Cloning and DNA sequence analysis of an immunogenic glucose-galactose MglB lipoprotein homologue from Brachyspira pilosicoli, the agent of colonic spirochetosis

Infection and Immunity

Volumn 68, Issue 8, 2000, Pages 4559-4565

  Zhang, P ^a   Cheng, X ^a   Duhamel, G E ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; GALACTOSE; GLUCOSE; LIPOPROTEIN;

ADOLESCENT; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL VIRULENCE; BRACHYSPIRA; COLON MUCOSA; CONTROLLED STUDY; DNA SEQUENCE; ENTERITIS; GENE REARRANGEMENT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SWINE; TREPONEMA;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, BACTERIAL; BASE SEQUENCE; CALCIUM-BINDING PROTEINS; CHICKENS; CLONING, MOLECULAR; COLONIC DISEASES; GALACTOSE; GENE DOSAGE; GLUCOSE; HUMANS; LIPOPROTEINS; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; PERIPLASMIC BINDING PROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SERPULINA; SPIROCHAETALES INFECTIONS; SWINE;

EID: 0033940569     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.8.4559-4565.2000     Document Type: Article

References (65)

1. Allweiss, B., J. Dostal, K. E. Carey, T. F. Edwards, and R. Freter. 1977. The role of chemotaxis in the ecology of bacterial pathogens of mucosal surfaces. Nature (London) 266:448-450.
2. Barcellos, D. E. S. N., M. R. Mathiesen, M. de Uzeda, I. I. T. A. Kader, and G. E. Duhamel. 2000. Prevalence of Brachyspira species isolated from diarrhoeic pigs in Brazil. Vet. Rec. 146:398-403.
3. Becker, P. S., D. R. Akins, J. D. Radolf, and M. V. Norgard. 1994. Similarity between the 38-kilodalton lipoprotein of Treponema pallidum and the glucose/galactose-binding (Mg1B) protein of Escherichia coli. Infect. Immun. 62:1381-1391.
4. Brandt, M. E., B. S. Riley, J. D. Radolf, and M. V. Norgard. 1990. Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infect. Immun. 58:983-991.
5. Cornfield, A. P., S. A. Wagner, J. R. Clamp, M. S. Kriaris, and L. C. Hoskins. 1992. Mucin degradation in the human colon: production of sialidase, sialate O-acetylesterase, N-acetylneuraminate lyase, arylesterase, and glycosulfatase activities by strains of fecal bacteria. Infect. Immun. 60:3971-3978.
6. Duhamel, G. E., N. Muniappa, M. R. Mathiesen, J. L. Johnson, J. Toth, R. O. Elder, and A. R. Doster. 1995. Certain canine weakly beta-hemolytic intestinal spirochetes are phenotypically and genotypically related to spirochetes associated with human and porcine intestinal spirochetosis. J. Clin. Microbiol. 33:2212-2215.
7. Duhamel, G. E., N. Muniappa, I. Gardner, M. A. Anderson, P. C. Blanchard, B. M. DeBey, M. R. Mathiesen, and R. L. Walker. 1995. Porcine colonic spirochetosis: a diarrheal disease associated with a newly recognized species of intestinal spirochaetes. Pig J. 35:101-110.
8. Duhamel, G. E. 1997. Intestinal spirochaetosis in non-production animals, p. 301-320. In D. J. Hampson and T. B. Stanton (ed.), Intestinal spirochaetosis in domestic animals and humans. CAB International, Wallingford, England.
9. Duhamel, G. E., R. O. Elder, N. Muniappa, M. R. Mathiesen, V. J. Wong, and R. P. Tarara. 1997. Colonic spirochetal infections of nonhuman primates associated with Brachyspira aalborgi, Serpulina pilosicoli, and unclassified flagellated bacteria. Clin. Infect. Dis. 25(Suppl. 2):186-188.
10. Duhamel, G. E., D. J. Trott, N. Muniappa, M. R. Mathiesen, K. Tarasiuk, J. I. Lee, and D. J. Hampson. 1998. Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated "Serpulina canis" sp. nov. J. Clin. Microbiol. 36:2264-2270.
11. Duhamel, G. E. 1998. Colonic spirochetosis caused by Serpulina pilosicoli. Large Anim. Pract. 19:14-22.
12. Elder, R. O., G. E. Duhamel, R. W. Schafer, M. R. Mathiesen, and M. Ramanathan. 1994. Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR. J. Clin. Microbiol. 32:1497-1502.
13. Fellström, C., B. Pettersson, J. Thomson, A. Gunnarsson, M. Persson, and K. Johansson. 1997. Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. J. Clin. Microbiol. 35:462-467.
14. Fisher, L. N., G. E. Duhamel, R. B. Westerman, and M. R. Mathiesen. 1997. Immunoblot reactivity of polyclonal and monoclonal antibodies with periplasmic flagellar proteins FlaA1 and FlaB of porcine Serpulina species. Clin. Diagn. Lab. Immunol. 4:400-404.
15. Fisher, L. N., M. R. Mathiesen, and G. E. Duhamel. 1997. Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species. Clin. Diagn. Lab. Immunol. 4:681-686.
16. Fraser, C. M., S. Casjens, W. M. Huang, G. G. Sutton, R. A. Clayton, R. Lathigra, O. White, K. A. Ketchum, R. Dodson, E. K. Hickey, M. Gwinn, B. Dougherty, J. F. Tomb, R. D. Fleischmann, D. Richardson, J. Peterson, A. R. Kerlavage, J. Quackenbush, S. Salzberg, M. Hanson, R. van Vugt, N. Palmer, M. D. Adams, J. D. Gocayne, J. Weidman, T. Utterback, L. Watthey, L. McDonald, P. Artiach, C. Bowman, S. Garland, C. Fujii, M. D. Cotton, K. Horst, K. Roberts, B. Hatch, H. O. Smith, and J. C. Venter. 1997. Genomic sequence of a Lyme disease spirochete, Borrelia burgdorferi. Nature 390:580-586.
17. Fraser, C. M., S. J. Norris, G. M. Weinstock, O. White, G. G. Sutton, R. Dodson, M. Gwinn, E. K. Hickey, R. Clayton, K. A. Ketchum, E. Sodergren, J. M. Hardham, M. P. McLeod, S. Salzberg, J. Peterson, H. Khalak, D. Richardson, J. K. Howell, M. Chidambaram, T. Utterback, L. McDonald, P. Artiach, C. Bowman, M. D. Cotton, C. Fujii, S. Garland, B. Hatch, K. Horst, K. Roberts, M. Sandusky, J. Weidman, H. O. Smith, and J. C. Venter. 1998. Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 281:375-388.
18. Harayama, S., J. Bollinger, T. Iino, and G. Hazelbauer. 1983. Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning. J. Bacteriol. 153:408-415.
19. Hayashi, S., and H. C. Wu. 1990. Lipoproteins in bacteria. J. Bioenerg. Biomembr. 22:451-471.
20. Hogg, R. W., C. Voelker, and I. V. Carlowitz. 1991. Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12. Mol Gen. Genet. 229:453-459.
21. Hugdahl, M. B., J. T. Beery, and M. P. Doyle. 1988. Chemotactic behavior of Campyobacter jejuni. Infect. Immun. 56:1560-1566.
22. Jones, M. J., J. M. Miller, and W. L. George. 1986. Microbiological and biochemical characterization of spirochetes isolated from the feces of homosexual males. J. Clin. Microbiol. 24:1071-1074.
23. Kalckar, H. M. 1976. The periplasmic galactose receptor protein of Escherichia coli in relation to galactose chemotaxis. Biochimie 58:81-85.
24. Kennedy, M. J., and R. J. Yancey. 1996. Motility and chemotaxis in Serpulina hyodysenteriae. Vet. Microbiol. 49:21-30.
25. - mutants of Serpulina hyodysenteriae: both flagellin subunits, FlaA and FlaB, are necessary for full motility and intestinal colonization. FEMS Microbiol. Lett. 153:119-128.
26. Lee, A., J. L. O'Rourke, P. J. Barrington, and T. J. Trust. 1986. Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse cecal model. Infect. Immun. 51:536-546.
27. Leser, T. D., K. Møller, T. K. Jensen, and S. E. Jorsal. 1997. Specific detection of Serpulina hyodysenteriae and potentially pathogenic weakly β-haemolytic porcine intestinal spirochetes by polymerase chain reaction targeting 23S rDNA. Mol. Cell. Probes 11:363-372.
28. Li, H., J. Ruby, N. Charon, and H. Kuramitsu. 1996. Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant. J. Bacteriol. 178:3664-3667.
29. Lin, E. C. C. 1996. Dissimilatory pathways for sugars, polyols, and carbohydrates, p. 307-342. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. American Society for Microbiology, Washington, D.C.
30. MacNab, R. M. 1987. Motility and chemotaxis, p. 732-759. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
31. Mantle, M., and C. Rombough. 1993. Growth in and breakdown of purified rabbit small intestinal mucin by Yersinia enterocolitica. Infect. Immun. 61: 4131-4138.
32. Marshall, T., and A. Allen. 1978. The isolation and characterization of the high-molecular-weight glycoprotein from pig colonic mucus. Biochem. J. 173:569-578.
33. Milner, J. A., and R. Sellwood. 1994. Chemotactic response to mucin by Serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization. Infect. Immun. 62:4095-4099.
34. Müller, N., H. Heine, and W. Boos. 1982. Cloning of mglB, the structural gene for the galactose-binding protein of Salmonella typhimurium and Escherichia coli. Mol. Gen. Genet. 185:473-480.
35. Muniappa, N., G. E. Duhamel, M. R. Mathiesen, and T. W. Bargar. 1996. Light microscopic and ultrastructural changes in the ceca of chicks inoculated with human and canine Serpulina pilosicoli. Vet. Pathol. 33:542-550.
36. Muniappa, N., M. R. Ramanathan, R. P. Tarara, R. B. Westerman, M. R. Mathiesen, and G. E. Dubamel. 1998. Attachment of human and rhesus Serpulina pilosicoli to cultured cells and comparison with a chick infection model. J. Spirochetal Tick-borne Dis. 5:44-53.
37. Neutra, M. R. 1980. Prokaryotic-eukaryotic cell junctions: attachment of spirochetes and flagellated bacteria to primate large intestinal cells. J. Ultrastruct. Res. 70:186-203.
38. Ochiai, S., Y. Adachi, and K. Mori. 1997. Unification of the genera Serpulina and Brachyspira, and proposals of Brachyspira hyodysenteriae comb. nov., Brachyspira innocens comb. nov. and Brachyspira pilosicoli comb. nov. Microbiol. Immunol. 41:445-452.
39. Oxberry, S. L., D. J. Trott, and D. J. Hampson. 1998. Serpulina pilosicoli, waterbirds and water: potential sources of infection for humans and other animals. Epidemiol. Infect. 121:219-225.
40. Porcella, S. F., T. G. Popova, K. E. Hagman, C. W. Penn, J. D. Radolf, and M. V. Norgard. 1996. A mgl-like operon in Treponema pallidum, the syphilis spirochete. Gene 177:115-121.
41. Quiocho, F. A., N. K. Vyas, and J. C. Spurlino. 1991. Atomic interactions between proteins and carbohydrates. Trans. Am. Crystallographic Assoc. 25:23-25.
42. Radolf, J. D., and M. V. Norgard. 1988. Pathogen specificity of Treponema pallidum subsp. pallidum integral membrane proteins identified by phase partitioning with Triton X-114. Infect. Immun. 56:1825-1828.
43. Radolf, J. D., M. V. Norgard, M. E. Brandt, R. D. Isaacs, P. A. Thompson, and B. Beutler. 1991. Lipoproteins of Borrelia burgdorferi and Treponema pallidum activate cachectin/tumor necrosis factor synthesis. J. Immunol. 147: 1968-1974.
44. Rosey, E. L., M. J. Kennedy, and R. J. Yancey, Jr. 1996. Dual flaA1 flaB1 mutant of Serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery. Infect. Immun. 64:4154-4162.
45. Ruane, P. J., M. M. Nakata, J. F. Reinhardt, and W. L. George. 1980. Spirochete-like organisms in the human gastrointestinal tract. Rev. Infect. Dis. 11:184-196.
46. Sacco, R. E., D. W. Trampel, and M. J. Wannemuehler. 1997. Experimental infection of C3H mice with avian, porcine, or human isolates of Serpulina pilosicoli. Infect. Immun. 65:5349-5353.
47. Sánchez, P. J., G. H. McCracken, Jr., G. D. Wendel, K. Olsen, N. Threlkeld, and M. V. Norgard. 1989. Molecular analysis of the fetal IgM response to Treponema pallidum antigens: implications for improved serodiagnosis of congenital syphilis. J. Infect. Dis. 159:508-517.
48. Sánchez, P. J., G. D. Wendel, Jr., E. Grimprel, M. Goldberg, M. Hall, O. Arencibia-Mireles, J. D. Radolf, and M. V. Norgard. 1993. Evaluation of molecular methodologies and rabbit infectivity testing for the diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum. J. Infect. Dis. 167:148-157.
49. Scholle, A., J. Vreemann, V. Blank, A. Nold, W. Boos, and M. D. Manson. 1987. Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors. Mol. Gen. Genet. 208:247-253.
50. Smith, A. C., and D. K. Podolsky. 1986. Colonic mucin glycoproteins in health and disease. Clin. Gastroenterol. 15:815-837.
51. Stanton, T. B., E. L. Rosey, M. J. Kennedy, N. S. Jensen, and B. T. Bosworth. 1999. Isolation, oxygen sensitivity, and virulence of NADH oxidase mutants of the anaerobic spirochete Brachyspira (Serpulina) hyodysenteriae, etiologic agent of swine dysentery. Appl. Environ. Microbiol. 65:5028-5034.
52. Taylor, D. J., J. R. Simmons, and H. M. Laird. 1980. Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae. Vet. Rec. 106:326-332.
53. Theisen, M., C. R. Rioux, and A. A. Potter. 1992. Molecular cloning, nucleotide sequence, and characterization of a 40,000-molecular-weight lipoprotein of Haemophilus somnus. Infect. Immun. 60:826-831.
54. Thomson, J. R., W. J. Smith, B. P. Murray, and S. McOrist. 1997. Pathogenicity of three strains of Serpulina pilosicoli in pigs with a naturally acquired intestinal flora. Infect. Immun. 65:3693-3700.
55. Thomson, J. R., W. J. Smith, and B. P. Murray. 1998. Investigations into field cases of porcine colitis with particular reference to infection with Serpulina pilosicoli. Vet. Rec. 142:235-239.
56. Trivett-Moore, N. L., G. L. Gilbert, C. L. H. Law, D. J. Trott, and D. J. Hampson. 1998. Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J. Clin. Microbiol. 36:261-265.
57. Trott, D. J., A. J. McLaren, and D. J. Hampson. 1995. Pathogenicity of human and porcine intestinal spirochetes in one-day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis. Infect. Immun. 63:3705-3710.
58. Trott, D. J., T. B. Stanton, N. S. Jensen, G. E. Duhamel, J. L. Johnson, and D. J. Hampson. 1996. Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int. J. Syst. Bacteriol. 46:206-215.
59. Trott, D. J., C. R. Huxtable, and D. J. Hampson. 1996. Experimental infection of newly weaned pigs with human and porcine strains of Serpulina pilosicoli. Infect. Immun. 64:4648-4654.
60. Trott, D. J., T. B. Stanton, N. S. Jensen, and D. J. Hampson. 1996. Phenotypic characteristics of Serpulina pilosicoli, the agent of intestinal spirochaetosis. FEMS Microbiol. Lett. 142:209-214.
61. Trott, D. J., B. G. Combs, A. S. J. Mikosza, S. L. Oxberry, I. D. Robertson, M. Passey, J. Taime, R. Sehuko, M. P. Alpers, and D. J. Hampson. 1997. The prevalence of Serpulina pilosicoli in humans and domestic animals in the eastern highlands of Papua New Guinea. Epidemiol. Infect. 119:369-379.
62. Trott, D. J., N. S. Jensen, I. Saint Girons, S. L. Oxberry, T. B. Stanton, D. Lindquist, and D. J. Hampson. 1997. Identification and characterization of Serpulina pilosicoli isolates recovered from the blood of critically ill patients. J. Clin. Microbiol. 35:482-485.
63. Vyas, N. K., M. N. Vyas, and F. A. Quiocho. 1988. Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein. Science 242:1290-1295.
64. Witters, N. A., and G. E. Duhamel. 1999. Motility-regulated mucin association of Serpulina pilosicoli, the agent of colonie spirochetosis of humans and animals. Adv. Exp. Med. Biol. 473:199-205.
65. Zhang, P., N. A. Witters, and G. E. Duhamel. 1999. Recovery from infection elicits serum IgG antibodies to specific Serpulina pilosicoli outer membrane antigens (SPOMA). Adv. Exp. Med. Biol. 473:191-197.