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Thompson J.N. Specific hypotheses on the geographic mosaic of coevolution. Am Natur. 153:1999;S1-S14. This paper introduces a special issue of American Naturalist that is devoted to coevolutionary ecological studies in structured populations, including antagonistic and mutualistic interactions in plants and animals. The issue is a good diving board into empirical and theoretical coevolutionary ecology. Thompson continues to refine his theory of coevolution with geographical variation.
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The sequence analysis of linked but physically distant R-gene clusters shows that they diverge more than those within Cf-4/9, and that occasional recombination can bring these more diverged sequences together in the same R gene. Separate analyses of nonsynonymous and synonymous polymorphisms will lead to additional insights into the influence of positive selection on R genes, and might lead to statistics that can be used to parameterize models of the gene birth-death process
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Parniske M., Jones J.D.G. Recombination between diverged clusters of the tomato Cf-9 plant disease resistance gene family. Proc Natl Acad Sci USA. 96:1999;5850-5855. The sequence analysis of linked but physically distant R-gene clusters shows that they diverge more than those within Cf-4/9, and that occasional recombination can bring these more diverged sequences together in the same R gene. Separate analyses of nonsynonymous and synonymous polymorphisms will lead to additional insights into the influence of positive selection on R genes, and might lead to statistics that can be used to parameterize models of the gene birth-death process.
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Over 100 Kb of RPP5 sequence and comparison with the genome project RPP5 locus sequences reveal two totally different complex locus structures. The R genes in both haplotypes are transcribed and could encode functional products. Numerous short segments of shared polymorphisms provide evidence for gene conversion, whereas closer relationships between collinear R genes near the ends of the cluster as opposed to in the middle may result from crossing over. The authors recognize polymorphism maintenance in addition to diversifying selection on R genes
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Noel L., Moores T.L., van der Biezen E.A., Parniske M., Daniels M.J., Parker J.E., Jones J.D.G. Pronounced intraspecific haplotype divergence at the RPP5 complex disease resistance locus of Arabidopsis. Plant Cell. 11:1999;2099-2111. Over 100 Kb of RPP5 sequence and comparison with the genome project RPP5 locus sequences reveal two totally different complex locus structures. The R genes in both haplotypes are transcribed and could encode functional products. Numerous short segments of shared polymorphisms provide evidence for gene conversion, whereas closer relationships between collinear R genes near the ends of the cluster as opposed to in the middle may result from crossing over. The authors recognize polymorphism maintenance in addition to diversifying selection on R genes.
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Noel, L.1
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Sequences containing motifs common to most R genes, amplified by degenerate PCR and gathered from Genbank, reveal ancient relationships at the protein level and suggest that disease resistance may be a recruited function from a larger family of signaling genes. Searches against the Arabidopsis genome databases lead to an total estimate of some 200 nucleotide-binding site (NBS)-containing genes, many of which are found in clusters
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Meyers B.C., Dickerman A.W., Michelmore R.W., Sivaramakrishnan S., Sobral B.W., Young N.D. Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J. 20:1999;317-332. Sequences containing motifs common to most R genes, amplified by degenerate PCR and gathered from Genbank, reveal ancient relationships at the protein level and suggest that disease resistance may be a recruited function from a larger family of signaling genes. Searches against the Arabidopsis genome databases lead to an total estimate of some 200 nucleotide-binding site (NBS)-containing genes, many of which are found in clusters.
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Identification of regions in alleles of the flax rust resistance gene L that determine differences in gene-for-gene specificity
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Transgenic experiments prove that L allelic sequence differences are responsible for resistance specificity differences. Experiments using R-gene hybrid constructs show that in some cases LRR hypervariable segments confer resistance specificity, and in others a combination of LRR and other amino-terminal sequences act together. Differences outside the coding region or in the genetic background are also important
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Ellis J.G., Lawrence G.J., Luck J.E., Dodds P.N. Identification of regions in alleles of the flax rust resistance gene L that determine differences in gene-for-gene specificity. Plant Cell. 11:1999;495-506. Transgenic experiments prove that L allelic sequence differences are responsible for resistance specificity differences. Experiments using R-gene hybrid constructs show that in some cases LRR hypervariable segments confer resistance specificity, and in others a combination of LRR and other amino-terminal sequences act together. Differences outside the coding region or in the genetic background are also important.
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Silent sequence variation flanking Rpm1 allowed estimation of the ages of R-gene alleles. These ages have implications for coevolutionary outcomes in the information race. The Rpm1 polymorphism has been maintained for about ten million years. The data conform to the population genetic predictions of an epidemiological model with R-gene allele frequency cycling. The authors propose a 'trench warfare' hypothesis, in which disease severity and resistance allele frequencies cycle in plant-pathogen interactions
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Stahl E.A., Dwyer G., Mauricio R., Kreitman M., Bergelson J. Dynamics of disease resistance polymorphism at the RPM1 locus of Arabidopsis. Nature. 400:1999;667-671. Silent sequence variation flanking Rpm1 allowed estimation of the ages of R-gene alleles. These ages have implications for coevolutionary outcomes in the information race. The Rpm1 polymorphism has been maintained for about ten million years. The data conform to the population genetic predictions of an epidemiological model with R-gene allele frequency cycling. The authors propose a 'trench warfare' hypothesis, in which disease severity and resistance allele frequencies cycle in plant-pathogen interactions.
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A new Ac-like transposon of Arabidopsis is associated with a deletion of the RPS5 disease resistance gene
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Insertion deletion polymorphism for the entire Rps5 gene results from double-strand-break repair that may have involved a transposon rather than unequal crossing over with nearby Rfl1. The authors comment that the cost of resistance may contribute to resistance locus polymorphism, and put a ceiling on the total number of R genes in the genome
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Henk A.D., Warren R.F., Innes R.W. A new Ac-like transposon of Arabidopsis is associated with a deletion of the RPS5 disease resistance gene. Genetics. 151:1999;1581-1589. Insertion deletion polymorphism for the entire Rps5 gene results from double-strand-break repair that may have involved a transposon rather than unequal crossing over with nearby Rfl1. The authors comment that the cost of resistance may contribute to resistance locus polymorphism, and put a ceiling on the total number of R genes in the genome.
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Cloning of the single R-gene locus Bs2 opens the door for the evolutionary study of both sides of a gene-for-gene interaction. AvrBs2 confers a fitness benefit for Xanthomonas, implying that Bs2 resistance will be durable, and some strains carry AvrBs2 alleles that evade recognition
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Tai T.H., Dahlbeck D., Clark E.T., Gajiwala P., Pasion R., Whalen M.C., Stall R.E., Staskawicz B.J. Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato. Proc Natl Acad Sci USA. 96:1999;14153-14158. Cloning of the single R-gene locus Bs2 opens the door for the evolutionary study of both sides of a gene-for-gene interaction. AvrBs2 confers a fitness benefit for Xanthomonas, implying that Bs2 resistance will be durable, and some strains carry AvrBs2 alleles that evade recognition.
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Proc Natl Acad Sci USA
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Nanomolar concentrations of a peptide motif in flagellin elicit PR responses in a number of plant species. This peptide is highly conserved in diverse bacteria that are pathogenic to plants and animals. As most bacteria seem unable to delete this peptide, flagellin sensitivity may provide durable resistance
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Felix G., Duran J., Volko S., Boller T. Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J. 18:1999;265-276. Nanomolar concentrations of a peptide motif in flagellin elicit PR responses in a number of plant species. This peptide is highly conserved in diverse bacteria that are pathogenic to plants and animals. As most bacteria seem unable to delete this peptide, flagellin sensitivity may provide durable resistance.
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Felix, G.1
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Amazingly, Arabidopsis ecotypes display polymorphism for flagellin sensitivity (basically resistance phenotypes). Resistance segregates 3:1 and maps near the MRC-J resistance cluster, suggesting that Fls-1 is an R gene. The slow development of elicitor-treated seedlings and Fls-1 polymorphism suggest a high cost of sensitivity
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Gomez-Gomez L., Felix G., Boller T. A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana. Plant J. 18:1999;277-284. Amazingly, Arabidopsis ecotypes display polymorphism for flagellin sensitivity (basically resistance phenotypes). Resistance segregates 3:1 and maps near the MRC-J resistance cluster, suggesting that Fls-1 is an R gene. The slow development of elicitor-treated seedlings and Fls-1 polymorphism suggest a high cost of sensitivity.
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Meyers, B.C.1
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Variation at a microsatellite and two PCR-RFLP markers in the Dm3 locus correlates strongly with resistance phenotypes in lettuce cultivars. The variation shows both within-population diversity and between-population differentiation in wild relatives from California and Israel. Further characterization of Dm3 locus diversity and its correlation with marker diversity in the wild relatives, and of neutral marker variation, will identify the coevolutionary forces acting on Dm3 resistance
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Sicard D., Woo S.S., Arroyo-Garcia R., Ochoa O., Nguyen D., Korol A., Nevo E., Michelmore R. Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp. Theor Appl Genet. 99:1999;405-418. Variation at a microsatellite and two PCR-RFLP markers in the Dm3 locus correlates strongly with resistance phenotypes in lettuce cultivars. The variation shows both within-population diversity and between-population differentiation in wild relatives from California and Israel. Further characterization of Dm3 locus diversity and its correlation with marker diversity in the wild relatives, and of neutral marker variation, will identify the coevolutionary forces acting on Dm3 resistance.
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