow, N. A. & Holden, D. W. (1996) The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions, Mol. Microbiol. 20, 667-679.
    • (1996) Mol. Microbiol. , vol.20 , pp. 667-679
    • Mellado, E.1    Aufauvre-Brown, A.2    Gow, N.A.3    Holden, D.W.4
  • 32
    • 0026519160 scopus 로고
    • CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: The CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis
    • Bulawa, C. E. (1992) CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis, Mol. Cell. Biol. 12, 1764-1776.
    • (1992) Mol. Cell. Biol. , vol.12 , pp. 1764-1776
    • Bulawa, C.E.1
  • 33
    • 0027422254 scopus 로고
    • Cloning of the chitin synthase 3 gene from Candida albicans and its expression during yeast-hyphal transition
    • Sudoh, M., Nagahashi, S., Doi, M., Ohta, A., Takagi, M. & Arisawa, M. (1993) Cloning of the chitin synthase 3 gene from Candida albicans and its expression during yeast-hyphal transition, Mol. Gen. Genet. 241, 351-358.
    • (1993) Mol. Gen. Genet. , vol.241 , pp. 351-358
    • Sudoh, M.1    Nagahashi, S.2    Doi, M.3    Ohta, A.4    Takagi, M.5    Arisawa, M.6
  • 34
    • 0030039795 scopus 로고    scopus 로고
    • chs-4, a class IV chitin synthase gene from Neurospora crassa
    • Din, A. B., Specht, C. A., Robbins, P. W. & Yarden, O. (1996) chs-4, a class IV chitin synthase gene from Neurospora crassa, Mol. Gen. Genet. 250, 214-222.
    • (1996) Mol. Gen. Genet. , vol.250 , pp. 214-222
    • Din, A.B.1    Specht, C.A.2    Robbins, P.W.3    Yarden, O.4
  • 36
    • 0031561120 scopus 로고    scopus 로고
    • A novel fungal gene encoding chitin synthase with a myosin motor-like domain
    • Fujiwara, M., Horiuchi, H., Ohta, A. & Takagi, M. (1997) A novel fungal gene encoding chitin synthase with a myosin motor-like domain, Biochem. Biophys. Res. Commun. 236, 75-78.
    • (1997) Biochem. Biophys. Res. Commun. , vol.236 , pp. 75-78
    • Fujiwara, M.1    Horiuchi, H.2    Ohta, A.3    Takagi, M.4


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