Import, processing, and assembly of the α- and β-subunits of chloroplast pyruvate dehydrogenase

Planta

Volumn 211, Issue 1, 2000, Pages 72-76

  Johnston, Mark L ^a,c   Miernyk, Jan A ^a,b   Randall, Douglas D ^a  

^a University of Missouri   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c MONSANTO COMPANY   (United States)

Author keywords

Chloroplast; Pisum; Precursor; Processing; Transit peptide

Indexed keywords

ADENOSINE TRIPHOSPHATE; CHLOROPLAST; CHROMATOGRAPHY; COMPLEMENTARY DNA; ENZYME PRECURSOR; ENZYME SUBUNIT; ENZYME SYNTHESIS; GEL PERMEATION CHROMATOGRAPHY; MITOCHONDRION; PRECIPITATION; PROTEIN HYDROLYSIS; PYRUVATE DEHYDROGENASE; RECOMBINANT ENZYME;

PISUM; PISUM SATIVUM; SATIVUM;

EID: 0033948019     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004250000261     Document Type: Article

References (22)

1. Arnon DI (1949) Copper enzymes in isolated Chloroplasts. Polyphenol-oxidase in Beta vulgaris. Plant Physiol 24: 1-15
2. de Boer AD, Wiesbeek PJ (1991) Chloroplast protein topogenesis: import, sorting, and assembly. Biochim Biophys Acta 1071: 221-253
3. Bruce BD, Perry S, Froehlich J, Keegstra K (1994) In vitro import of proteins into chloroplasts. Plant Mol Biol Manual 11: 1-15
4. Camp PJ, Randall DD (1985) Purification and characterization of the pea chloroplast pyruvate dehydrogenase complex. Plant Physiol 77: 571-577
5. Emanuelsson O, Nielsen H, von Heijne G (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci 8: 978-984
6. Fang T-K, David NR, Miernyk JA, Randall DD (1987) Isolation and purification of functional pea leaf mitochondria free of chlorophyll contamination. Curr Top Plant Biochem Physiol 6: 175
7. Highfield PE, Ellis RJ (1978) Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271: 420-424
8. Johnston ML, Luethy MH, Miernyk JA, Randall DD (1997) Cloning and molecular analyses of the Arabidopsis thaliana plastid pyruvate dehydrogenase subunits. Biochim Biophys Acta 1321: 200-206
9. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685
10. Lübeck J, Heins L, Soll J (1997) Protein import into chloroplasts. Physiol Plant 100: 55-64
11. 1beta subunit of pyruvate dehydrogenase from Arabidopsis thaliana. Biochim Biophys Acta 1187: 95-98
12. 1alpha subunit. Gene 164: 251-254
13. Luethy MH, Miernyk JA, Randall DD (1996) Plant pyruvate dehydrogenase complexes. In: Patel MS, Roche TE, Harris RA, (eds) Alpha-keto acid dehydrogenase complexes. Birkhauser AG, Basel, pp 71-92
14. Mooney B, Miernyk JA, Randall DD (1999) Cloning and characterization of the dihydrolipoamide S-acetyltransferase (E2) subunit of the plastid pyruvate dehydrogenase complex from Arabidopsis thaliana. Plant Physiol 120: 443-452
15. Olsen LJ, Keegstra K. (1992) The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. J Biol Chem 267: 433-439
16. Ferryman RA, Mooney B, Harmey MA (1995) Identification of a 42-kDa plant mitochondrial outer membrane protein, MOM42, involved in the import of precursor proteins into plant mitochondria. Arch Biochem Biophys 316: 659-664
17. Randall DD, Miernyk JA (1990) The mitochondrial pyruvate dehydrogenase complex. In: Lea PJ (ed) Methods in plant biochemistry, vol 3: Enzymes of primary metabolism. Academic Press, London, pp 175-192
18. Randall DD, Miernyk JA, David NR, Gemel J, Luethy MH (1995) Regulation of leaf mitochondrial pyruvate dehydrogenase complex activity by reversible phosphorylation. Proc Phytochem Soc Europe 37: 87-103
19. Römisch J, Tropschug M, Sebald W, Weiss H (1987) The primary structure of cytochrome c1 from Neurospora crassa. Eur J Biochem 164: 111-115
20. Sjöling S, Glaser E (1998) Mitochondrial targeting peptides in plants. Trends Plant Sci 3: 136-140
21. Thelen JJ, Luethy MH, Miernyk JA, Randall DD (1998) Partial purification and regulation of the maize mitochondrial pyruvate dehydrogenase complex. Plant Physiol 116: 1443-1450
22. Towbin H, Staehelin T, Gordon T (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354