Stable, long-term bacterial production of soluble, dimeric, disulfide-bonded protein pharmaceuticals without antibiotic selection

Biotechnology Progress

Volumn 16, Issue 1, 2000, Pages 17-25

  Mantile, Giuditta ^a   Fuchs, Chana ^b   Cordella Miele, Eleonora ^a,c   Peri, Alessandro ^a,d   Mukherjee, Anil B ^a   Miele, Lucio ^a,c  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^c LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

^d UNIVERSITY OF FLORENCE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOPLASM; DISULFIDE BONDED HUMAN UTEROGLOBIN; ENTEROBACTERIA; NONSELECTIVE LIQUID MEDIUM;

CONTINUOUS CULTURE; ESCHERICHIA COLI; EXPRESSION VECTOR; PLASMID VECTOR; PROTEIN EXPRESSION; RECOMBINANT DNA TECHNOLOGY; RECOMBINANT PROTEIN; UTEROGLOBIN;

BIOCHEMISTRY; CELL CULTURE; CHEMICAL BONDS; DRUG PRODUCTS; ESCHERICHIA COLI; PROTEINS;

BIOTECHNOLOGY;

ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BIOPHARMACEUTICS; BIOTECHNOLOGY; DIMERIZATION; DISULFIDES; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HUMANS; OLIGONUCLEOTIDE PROBES; PLASMIDS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SOLUBILITY; UTEROGLOBIN;

BACTERIA (MICROORGANISMS); ENTEROBACTERIA; ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 0033952984     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990129g     Document Type: Article

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