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Volumn 274, Issue 5295, 1996, Pages 2115-2118

Requirement of CDC42 for Salmonella-induced cytoskeletal and nuclear responses

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CYTOSKELETON; ENZYME ACTIVATION; GENETIC ORGANIZATION; INTERNALIZATION; NONHUMAN; PATHOGENESIS; PINOCYTOSIS; PRIORITY JOURNAL; PROTEIN SECRETION; SALMONELLA; SIGNAL TRANSDUCTION;

EID: 0030448437     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5295.2115     Document Type: Article
Times cited : (269)

References (41)
  • 2
    • 0029879556 scopus 로고    scopus 로고
    • J. E. Galán, Curr. Topics Microbiol. Immunol. 209,43 (1995); J. E. Galán, Mol. Microbiol. 20, 263 (1996).
    • (1996) Mol. Microbiol. , vol.20 , pp. 263
    • Galán, J.E.1
  • 3
    • 0014037522 scopus 로고
    • A. Takeuchi, Am. J. Pathol. 50, 109 (1967); B. B. Finlay and S. Falkow, J. Infect. Dis. 162, 1096 (1990); C. Ginocchio, J. Pace, J. E. Galan, Proc. Natl. Acad. Sci. U.S.A. 89, 5976 (1992); C. L. Francis, T. A. Ryan, B. D. Jones, S. J. Smith, S. Falkow, Nature 364, 639 (1993).
    • (1967) Am. J. Pathol. , vol.50 , pp. 109
    • Takeuchi, A.1
  • 4
    • 0025156110 scopus 로고
    • A. Takeuchi, Am. J. Pathol. 50, 109 (1967); B. B. Finlay and S. Falkow, J. Infect. Dis. 162, 1096 (1990); C. Ginocchio, J. Pace, J. E. Galan, Proc. Natl. Acad. Sci. U.S.A. 89, 5976 (1992); C. L. Francis, T. A. Ryan, B. D. Jones, S. J. Smith, S. Falkow, Nature 364, 639 (1993).
    • (1990) J. Infect. Dis. , vol.162 , pp. 1096
    • Finlay, B.B.1    Falkow, S.2
  • 5
    • 0026778387 scopus 로고
    • A. Takeuchi, Am. J. Pathol. 50, 109 (1967); B. B. Finlay and S. Falkow, J. Infect. Dis. 162, 1096 (1990); C. Ginocchio, J. Pace, J. E. Galan, Proc. Natl. Acad. Sci. U.S.A. 89, 5976 (1992); C. L. Francis, T. A. Ryan, B. D. Jones, S. J. Smith, S. Falkow, Nature 364, 639 (1993).
    • (1992) Proc. Natl. Acad. Sci. U.S.A. , vol.89 , pp. 5976
    • Ginocchio, C.1    Pace, J.2    Galan, J.E.3
  • 6
    • 0027218124 scopus 로고
    • A. Takeuchi, Am. J. Pathol. 50, 109 (1967); B. B. Finlay and S. Falkow, J. Infect. Dis. 162, 1096 (1990); C. Ginocchio, J. Pace, J. E. Galan, Proc. Natl. Acad. Sci. U.S.A. 89, 5976 (1992); C. L. Francis, T. A. Ryan, B. D. Jones, S. J. Smith, S. Falkow, Nature 364, 639 (1993).
    • (1993) Nature , vol.364 , pp. 639
    • Francis, C.L.1    Ryan, T.A.2    Jones, B.D.3    Smith, S.J.4    Falkow, S.5
  • 8
    • 0028332527 scopus 로고
    • J. E. Galán, Trends Cell Biol. 4, 196 (1994); J. E. Galán, J. Pace, M. J. Hayman, Nature 357, 588 (1992); S. Ruschkowski, I. Rosenshine, B. B, Finlay. FFMS Microbiol. Lett. 74, 121 (1992).
    • (1994) Trends Cell Biol. , vol.4 , pp. 196
    • Galán, J.E.1
  • 9
    • 0026773530 scopus 로고
    • J. E. Galán, Trends Cell Biol. 4, 196 (1994); J. E. Galán, J. Pace, M. J. Hayman, Nature 357, 588 (1992); S. Ruschkowski, I. Rosenshine, B. B, Finlay. FFMS Microbiol. Lett. 74, 121 (1992).
    • (1992) Nature , vol.357 , pp. 588
    • Galán, J.E.1    Pace, J.2    Hayman, M.J.3
  • 12
    • 0028915743 scopus 로고
    • J. Chant and L. Stowers, ibid. 81, 1 (1995); A. B. Vojtek and J. A. Cooper, ibid. 62, 527 (1995); S. H. Zigmond, Curr. Opin. Cell Biol. 8, 66 (1996); C. D. Nobes and A. Hall, Cell 81 , 53 (1995).
    • (1995) Cell , vol.81 , pp. 1
    • Chant, J.1    Stowers, L.2
  • 13
    • 0029145073 scopus 로고
    • J. Chant and L. Stowers, ibid. 81, 1 (1995); A. B. Vojtek and J. A. Cooper, ibid. 62, 527 (1995); S. H. Zigmond, Curr. Opin. Cell Biol. 8, 66 (1996); C. D. Nobes and A. Hall, Cell 81 , 53 (1995).
    • (1995) Cell , vol.62 , pp. 527
    • Vojtek, A.B.1    Cooper, J.A.2
  • 14
    • 0030021649 scopus 로고    scopus 로고
    • J. Chant and L. Stowers, ibid. 81, 1 (1995); A. B. Vojtek and J. A. Cooper, ibid. 62, 527 (1995); S. H. Zigmond, Curr. Opin. Cell Biol. 8, 66 (1996); C. D. Nobes and A. Hall, Cell 81 , 53 (1995).
    • (1996) Curr. Opin. Cell Biol. , vol.8 , pp. 66
    • Zigmond, S.H.1
  • 15
    • 0028961293 scopus 로고
    • J. Chant and L. Stowers, ibid. 81, 1 (1995); A. B. Vojtek and J. A. Cooper, ibid. 62, 527 (1995); S. H. Zigmond, Curr. Opin. Cell Biol. 8, 66 (1996); C. D. Nobes and A. Hall, Cell 81 , 53 (1995).
    • (1995) Cell , vol.81 , pp. 53
    • Nobes, C.D.1    Hall, A.2
  • 16
    • 0024500613 scopus 로고
    • This system consists of an SV40 early gene promoter, which drives the expression of wild-type and mutant CDC42HS and Rac 1 ; this first cistron is followed by the internal ribosomal entry site of encephalomyocarditis virus [S. K. Jang, M. V. Davies, R. J. Kaufman, E. Wimmer, J. Virol. 63, 1651 (1989)] and the gene for a mutant form of GFP with increased fluorescence properties (GFPS65T) [R. Heim, A. B. Cubitt, R. Y. Tsien, Nature 373, 663 (1995)]. The two genes are transcribed as a single dicistronic mRNA and therefore both proteins are synthesized independently but in a coupled manner. The backbone of the expression vector was from pSG5 (Stratagene). The sources of the different forms of CDC42Hs and Rac1 were as follows: CDC42HsWT, CDC42HsN17, CDC42HsS188, and CDC42HsL61 were obtained as Bam HI-PVu II fragments of pGEX-2TKCDC42, pGEX-2TKCDC42N17, pZipneoCDC42S188, and pGEX-2TKCDC42L61, respectively [S. Bagrodia, B. Dérijard, R. J. Davis, R. A. Cerione, J. Biol. Chem. 270, 27995 (1995)]; Rad WT and Rac1V12 were obtained as 600 bp Eco RI fragments from pEXVRac1 and pEXVRac1Val12, respectively; and Rac1N17 was obtained by PCR from pGEX2TRac1 [A. J. Ridley, H. F. Paterson, C. L. Johnston, D. Diekmann, A. Hall, Cell 70, 401 (1992)].
    • (1989) J. Virol. , vol.63 , pp. 1651
    • Jang, S.K.1    Davies, M.V.2    Kaufman, R.J.3    Wimmer, E.4
  • 17
    • 0028921834 scopus 로고
    • This system consists of an SV40 early gene promoter, which drives the expression of wild-type and mutant CDC42HS and Rac 1 ; this first cistron is followed by the internal ribosomal entry site of encephalomyocarditis virus [S. K. Jang, M. V. Davies, R. J. Kaufman, E. Wimmer, J. Virol. 63, 1651 (1989)] and the gene for a mutant form of GFP with increased fluorescence properties (GFPS65T) [R. Heim, A. B. Cubitt, R. Y. Tsien, Nature 373, 663 (1995)]. The two genes are transcribed as a single dicistronic mRNA and therefore both proteins are synthesized independently but in a coupled manner. The backbone of the expression vector was from pSG5 (Stratagene). The sources of the different forms of CDC42Hs and Rac1 were as follows: CDC42HsWT, CDC42HsN17, CDC42HsS188, and CDC42HsL61 were obtained as Bam HI-PVu II fragments of pGEX-2TKCDC42, pGEX-2TKCDC42N17, pZipneoCDC42S188, and pGEX-2TKCDC42L61, respectively [S. Bagrodia, B. Dérijard, R. J. Davis, R. A. Cerione, J. Biol. Chem. 270, 27995 (1995)]; Rad WT and Rac1V12 were obtained as 600 bp Eco RI fragments from pEXVRac1 and pEXVRac1Val12, respectively; and Rac1N17 was obtained by PCR from pGEX2TRac1 [A. J. Ridley, H. F. Paterson, C. L. Johnston, D. Diekmann, A. Hall, Cell 70, 401 (1992)].
    • (1995) Nature , vol.373 , pp. 663
    • Heim, R.1    Cubitt, A.B.2    Tsien, R.Y.3
  • 18
    • 0028875683 scopus 로고
    • This system consists of an SV40 early gene promoter, which drives the expression of wild-type and mutant CDC42HS and Rac 1 ; this first cistron is followed by the internal ribosomal entry site of encephalomyocarditis virus [S. K. Jang, M. V. Davies, R. J. Kaufman, E. Wimmer, J. Virol. 63, 1651 (1989)] and the gene for a mutant form of GFP with increased fluorescence properties (GFPS65T) [R. Heim, A. B. Cubitt, R. Y. Tsien, Nature 373, 663 (1995)]. The two genes are transcribed as a single dicistronic mRNA and therefore both proteins are synthesized independently but in a coupled manner. The backbone of the expression vector was from pSG5 (Stratagene). The sources of the different forms of CDC42Hs and Rac1 were as follows: CDC42HsWT, CDC42HsN17, CDC42HsS188, and CDC42HsL61 were obtained as Bam HI-PVu II fragments of pGEX-2TKCDC42, pGEX-2TKCDC42N17, pZipneoCDC42S188, and pGEX-2TKCDC42L61, respectively [S. Bagrodia, B. Dérijard, R. J. Davis, R. A. Cerione, J. Biol. Chem. 270, 27995 (1995)]; Rad WT and Rac1V12 were obtained as 600 bp Eco RI fragments from pEXVRac1 and pEXVRac1Val12, respectively; and Rac1N17 was obtained by PCR from pGEX2TRac1 [A. J. Ridley, H. F. Paterson, C. L. Johnston, D. Diekmann, A. Hall, Cell 70, 401 (1992)].
    • (1995) J. Biol. Chem. , vol.270 , pp. 27995
    • Bagrodia, S.1    Dérijard, B.2    Davis, R.J.3    Cerione, R.A.4
  • 19
    • 0026654125 scopus 로고
    • This system consists of an SV40 early gene promoter, which drives the expression of wild-type and mutant CDC42HS and Rac 1 ; this first cistron is followed by the internal ribosomal entry site of encephalomyocarditis virus [S. K. Jang, M. V. Davies, R. J. Kaufman, E. Wimmer, J. Virol. 63, 1651 (1989)] and the gene for a mutant form of GFP with increased fluorescence properties (GFPS65T) [R. Heim, A. B. Cubitt, R. Y. Tsien, Nature 373, 663 (1995)]. The two genes are transcribed as a single dicistronic mRNA and therefore both proteins are synthesized independently but in a coupled manner. The backbone of the expression vector was from pSG5 (Stratagene). The sources of the different forms of CDC42Hs and Rac1 were as follows: CDC42HsWT, CDC42HsN17, CDC42HsS188, and CDC42HsL61 were obtained as Bam HI-PVu II fragments of pGEX-2TKCDC42, pGEX-2TKCDC42N17, pZipneoCDC42S188, and pGEX-2TKCDC42L61, respectively [S. Bagrodia, B. Dérijard, R. J. Davis, R. A. Cerione, J. Biol. Chem. 270, 27995 (1995)]; Rad WT and Rac1V12 were obtained as 600 bp Eco RI fragments from pEXVRac1 and pEXVRac1Val12, respectively; and Rac1N17 was obtained by PCR from pGEX2TRac1 [A. J. Ridley, H. F. Paterson, C. L. Johnston, D. Diekmann, A. Hall, Cell 70, 401 (1992)].
    • (1992) Cell , vol.70 , pp. 401
    • Ridley, A.J.1    Paterson, H.F.2    Johnston, C.L.3    Diekmann, D.4    Hall, A.5
  • 20
    • 0029818081 scopus 로고    scopus 로고
    • COS-1 cells were grown on glass cover slips in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal bovine serum (FBS) to 70% confluency and transfected with the plasmid vectors. Transfected cells were infected at an MOI of 50 for 1 hour at 37°C with wild-type S. typhimurium strain SL1344 that had been grown under conditions that stimulate the Type III protein secretion system [L. M. Chen, K. Kaniga, J. E. Galán, Mol. Microbiol. 21, 1101 (1996)]. Cells were usually infected 24 to 36 hours after transfection, when GFP could be visualized. Infected cells maintained at 37°C were examined on a Nikon Diaphot 300 inverted microscope.
    • (1996) Mol. Microbiol. , vol.21 , pp. 1101
    • Chen, L.M.1    Kaniga, K.2    Galán, J.E.3
  • 21
    • 12644276363 scopus 로고    scopus 로고
    • note
    • Transfected cells grown on glass cover slips were infected with strain SL1344 for 20 min at 37°C at an MOI of 50. Cells were washed, fixed, stained with rhodamine-labeled phalloidin, and examined under a fluorescence microscope.
  • 22
    • 0028085888 scopus 로고
    • Bacterial invasion was measured as in J. P. van Putten, J. F. L. Weel, and H. U. C. Grassmé [Methods Enzymol. 236, 420 (1994)] with minor modifications. Each cell invaded by Salmonella had an average of 31 ± 14 internalized bacteria.
    • (1994) Methods Enzymol. , vol.236 , pp. 420
    • Van Putten, J.P.1    Weel, J.F.L.2    Grassmé, H.U.C.3
  • 29
    • 0029015774 scopus 로고    scopus 로고
    • C. S. Hill, J. Wynne. R. Treisman, Cell 81, 1159 (1995); O. A. Coso et al, ibid., p. 1137.
    • Cell , pp. 1137
    • Coso, O.A.1
  • 30
    • 0028875683 scopus 로고
    • COS-1 cells grown in 6-cm culture dishes were transfected with 1 μg of pcDNA3-FLAG-Jnk-1, which encodes a FLAG epitope-tagged Jnk-1, and 2 μg of either pcDNA3CDC42HsN 17 or the vector pcDNAS. After 48, cells were mock-infected or infected with strain SL1344 for 30 min at an MOI of 20 in DMEM. The JNK kinase activity in cell lysates was determined as described previously [S. Bagrodia, B. Dérijard, R. J. Davis, R. A. Cerione, J. Biol. Chem. 270, 27995 (1995)].
    • (1995) J. Biol. Chem. , vol.270 , pp. 27995
    • Bagrodia, S.1    Dérijard, B.2    Davis, R.J.3    Cerione, R.A.4
  • 31
    • 0029063584 scopus 로고
    • M. P. Peppelenbosch et al., Cell 81, 849 (1995); M. P. Peppelenbosch, L G. J. Tertoolen, W. J. Hage, S. W. de Last, ibid. 74, 565 (1993); M. H. Tsai, C. L. Yu, D. W. Stacey, Science 250, 962 (1990); J. W. Han, F. McCormick, I. G. Macara, ibid. 252, 576 (1991); T.-H. Chuan, B. P. Bohl, G. M. Bokoch, J. Biol. Chem. 268, 26206 (1993).
    • (1995) Cell , vol.81 , pp. 849
    • Peppelenbosch, M.P.1
  • 32
    • 0027265532 scopus 로고
    • M. P. Peppelenbosch et al., Cell 81, 849 (1995); M. P. Peppelenbosch, L G. J. Tertoolen, W. J. Hage, S. W. de Last, ibid. 74, 565 (1993); M. H. Tsai, C. L. Yu, D. W. Stacey, Science 250, 962 (1990); J. W. Han, F. McCormick, I. G. Macara, ibid. 252, 576 (1991); T.-H. Chuan, B. P. Bohl, G. M. Bokoch, J. Biol. Chem. 268, 26206 (1993).
    • (1993) Cell , vol.74 , pp. 565
    • Peppelenbosch, M.P.1    Tertoolen, L.G.J.2    Hage, W.J.3    De Last, S.W.4
  • 33
    • 0029063584 scopus 로고
    • M. P. Peppelenbosch et al., Cell 81, 849 (1995); M. P. Peppelenbosch, L G. J. Tertoolen, W. J. Hage, S. W. de Last, ibid. 74, 565 (1993); M. H. Tsai, C. L. Yu, D. W. Stacey, Science 250, 962 (1990); J. W. Han, F. McCormick, I. G. Macara, ibid. 252, 576 (1991); T.-H. Chuan, B. P. Bohl, G. M. Bokoch, J. Biol. Chem. 268, 26206 (1993).
    • (1990) Science , vol.250 , pp. 962
    • Tsai, M.H.1    Yu, C.L.2    Stacey, D.W.3
  • 34
    • 0025811679 scopus 로고
    • M. P. Peppelenbosch et al., Cell 81, 849 (1995); M. P. Peppelenbosch, L G. J. Tertoolen, W. J. Hage, S. W. de Last, ibid. 74, 565 (1993); M. H. Tsai, C. L. Yu, D. W. Stacey, Science 250, 962 (1990); J. W. Han, F. McCormick, I. G. Macara, ibid. 252, 576 (1991); T.-H. Chuan, B. P. Bohl, G. M. Bokoch, J. Biol. Chem. 268, 26206 (1993).
    • (1991) Science , vol.252 , pp. 576
    • Han, J.W.1    McCormick, F.2    Macara, I.G.3
  • 35
    • 0027442667 scopus 로고
    • M. P. Peppelenbosch et al., Cell 81, 849 (1995); M. P. Peppelenbosch, L G. J. Tertoolen, W. J. Hage, S. W. de Last, ibid. 74, 565 (1993); M. H. Tsai, C. L. Yu, D. W. Stacey, Science 250, 962 (1990); J. W. Han, F. McCormick, I. G. Macara, ibid. 252, 576 (1991); T.-H. Chuan, B. P. Bohl, G. M. Bokoch, J. Biol. Chem. 268, 26206 (1993).
    • (1993) J. Biol. Chem. , vol.268 , pp. 26206
    • Chuan, T.-H.1    Bohl, B.P.2    Bokoch, G.M.3
  • 37
    • 0028078824 scopus 로고
    • E. Manser, T. Leung, H. Salihuddin, Z. Zhao, L. Lim, Nature 367, 40 (1994); G. A. Martin, G. Bollag, F. McCormick, A. Abo, EMBO J. 14, 77 (1995); M. Symons et al., Cell 84, 723 (1996); Y. Zheng, S. Bagrodia, R. A. Cerione, J. Biol. Chem. 269, 18727 (1994).
    • (1994) Nature , vol.367 , pp. 40
    • Manser, E.1    Leung, T.2    Salihuddin, H.3    Zhao, Z.4    Lim, L.5
  • 38
    • 0028078824 scopus 로고
    • E. Manser, T. Leung, H. Salihuddin, Z. Zhao, L. Lim, Nature 367, 40 (1994); G. A. Martin, G. Bollag, F. McCormick, A. Abo, EMBO J. 14, 77 (1995); M. Symons et al., Cell 84, 723 (1996); Y. Zheng, S. Bagrodia, R. A. Cerione, J. Biol. Chem. 269, 18727 (1994).
    • (1995) EMBO J. , vol.14 , pp. 77
    • Martin, G.A.1    Bollag, G.2    McCormick, F.3    Abo, A.4
  • 39
    • 0030006284 scopus 로고    scopus 로고
    • E. Manser, T. Leung, H. Salihuddin, Z. Zhao, L. Lim, Nature 367, 40 (1994); G. A. Martin, G. Bollag, F. McCormick, A. Abo, EMBO J. 14, 77 (1995); M. Symons et al., Cell 84, 723 (1996); Y. Zheng, S. Bagrodia, R. A. Cerione, J. Biol. Chem. 269, 18727 (1994).
    • (1996) Cell , vol.84 , pp. 723
    • Symons, M.1
  • 40
    • 0028338545 scopus 로고
    • E. Manser, T. Leung, H. Salihuddin, Z. Zhao, L. Lim, Nature 367, 40 (1994); G. A. Martin, G. Bollag, F. McCormick, A. Abo, EMBO J. 14, 77 (1995); M. Symons et al., Cell 84, 723 (1996); Y. Zheng, S. Bagrodia, R. A. Cerione, J. Biol. Chem. 269, 18727 (1994).
    • (1994) J. Biol. Chem. , vol.269 , pp. 18727
    • Zheng, Y.1    Bagrodia, S.2    Cerione, R.A.3
  • 41
    • 12644255666 scopus 로고    scopus 로고
    • note
    • We thank A, Abo, R. Cerione, R. Davis, and A. Hall for plasmids and cell lines, J. Lipsick for useful discussion, and D. Bar-Sagi for critical review of this manuscript. Supported by NIH grant GM52543 to J.E.G., who is an investigator of the American Heart Association.


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