Protein import into chloroplasts

Physiologia Plantarum

Volumn 100, Issue 1, 1997, Pages 53-64

  Lübeck, Jens ^a   Heins, Lisa ^a   Soll, Jürgen ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

Chloroplasts; envelope membranes; import apparatus; protein transport; sorting; targeting

Indexed keywords

EID: 0345678951     PISSN: 00319317     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3054.1997.1000105.x     Document Type: Article

References (131)

1. Alefsen, H., Waegemann, K. & Soll, J. 1994. Analysis of the protein import machinery. - J. Plant Physiol. 144: 339-345.
2. America, T., Hageman, J., Guera, A., Rook, F., Archer, K., Keegstra, K. & Weisbeek, P. 1994. Methotrexate does not block import of a DHFR fusion protein into chloroplasts. -Plant Mol. Biol. 24: 283-294.
3. Arkowitz, R. A. & Bassilana, M. 1994. Protein translocation in Escherichla coli. - Biochim. Biophys. Acta 1197: 311-343.
4. Berghöfer, J., Karnauchov, I., Herrmann, R. G. & Klösgen, R. B. 1995. Isolation and characterization of a cDNA encoding the secA protein from spinach chloroplasts. Evidence for azide resistance of sec-dependent protein translocation across thylakoid membranes in spinach. - J. Biol. Chem. 270: 18341-18346.
5. Boutry, M., Nagy, F., Poulsen, C., Aoyagi, K. & Chua, N. H. 1987. Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants. - Nature 328: 340-342.
6. Brink, S., Flügge, U.-I., Chaumont, F., Boutry, M., Emmermann, M., Schmilz, U., Becker, K. & Pfanner, N. 1994. Preproteins of chloroplast envelope inner membrane contain targeting information for receptor-dependent import into fungal mitochondria. - J. Biol. Chem. 269: 16478-16485.
7. _ , Fischer, K., Klösgen, R.-B. & Flügge, U.-I. 1995. Sorting of nuclear encoded chloroplast membrane proteins to the envelope and the thylakoid membrane. - J. Biol. Chem. 270: 20808-20815.
8. Brodsky, J. L. 1996. Post-translational protein translocation: Not all hsc70s are created equal. - Trends Biochem. Sci. 21: 122-126.
9. Castro Silva Filho, M., Chaumont, F., Leterme, S. & Boutry, M. 1996. Mitochondrial and chloroplast targeting sequences in tandem modify protein import specificity in plant organelles. - Plant Mol. Biol. 30: 769-780.
10. Chua, N.-H. & Schmidt, G. W. 1978. Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. - Proc. Natl. Acad. Sci. USA 75: 6110-6114.
11. _ & Schmidt, G. W. 1979. Transport of proteins into mitochondria and chloroplasts.-J. Cell Biol. 81: 461-483.
12. Cline, K. & Henry, R. 1996. Import and routing of nucleus-encoded chloroplast proteins. - Annu. Rev. Cell Dev. Biol. 12: 1-26.
13. _ , Werner-Washburne, M., Andrews, J. & Keegstra, K. 1984. Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts. - Plant Physiol. 75: 675-678.
14. _ , Werner-Washburne, M., Lubben, T. H. & Keegstra, K. 1985. Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts. - J. Biol. Chem. 260: 3691-3696.
15. _ , Ettinger, W. F. & Theg, S. M. 1992. Protein-specific energy requirements for protein transport across or into thylakoid membranes. Two lumenal proteins are transported in the absence of ATP. - J. Biol. Chem. 267: 2688-2696.
16. Cornwell, K. L. & Keegstra, K. 1987. Evidence that a chloroplast surface protein is associated with a specific binding site for the precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. - Plant Physiol. 85: 780-785.
17. de Boer, A. D. & Weisbeek, P. J. 1991. Chloroplast protein topogenesis: Import, sorting and assembly. - Biochim. Biophys. Acta 1071: 221-253.
18. de Kruijff, B. 1994. Anionic phospholipids and protein translocation. - FEBS Lett. 346: 78-82.
19. Dreses-Werringloer, U., Fischer, K., Wachter, E., Link, T. A. & Flügge, U.-I. 1990. cDNA sequence and deduced amino acid sequence of the precursor of the 37 kDa inner envelope membrane polypeptide from spinach chloroplasts: Its transit peptide contains an amphiphilic α-helix as the only detectable structural element. - Eur. J. Biochem. 195: 361-368.
20. Eilers, M. & Schatz. G. 1986. Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. - Nature 322: 228-232.
21. Endo, T, Kawakami, M., Goto, A., America, T., Weisbeek, P. & Nakai, M. 1994. Chloroplast protein import. Chloroplast envelopes and thylakoids have different abilities to unfold proteins. - Eur. J. Biochem. 225: 403-409.
22. Fischer, K., Weber, A., Arbinger, B., Brink, S., Eckerskorn, C. & Flügge, U.-I. 1994. The 24 kDa outer envelope membrane protein from spinach chloroplasts: Molecular cloning, in vivo expression and import pathway of a protein with unusual properties. - Plant Mol. Biol. 25: 167-177.
23. Flachmann, R., Michalowski, C. B., Löffelhardt, W. & Bohnert, H. J. 1993. SecY, an integral subunit of the bacterial preprotein translocase, is encoded by a plastid genome. - J. Biol. Chem. 268:7514-7519.
24. Flügge, U.-I. & Hinz, G. 1986. Energy dependence of protein translocation into chloroplasts. - Eur. J. Biochem. 160: 563-570.
25. _ , Fischer, K., Gross, A., Sebald, W., Lottspeich, F. & Eckerskorn, C. 1989. The triose phosphate-3-phosphoglycerate-phosphate translocator of spinach chloroplasts: Nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts. -EMBO J. 8: 39-46.
26. _ , Weber, A., Fischer, K., Lottspeich, F., Eckerskorn, C., Waegemann, K. & Soll, J. 1991. The major chloroplast envelope polypeptide is the phosphate translocator and not the protein import receptor. - Nature 353: 364-367.
27. Franklin, A. E. & Huffman, N. E. 1993. Chloroplasts contain a homolog of the 54 kD subunit of the signal recognition particle. - J. Biol. Chem. 268: 22175-22180.
28. Friedman, A. L. & Keegstra, K. 1989. Chloroplast protein import. Quantitative analysis of precursor binding. - Plant Physiol. 89: 993-999.
29. Frydman, J., Nimmesgern, E., Ohtsuka, K. & Hartl, F. U. 1994. Folding of nascent polypeptide chains in a high molecular mass assembly with molecular chaperones. - Nature 370: 111-117.
30. Glick, B. S., Beasley, E. M. & Schatz, G. 1992. Protein sorting in mitochondria. - Trends Biochem. Sci. 17: 453-459.
31. Grossmann, A., Bartlett, S. & Chua, N.-H. 1980. Energy-dependent uptake of cytoplasmically synthesized polypeptides by chloroplasts. - Nature 285: 625-628.
32. 2 into mitochondria: Evidence for conservative sorting. - EMBO J. 14: 1349-1359.
33. Guéra, A., America, T., van Waas, M. & Weisbeek, P. 1993. A strong protein unfolding activity is associated with the binding of precursor chloroplast proteins to chloroplast envelopes. - Plant Mol. Biol. 23: 309-324.
34. Hachiya, N., Komiya, T., Alam, R., Iwahashi, J., Sakaguchi, M., Omura, T. & Mihara, K. 1994. MSF, a novel cytoplasmatic chaperone which functions in precursor targeting to mitochondria. - EMBO J. 13:5146-5154.
35. Hageman, J., Robinson, C., Smeekens, S. & Weisbeek, P. 1986. A thylakoid processing protease is required for complete maturation of the lumen protein plastocyanin. - Nature 324: 567-569.
36. _ , Baecke, C., Ebskamp, M., Pilon. R., Smeekens, S. & Weisbeek, P. 1990. Protein import into and sorting inside the chloroplast are independent processes. - Plant Cell 2: 479-494.
37. Hand, J. M., Szabo, L. J., Vasconcelos, A. C. & Cashmore, A. R. 1989. The transit peptide of a chloroplast thylakoid membrane protein is functionally equivalent to a stroma targeting sequence. - EMBO J. 8: 3195-3206.
38. Hartl, F.-U., Pfanner, N., Nicholson, D. W. & Neupert, W. 1989. Mitochondrial protein import. - Biochim. Biophys. Acta 988: 1-45.
39. _ , Hlodan, R. & Langer, T. 1994. Molecular chaperones in protein folding: The art to avoiding sticky situations. -Trends Biochem. Sci. 19: 20-25.
40. Hemmingsen, S. M., Woolford, C., van der Vries, S. M., Tilly, K., Dennis, D. T., Georgopoulos, C. P., Hendrix, R. W. & Ellis, R. J. 1988. Homologous plant and bacterial proteins chaperone oligomeric protein assembly. - Nature 333: 330-334.
41. Hirsch, S. & Soll, J. 1995. Import of a new chloroplast inner envelope protein is greatly stimulated by potassium phosphate. - Plant Mol. Biol. 27: 1173-1181.
42. _ , Muckel, E., Heemeyer, F., von Heijne, G. & Soll, J. 1994. A receptor component of the chloroplast protein translocation machinery. - Science 266: 1989-1992.
43. Hoffman, N. E. & Franklin, A. E. 1994. Evidence for a stromal GTP requirement for the integration of a chlorophyll albbinding polypeptide into thylakoid membranes. - Plant Physiol 105: 295-304.
44. Huang, J., Hack, E., Thornburg, W. & Myers, A. M. 1990. A yeast mitochondrial leader peptide functions in vivo as dual targeting signal for both chloroplasts and mitochondria. -Plant Cell 2: 1249-1260.
45. Huang, L., Adam, Z. & Hoffman, N. E. 1992. Deletion mutants of chlorophyll alb-binding proteins are efficiently imported into chloroplasts but do not integrate into thylakoid membranes. - Plant Physiol. 99: 247-255.
46. Hurt, E. C., Soltanifar, N., Goldschmidt-Clermont, M., Rochaix, J. D. & Schatz, G. 1986. The cleavable pre-sequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria. - EMBO J. 5: 1343-1350.
47. Joly, J. C. & Wickner, W. 1993. The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids. - EMBO J. 12: 255-263.
48. Kaderbhai, M. A., Pickering, T., Austen, B. M. & Kaderbhai, N. A. 1988. A photoactivatible synthetic transit peptide labels 30 kDa and 52 kDa polypeptides of the chloroplast inner envelope membrane. - FEBS Lett. 232: 313-316.
49. Keegstra, K., Olsen, L. J. & Theg, S. M. 1989. Chloroplastic precursors and their transport across the envelope membrane. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 40: 471-501.
50. Kerber, B. & Soll, J. 1992. Transfer of chloroplast-bound precursor protein into the translocation apparatus is impaired after phospholipase C treatment. - FEBS Lett. 306: 71-74.
51. Kessler, F. & Blobel, G. 1996. Interaction of the protein import and folding machineries in the chloroplast. - Proc. Natl. Acad. Sci. USA 93: 7684-7689.
52. _ , Blobel, G., Patel, H. A. & Schnell, D. J. 1994. Identification of two GTP-binding proteins in the chloroplast protein import machinery. - Science 266: 1035-1039.
53. Knight, J. S. & Gray, J. C. 1995. The N-terminal hydrophobic region of the mature phosphate translocator is sufficient for targeting to the chloroplast inner envelope membrane. -Plant Cell 7: 1421-1432.
54. Knott, T. G. & Robinson, C. 1994. The secA inhibitor, azide, reversibly blocks the translocation of a subset of proteins across the chloroplast thylakoid membrane. - J. Biol. Chem. 269: 7843-7846.
55. Ko, K., Bornemisza, O., Kourtz, L., Ko, Z. W., Plaxton, W. C. & Cashmore, A. R. 1992. Isolation and characterization of a cDNA clone encoding a cognate 70-kDa heat shock protein of the chloroplast envelope. - J. Biol. Chem. 267: 2986-2993.
56. _ , Budd, D., Wu, C., Seibert, F., Kourtz, L. & Ko, Z. W. 1995. Isolation and characterization of a cDNA clone encoding a member of the Com44/Cim44 envelope components of the chloroplast import apparatus. - J. Biol. Chem. 270: 28601-28608.
57. Laidler, V., Chaddock, A. M., Knott, T. G., Walker, D. & Robinson, C. 1995. A secY homolog in Arabidopsis thaliana. Sequence of a full-length cDNA clone and import of the precursor protein into chloroplasts. -J. Biol. Chem. 270: 17664-17667.
58. Lamppa, G. K. 1988. The chlorophyll alb-binding protein inserts into the thylakoids independent of its cognate transit peptide. - J. Biol. Chem. 263: 14996-14999.
59. Li, H.-M., Moore, T. & Keegstra, K. 1991. Targeting of proteins to the outer envelope membrane uses a different pathway than transport into chloroplasts. - Plant Cell 3: 709-717.
60. _ , Sullivan, T. D. & Keegstra, K. 1992. Information for targeting to the chloroplast inner envelope membrane is contained in the mature region of the maize Bt1-encoded protein. - J. Biol. Chem. 267: 18999-19004.
61. Li, X., Henry, R., Yuan. J., Cline, K. & Hoffman, N. E. 1995. A chloroplast homologue of the signal recognition particle subunit SPR54 is involved in the posttranslational integration of a protein into thylakoid membranes. - Proc. Natl. Acad. Sci. USA 92: 3789-3793.
62. Lithgow, T., Høj, P. B. & Hoogenraad, N. J. 1993. Do cytosolic factors prevent promiscuity at the membrane surface? -FEBS Lett. 329: 1-4.
63. Lubben, T. H., Donaldson, G. K., Viitanen, P. V. & Gatenby, A. A. 1989. Several proteins imported into chloroplasts form stable complexes with the groEL-related chloroplast molecular chaperone. - Plant Cell 1: 1223-1230.
64. Lübeck, J., Soll, J., Akita, M., Nielsen, E. & Keegstra, K. 1996. Topology of IEP110, a component of the chloroplastic protein import machinery present in the inner envelope membrane. - EMBO J. 15: 4230-4238.
65. Ma, Y., Kouranov, A., LaSala, S. E. & Schnell, D. J. 1996. Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope. - J. Cell Biol. 134: 315-327.
66. Madueño, F., Napier, J. A. & Gray, J. C. 1993. Newly imported Rieske iron-sulfur protein associates with both cpn60 and hsp70 in the chloroplast stroma. - Plant Cell 5: 1865-1876.
67. Mahlke, K., Pfanner, N., Martin, J., Horwich, A. L., Haitl, F.-U. & Neupert, W. 1990. Sorting pathways of mitochondrial inner membrane proteins. - Eur. J. Biochem. 192: 551-555.
68. Marshall, J. S., DeRocher, A. E., Keegstra, K. & Vierling, E. 1990. Identification of heat shock protein hsp70 homologues in chloroplasts. - Proc. Natl. Acad. Sci. USA 87: 374-378.
69. 0II is imported by a novel, third pathway. - EMBO J. 13: 1310-1317.
70. Moore, A. L., Wood, C. K. & Watts, F. Z. 1994. Protein import into plant mitochondria. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 545-575.
71. Muckel, E. & Soll, J, 1996. A protein import receptor of chloroplasts is inserted into the outer envelope membrane by a novel pathway. -J. Biol. Chem. 271: 23846-23852.
72. Murakami, K. & Mori, M. 1990. Purified presequence binding factor (PBF) forms an import-competent complex with a purified mitochondrial precursor protein. - EMBO J. 9: 3201-3208.
73. Nakai, M., Goto, A., Omata, T. & Endo, T. 1992. Cloning and characterization of the secY gene from the cyanobacterium Synechococcus PCC7942. - Biochim. Biophys. Acta 1171: 113-116.
74. _ , Nohara, T., Sugita, D. & Endo, T. 1994. Identification and characterization of the Sec-A protein homologue in the cyanobacterium Synechococcus PCC7942. - Biochem. Biophys. Res. Commun. 200: 844-851.
75. Nohara, T., Nakai, M., Goto, A. & Endo, T. 1995. Isolation and characterization of the cDNA for pea chloroplast secA. Evolutionary conservation of the bacterial-type secA-dependent protein transport within chloroplasts. - FEBS Lett. 364: 305-308.
76. Oblong, J. E. & Lamppa, G. K. 1992. Identification of two structurally related proteins involved in proteolytic processing of precursors targeted to the chloroplast. - EMBO J. 11: 4401-4409.
77. Olsen, L. J. & Keegstra, K. 1992. The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. - J. Biol. Chem. 267: 433-4-39.
78. _ , Theg, S. M., Selman, B. R. & Keegstra, K. 1989. ATP is required for the binding of precursor proteins to chloroplasts. - J. Biol. Chem. 264: 6724-6729.
79. Packer, J. C. L. & Howe, C. J. 1996. The cyanobacterial genome contains a single copy of the ffh gene encoding a homologue of the 54 kDa subunit of the signal recognition particle. -Plant Mol. Biol. 30: 659-665.
80. Pain, D., Kanwar, Y. S. & Blobel, G. 1988. Identification of a receptor for protein import into chloroplasts and its localization to envelope contact zones. - Nature 331: 232-237.
81. Perry, S. E. & Keegstra, K. 1994. Envelope membrane proteins that interact with chloroplastic precursor proteins. - Plant Cell 6: 93-105.
82. Pilon, M., de Boer, A. D., Knols, S. L., Koppelman, M. H., van der Graaf, R. M., de Kruijff, B. & Weisbeek, P. J. 1990. Expression in Escherichia coli and purification of a translocation-competent precursor of the chloroplast protein ferredoxin. - J. Biol. Chem. 265: 3358-3361.
83. _ , de Kruijff, B. & Weisbeek, P. J. 1992. New insights into the import mechanism of the ferredoxin precursor into chloroplasts. - J. Biol. Chem. 267: 2548-2556.
84. Rassow, J., Voos, W. & Pfanner, N. 1995. Partner proteins determine multiple function of Hsp70. - Trends Cell Biol. 5: 207-212.
85. Robinson, C. & Klösgen, R. B. 1994. Targeting of proteins into and across the thylakoid membrane - a multitude of mechanisms. - Plant Mol. Biol. 26: 15-24.
86. 0II integration. - Plant J. 10: 149-155.
87. 2 presequence are sorted to the mitochondrial intermembrane space independently of hsp60. - J. Biol. Chem. 269: 17279-17288.
88. Ryan, K. R. & Jensen, R. E. 1995. Protein translocation across mitochondrial membranes: What a long, strange trip it is. -Cell 83: 517-519.
89. Salomon, M., Fischer, K., Flügge, U.-I. & Soll, J. 1990. Sequence analysis and protein import studies of an outer chlo-roplast envelope polypeptide. - Proc. Natl. Acad. Sci. USA 87: 5778-5782.
90. Scaramuzzi, C. D., Hiller, R. G. & Stokes, H. W. 1992. Identification of a chloroplast encoded secA gene homologue in a chromophytic alga: Possible role in chloroplast protein translocation. - Curr. Genet. 22: 421-427.
91. Schatz, G. & Dobberstein, B. 1996. Common principles of protein translocation across membranes. - Science 271: 1519-1526.
92. Schindler, C., Hracky, R. & Soll, J. 1987. Protein transport into chloroplasts: ATP is a prerequisite. - Z. Naturforsch. 42c: 103-108.
93. Schnell, D. J. & Blobel, G. 1993. Identification of intermediates in the pathway of protein import into chloroplasts and their localization to envelope contact sites. - J. Cell Biol. 120: 103-115.
94. _ , Kessler, F. & Blobel, G. 1994. Isolation of components of the chloroplast protein import machinery. - Science 266: 1007-1012.
95. Schwanz, A. M. & Dayhoff, M. O. 1978. Origins of prokaryotes, eukaryotes, mitochondria and chloroplasts. - Science 199: 395-403.
96. Seedorf, M. & Soll, J. 1995. Copper chloride, an inhibitor of protein import into chloroplasts. - FEBS Lett. 367: 19-22.
97. _ , Waegemann, K. & Soll, J. 1995. A constituent of the chloroplast import complex represents a new type of GTP-binding protein. - Plant J. 7: 401-411.
98. Smeekens, S., Bauerle, C., Hageman, J., Keegstra, K. & Weisbeek, P. 1986. The role of the transit peptide in the routing of precursors toward different chloroplast compartments. -Cell 46: 365-375.
99. Smith, S. M. & Ellis, R. J. 1979. Processing of small subunit precursor of ribulose bisphosphate carboxylase and its assembly into whole enzyme are stromal events. - Nature 278: 662-664.
100. Soll, J. 1995. New insights into the protein import machinery of the chloroplast's outer envelope. - Bot. Acta 108: 277-282.
101. _ & Alefsen, H. 1994. The protein import apparatus of chloroplasts. - Physiol. Plant. 87: 433-440.
102. _ & Waegemann, K. 1992. A functionally active protein import complex from chloroplasts. - Plant J. 2: 253-256.
103. -, Fischer, I. & Keegstra, K. 1988. A guanosine 5′-triphosphate-dependent protein kinase is localized in the outer envelope membrane of pea chloroplasts. - Planta 176: 488-496.
104. _ , Berger, V. & Bennett, J. 1989. Adenylate effects on protein phosphorylation in the interenvelope lumen of pea chloroplasts. - Planta 177: 393-400.
105. _ , Alefsen, H., Böckler, B., Kerber, B., Salomon, M. & Waegemann, K. 1992. Comparison of two different translocation mechanisms into chloroplasts. - In Membrane Biogenesis and Protein Targeting (W. Neupert and R. Lill, eds), pp. 299-306. Elsevier, Amsterdam. ISBN 0-444-89638-4.
106. Sullivan, T. D., Strelow, L. I., Illingworth, C. A., Phillips, R. L. & Nelson, Q. E. 1991. Analysis of maize brittle-1 alleles and a defective suppressor-mutator-induced mutable allel. -Plant Cell 3: 1337-1348.
107. Sweet, D. J. & Gerace, L. 1995. Taking from the cytoplasm and giving to the pore: Soluble transport factors in nuclear protein import. - Trends Cell Biol. 5: 444-447.
108. Theg, S. M. & Scott, S. V. 1993. Protein import into chloroplasts. - Trends Cell Biol. 3: 186-190.
109. _ , Bauerle, C., Olsen, L. J., Selman, B. R. & Keegstra, K. 1989. Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes. - J. Biol. Chem. 264: 6730-6736.
110. Tranel, P. J., Froehlich, J., Goyal, A. & Keegstra, K. 1995. A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway. - EMBO J. 14: 2436-2446.
111. + reductase upon its import into chloroplasts. - FEBS Lett. 320: 198-202.
112. van der Vere, P. S., Bennet, T. M., Oblong, J. E. & Lamppa, G. K. 1995. A chloroplast processing enzyme involved in precursor maturation shares a zinc-binding motif with a recently recognized family of metalloendopeptidases. - Proc. Natl. Acad. Sci. USA 92: 7177-7181.
113. van Loon, A. P. G. M. & Schatz, G. 1987. Transport of proteins to the mitochondrial intermembrane space: The 'sorting domain' of the cytochrome c, presequence is a stop-transfer sequence for the mitochondrial inner membrane. - EMBO J. 6: 2441-2448.
114. van't Hof, R., Demel, R., Keegstra, K. & de Kruijff, B. 1991. Lipid-peptide interactions between fragments of the transit peptide of ribulose-1,5-bisphosphate carboxylase/oxygenase and chloroplast membrane lipids. - FEBS Lett. 291: 350-354.
115. _ , van Klompenburg, W., Pilon, M., Kozubek, A., de Korte-Kool, G., Demel, R., Weisbeek, P. J. & de Kruijff, B. 1993. The transit sequence mediates the specific interaction of the precursor of ferredoxin with chloroplast envelope membrane lipids. - J. Biol. Chem. 268: 4037-4042.
116. von Heijne, G. & Nishikawa, K. 1991. Hypothesis: Chloroplast transit peptides. The perfect random coil? - FEBS Lett. 278: 1-3.
117. _ , Steppuhn, J. & Herrmann, R. G. 1989. Domain structure of mitochondrial and chloroplast targeting peptides. - Eur. J. Biochem. 180: 535-545.
118. Waegemann, K. & Soll, J. 1991. Characterization ot the protein import apparatus in isolated outer envelopes of chloroplasts. -Plant J. 1: 149-158.
119. _ & Soll, J. 1993. Isolation and characterization of a functionally active protein translocation apparatus from chloroplast envelopes. - In Molecular Mechanisms of Membrane Traffic. NATO ASI Series, H74 (D. J. Morré, K. E. Howell and J. J. M. Gergerson, eds), pp. 101-104. Sprinser-Verlag, Berlin. ISBN 3-540-53096-7.
120. _ & Soll, J. 1996. Phosphorylation of the transit sequence of chloroplast precursor proteins. - J. Biol. Chem. 271: 6545-6554.
121. _ , Paulsen, H. & Soll, J. 1990. Translocation of proteins into isolated chloroplasts requires cytosolic factors to obtain import competence. - FEBS Lett. 261: 89-92.
122. _ , Eichacker, S. & Soll, J. 1992. Outer envelope membranes from chloroplasts ars isolated as right-side-out vesicles. -Planta 187: 89-94.
123. Walker. D., Chaddock, A. M., Chaddock, J. A., Roberts. L. M., Lord, J. M. & Robinson, C. 1996. Ricin A chain fused to a chloroplast-targeting signal is unfolded on the chloroplast surface prior to import across the envelope membranes. - J. Biol. Chem. 271: 4082-4085.
124. Weber, A., Menzlaff, E., Arbinger, B., Gutensohn, M., Eckerskorn, C. & Flügge, U.-I. 1995. The 2-oxoglutarate/malate translocator of chloroplast envelope membranes: Molecular cloning of a transporter containing a 12-helix motif and expression of the functional protein in yeast cells. - Biochemistry 34: 2621-2627.
125. Whelan, J., Knorpp, C. & Glaser, E. 1990. Sorting of precursor proteins between isolated spinach leaf mitochondria and chloroplasts. - Plant Mol. Biol. 14: 977-982.
126. _ , Knorpp, C., Harmey, M. A. & Glaser, E. 1991. Specificity of leaf mitochondrial and chloroplast processing systems for nuclear-encoded proteins. - Plant Mol. Biol. 16: 283-292.
127. Willey, D. L., Fischer, K., Wachter, E., Link, T. A. & Flügge, U.-I. 1991. Molecular cloning and structural analysis of the phosphate translocator from pea chloroplasts and its comparison to the spinach phosphate translocator. - Planta 183: 451-461.
128. Wu, C. & Ko, K. 1993. Identification of an uncleavable targeting signal in the 70-kilodalton spinach chloroplast outer envelope membrane protein. - J. Biol. Chem. 268: 19384-19391.
129. _ , Seibert, F. S. & Ko, K. 1994. Identification of chloroplast envelope proteins in close physical proximity to a partially translocated chimeric precursor protein. - J. Biol. Chem. 269: 32264-32271.
130. Yuan, J., Henry, R. & Cline, K. 1993. Stromal factors play an essential role in protein integration into thylakoids that cannot be replaced by unfolding or by heat shock protein hsp70. -Proc. Natl. Acad. Sci. USA 90: 8552-8556.
131. _ , Henry, R., McCaffery, M. & Cline, K. 1994. SecA homolog in protein transport within chloroplasts: Evidence for endosymbiont-derived sorting. - Science 266: 796-798.