Identification of SopE2 from Salmonella typhimurium, a conserved guanine nucleotide exchange factor for Cdc42 of the host cell

Molecular Microbiology

Volumn 36, Issue 6, 2000, Pages 1206-1221

  Stender, Silke ^a   Friebel, Andrea ^a   Linder, Stefan ^b   Rohde, Manfred ^c   Mirold, Susanne ^a   Hardt, Wolf Dietrich ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b Inst für Kreislaufkrankheiten   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; PROTEIN CDC42;

ANIMAL CELL; ARTICLE; BACTERIAL VIRULENCE; CYTOSKELETON; GENETIC TRANSFECTION; HOST CELL; INTERNALIZATION; NONHUMAN; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM;

ACTIN-RELATED PROTEIN 2; ACTIN-RELATED PROTEIN 3; ACTINS; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; CDC42 GTP-BINDING PROTEIN; CELL SURFACE EXTENSIONS; COS CELLS; CYTOSKELETAL PROTEINS; CYTOSKELETON; ENDOCYTOSIS; GENES, BACTERIAL; GUANINE NUCLEOTIDE EXCHANGE FACTORS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; SALMONELLA TYPHIMURIUM;

EID: 0033923731     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01933.x     Document Type: Article

References (50)

1. Beltran, P., Plock, S.A., Smith, N.H., Whittam, T.S., Old, D.C., and Selander, R.K. (1991) Reference collection of strains of the Salmonella typhimurium complex from natural populations. J Gen Microbiol 137: 601-606.
2. Chen, L.M., Kaniga, K., and Galán, J.E. (1996a) Salmonella spp. are cytotoxic for cultured macrophages. Mol Microbiol 21: 1101-1115.
3. Chen, L.M., Hobbie, S., and Galán, J.E. (1996b) Requirement of CDC42 for Salmonella-induced cytoskeletal and nuclear responses. Science 274: 2115-2118.
4. Chen, L.M., Bagrodia, S., Cerione, R.A., and Galán, J.E. (1999) Requirement of p21-activated kinase (PAK) for Salmonella typhimurium-induced nuclear responses. J Exp Med 189: 1479-1488.
5. Cohen, P.T., and Cohen, P. (1989) Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221. Biochem J 260: 931-934.
6. Collazo, C.M., and Galán, J.E. (1996) Requirement for exported proteins isn secretion through the invasion-associated type III system of Salmonella typhimurium. Infect Immun 64: 3524-3531.
7. Collazo, C.M., and Galán, J.E. (1997) The invasion-associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Mol Microbiol 24: 747-756.
8. Collazo, C.M., Zierler, M.K., and Galán, J.E. (1995) Functional analysis of the Salmonella typhimurium invasion genes invl and invJ and identification of a target of the protein secretion apparatus encoded in the inv locus. Mol Microbiol 15: 25-38.
9. Donnenberg, M.S. (1999) Salmonella strikes a balance. Nature 401: 218-219.
10. Francis, C.L., Ryan, T.A., Jones, B.D., Smith, S.J., and Falkow, S. (1993) Ruffles induced by Salmonella and other stimuli direct macropinocytosis of bacteria. Nature 364: 639-642.
11. Fu, Y., and Galán, J.E. (1998) The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton. Mol Microbiol 27: 359-368.
12. Fu, Y., and Galán, J.E. (1999) A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion. Nature 401: 293-297.
13. Galán, J.E. (1998) Interactions of Salmonella with host cells: encounters of the closest kind. Proc Natl Acad Sci USA 95: 14006-14008.
14. Galán, J.E. (1999) Interaction of Salmonella with host cells through the centisome 63 type III secretion system. Curr Opin Microbiol 2: 46-50.
15. Galán, J.E., and Curtiss, R., III (1989) Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells. Proc Natl Acad Sci USA 86: 6383-6387.
16. Galán, J.E., Ginocchio, C., and Costeas, P. (1992) Molecular and functional characterization of the Salmonella invasion gene invA: homology of InvA to members of a new protein family. J Bacteriol 174: 4338-4349.
17. Galyov, E.E., Wood, M.W., Rosqvist, R., Mullan, P.B., Watson, P.R., Hedges, S., et al. (1997) A secreted effector protein of Salmonella dublin is translocated into eukaryotic cells and mediates inflammation and fluid secretion in infected ileal mucosa. Mol Microbiol 25: 903-912.
18. Ginocchio, C., Pace, J., and Galán, J.E. (1992) Identification and molecular characterization of a Salmonella typhimurium gene involved in triggering the internalization of salmonellae into cultured epithelial cells. Proc Natl Acad Sci USA 89: 5976-5980.
19. Guan, K.L., and Dixon, J.E. (1991) Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem 192: 262-267.
20. Gunn, J.S., Belden, W.J., and Miller, S.I. (1998) Identification of PhoP-PhoQ activated genes within a duplicated region of the Salmonella typhimurium chromosome. Microb Pathog 25: 77-90.
21. Hall, A. (1998) Rho GTPases and the actin cytoskeleton. Science 279: 509-514.
22. Hardt, W.-D., and Galán, J.E. (1997) A secreted Salmonella protein with homology to an avirulence determinant of plant pathogenic bacteria. Proc Natl Acad Sci USA 94: 9887-9892.
23. Hardt, W.-D., Chen, L.M., Schuebel, K.E., Bustelo, X.R., and Galán, J.E. (1998a) S. typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells. Cell 93: 815-826.
24. Hardt, W.-D., Urlaub, H., and Galán, J.E. (1998b) A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophage. Proc Natl Acad Sci USA 95: 2574-2579.
25. Hayward, R.D., and Koronakis, V. (1999) Direct nucleation and bundling of actin by the SipC protein of invasive Salmonella. EMBO J 18: 4926-4934.
26. Hersh, D., Monack, D.M., Smith, M.R., Ghori, N., Falkow, S., and Zychlinsky, A. (1999) The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1. Proc Natl Acad Sci USA 96: 2396-2401.
27. Hobbie, S., Chen, L.M., Davis, R.J., and Galán, J.E. (1997) Involvement of mitogen-activated protein kinase pathways in the nuclear responses and cytokine production induced by Salmonella typhimurium in cultured intestinal epithelial cells. J Immunol 159: 5550-5559.
28. Hoiseth, S.K., and Stocker, B.A. (1981) Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature 291: 238-239.
29. Hueck, C.J., Hantman, M.J., Bajaj, V., Johnston, C., Lee, C.A., and Miller, S.I. (1995) Salmonella typhimurium secreted invasion determinants are homologous to Shigella Ipa proteins. Mol Microbiol 18: 479-490.
30. Jones, M.A., Wood, M.W., Mullan, P.B., Watson, P.R., Wallis, T.S., and Galyov, E.E. (1998) Secreted effector proteins of Salmonella dublin act in concert to induce enteritis. Infect Immun 66: 5799-5804.
31. Kaniga, K., Bossio, J.C., and Galán, J.E. (1994) The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins. Mol Microbiol 13: 555-568.
32. Kaniga, K., Trollinger, D., and Galán, J.E. (1995a) Identification of two targets of the type III protein secretion system encoded by the inv and spa loci of Salmonella typhimurium that have homology to the Shigella IpaD and IpaA proteins. J Bacteriol 177: 7078-7085.
33. Kaniga, K., Tucker, S., Trollinger, D., and Galán, J.E. (1995b) Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella typhimurium entry into cultured epithelial cells. J Bacteriol 177: 3965-3971.
34. Machesky, L.M., and Gould, K.L. (1999) The Arp2/3 complex: a multifunctional actin organizer. Curr Opin Cell Biol 11: 117-121.
35. Miao, E.A., Scherer, C.A., Tsolis, R.M., Kingsley, R.A., Adams, L.G., Bäumler, A.J., et al. (1999) Salmonella typhimurium leucine-rich repeat proteins are targeted to the SPI1 and SPI2 type III secretion systems. Mol Microbiol 34: 850-864.
36. Miller, V.L., and Mekalanos, J.J. (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170: 2575-2583.
37. Mirold, S., Rabsch, W., Rohde, M., Stender, S., Tschape, H., Rüssmann, H., et al. (1999) Isolation of a temperate bacteriophage encoding the type III effector protein SopE from an epidemic Salmonella typhimurium strain. Proc Natl Acad Sci USA 96: 9845-9850.
38. Monack, D.M., Raupach, B., Hromockyi, A.E., and Falkow, S. (1996) Salmonella typhimurium invasion induces apoptosis in infected macrophages. Proc Natl Acad Sci USA 93: 9833-9838.
39. Norris, F.A., Wilson, M.P., Wallis, T.S., Galyov, E.E., and Majerus, P.W. (1998) SopB, a protein required for virulence of Salmonella dublin, is an inositol phosphate phosphatase. Proc Natl Acad Sci USA 95: 14057-14059.
40. Obert, S., O'Connor, R.J., Schmid, S., and Hearing, P. (1994) The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex. Mol Cell Biol 2: 1333-1346.
41. Rohatgi, R., Ma, L., Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., et al. (1999) The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell 97: 221-231.
42. Rudolph, M.G., Weise, C., Mirold, S., Hillenbrand, B., Bader, B., Wittinghofer, A., et al. (1999) Biochemical analysis of SopE from Salmonella typhimurium, a highly efficient guanosine nucleotide exchange factor for RhoGTPases. J Biol Chem 274: 30501-30509.
43. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
44. Tsolis, R.M., Adams, L.G., Ficht, T.A., and Bäumler, A.J. (1999) Contribution of Salmonella typhimurium virulence factors to diarrheal disease in calves. Infect Immun 67: 4879-4885.
45. Wang, R.F., and Kushner, S.R. (1991) Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100: 195-199.
46. Watson, P.R., Galyov, E.E., Paulin, S.M., Jones, P.W., and Wallis, T.S. (1998) Mutation of invH, but not stn, reduces Salmonella-induced enteritis in cattle. Infect Immun 66: 1432-1438.
47. Welch, M.D. (1999) The world according to arp: regulation of actin nucleation by the Arp2/3 complex. Trends Cell Biol 9: 423-427.
48. Wood, M.W., Rosqvist, R., Mullan, P.B., Edwards, M.H., and Galyov, E.E. (1996) SopE, a secreted protein of Salmonella dublin, is translocated into the target eukaryotic cell via a sip-dependent mechanism and promotes bacterial entry. Mol Microbiol 22: 327-338.
49. Zhou, D., Mooseker, M.S., and Galán, J.E. (1999a) Role of the S. typhimurium actin-binding protein SipA in bacterial internalization. Science 283: 2092-2095.
50. Zhou, D., Mooseker, M.S., and Galán, J.E. (1999b) An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proc Natl Acad Sci USA 96: 10176-10181.