Chlamydomonas reinhardtii: Biological rationale for genomics

Journal of Eukaryotic Microbiology

Volumn 47, Issue 4, 2000, Pages 340-349

  Dutcher, Susan K ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Basal bodies; Cytokinesis; Flagella; Microtubules

Indexed keywords

BIOLOGICAL FUNCTIONS; PLANT GENOME; PLANT MODEL;

ALGAE; ANIMALIA; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; EMBRYOPHYTA;

EID: 0033895219     PISSN: 10665234     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2000.tb00059.x     Document Type: Conference Paper

References (103)

1. Adams, G. M. W., Huang, B.-P. & Luck, D. J. L. 1983. Temperature-sensitive assembly-defective flagella mutants of Chlamydomonas reinhardtii. Genetics, 100:579-586.
2. Adams, G. M. W., Huang, B., Piperno, G. & Luck, D. J. L. 1981. Central pair-microtubular complex of Chlamydomonas flagella: polypeptide composition as revealed by analysis of mutants. J. Cell Biol., 91:69-76.
3. Afzelius, B. A. 1980. Genetic aspects of the immotile cilia syndrome. Clin. Genet., 17:52.
4. Aselson, C. M. & Lefebvre, P. A. 1998. Genetic analysis of flagellar length control in Chlamydomonas reinhardtii: a new long-flagella locus and extragenic suppressor mutations. Genetics, 148:693-702.
5. Barsel, S. E., Wexler, D. E. & Lefebvre, R A. 1988. Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics, 118:637-648.
6. Bolduc, C., Lee, V. D. & Huang, B. 1988. Beta-tubulin mutants of the unicellular green alga Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA, 85:131-135.
7. Casey, D. M.. Pazour, G. J., Wilkerson, C. G., Inaba, K., Koutoulis, A., Takada, S., Kamiya, R. & Witman, G. B. 1998. Identification and insertional mutagenesis of a new Chlamydomonas reinhardtii gene, oda14, that encodes the 25-kd subunit of the outer dynein arm docking complex. Mol. Biol. Cell 9:155a.
8. Cerruti, H., Johnson, A. M., Gillham, N. W. & Boynton, J. E. 1997. A eubacterial gene conferring spectinomycin resitance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics, 145:97-110.
9. Chen, J., Knowles, H. J., Hebert, J. L. & Hackett, B. P. 1998. Mutations of the mouse hepatocyte nuclear factor/Forkhead homologue4 gene results in an absence of cilia and random left-right symmetry. J. Clin. invest., 102:1077-1082.
10. Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C. & Rosenbaum, J. L. 1998. Chlamydomonas kinesin II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons. J. Cell Biol., 141:993-1008.
11. Curry, A. M., Williams, B. D. & Rosenbaum, J. L. 1992. Sequence analysis reveals homology between two proteins of the flagellar radial spoke. Mol. Cell. Biol., 12:3967-3977.
12. Davies, J., Yildiz, F. & Grossman, A. R. 1996. Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO Journal, 15:2150-2159.
13. Debuchy, R., Purton, S. & Rochaix, J.-D. 1989. The arginosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO Journal, 8:2803-2809.
14. Deiner, D. R., Curry, A. M., Johnson, K. A., Williams, B. D., Lefebvre, P. A., Kindle, K. L. & Rosenbaum, J. L. 1990. Rescue of a paralyzed flagella mutant of Chlamydomonas by transformation. Proc. Natl. Acad. Sci. USA, 87:5739-5743.
15. Dunahay, T. G. 1993. Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers. BioTechniques, 15:452-460
16. Dutcher, S. K. 1986. Genetic properties of linkage group XIX in Chlamydomonas reinhardtii. In: Wickner, R. B., Hinnebusch, A., Lambowitz, A. M., Gunsalus, I. C. & Hollaender, A. (ed.), Extrachromosomal Elements in Lower Eukaryotes. Plenum Press, New York.
17. Dutcher, S. K. 1995. Flagellar assembly in 250 easy-to-follow steps. Trends Genet., 11:398-404.
18. Dutcher, S. K. & Trabuco, E. C. 1998. The UNI3 gene is required for the assembly of basal bodies in Chlamydomonas and encodes delta tubulin, a new member of the tubulin superfamily. Mol. Biol. Cell, 9:1293-1308.
19. Dutcher, S. K., Gibbons, W. & Inwood, W. B. 1988. A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetic, 120:965-976.
20. Dutcher, S. K., Power, J., Galloway, R. E. & Porter, M. E. 1991. Reappraisal of the genetic map of Chlamydomonas reinhardtii. J. Hered., 82:295-301.
21. Ehler, L. E. & Dutcher, S. K. 1998. Pharmacological and genetic evidence for a role of rootlet and phycoplast microtubules in the positioning anc assembly of cleavage furrows in Chlamydomonas reinhardtii. Cell Motil. Cytoskeleton., 40:193-207.
22. Ehler, L. E., Holmes, J. A. & Dutcher, S. K. 1995. Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies. Genetics, 141:945-960.
23. Fernandez, E., Schnell, R. A., Ranum, L. P., W., Hussey, S. C. Silflow, C. D. & Lefebvre, P. A. 1989. Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA, 86:6449-6453.
24. Ferris, P. J. 1989. Characterization of a Chlamydomonas transposon, Gulliver, resembling those in higher plants. Genetics, 122:363-377.
25. Finst, R. J., Kim, P. J. & Quarmby, L. M. 1998. Genetics of the deflagellation pathway in Chlamydomonas. Genetics, 149:927-936.
26. Forest, C. L. & Togasaki, R. K. 1977. A selection procedure for obtaining conditional gametogenic mutants using a photosyntheically incompentent strain of Chlamydomonas reinhardtii. Mol. Gen. Genet., 153:227-230.
27. Gaffel, G. P. & el-Gammel, S. 1990. Elucidation of the enigma of the "metaphase band" of Chlamydomonas reinhardtii. Protoplasma, 156:139-148.
28. Gardner, L. C., O'Toole, E., Perrone, C. A., Giddings, T. & Porter, M. E. 1994. Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella. J. Cell Biol., 127:1311-1325.
29. Goodenough, U. W. & St. Clair, H. S. 1975. BALD2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii. J. Cell Biol., 66:480-491.
30. Gumpel, N. J., Rochaix, J. D. & Purton, S. 1994. Studies on homologous recombination in the green alga Chlamydomonas reinhardtii. Curr. Genet. 26:438-442.
31. Habermacher, G. & Sale, W. S. 1997. Regulation of a flagellar dynein by phosphorylation of a 138-kD inner dynein arm intermediate chain. J. Cell Biol., 136:167-176.
32. Hails, T., Huttner, O. & Day, A. 1993. Isolation of a Chlamydomonas reinhardtii telomere by functional complementation in yeast. Curr. Genet., 28:437-440.
33. Harris, E. H. 1989. The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use. Academic Press, San Diego.
34. Holmes, J. A., Johnson, D. E. & Dutcher, S. K. 1993. Linkage group XIX of Chlamydomonas reinhardtii has a linear map. Genetics. 133: 865-874.
35. Horst, C. J. & Witman, G. B. 1993. ptx1, a nonphototactic mutant of Chlamydomonas lacks control of flagellar dominance. J. Cell Biol., 120:733-741.
36. Huang, B., Piperno, G. & Luck, D. J. L. 1979. Paralyzed flagella mutants of Chlamydomonas reinhardtii defective for axonemal doublet microtubule arms. J. Biol. Chem., 254:3091-3099.
37. Huang, B., Piperno, G. & Luck, D. J. L. 1981. Radial spokes of Chlamydomonas flagella. Genetic analysis of assembly and function. J. Cell Biol., 88:80-88.
38. Huang, B., Ramanis, Z. & Luck, D. J. L. 1982a. Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for flagellar function. Cell, 28:115-124.
39. Huang, B., Rifkin, M. R. & Luck, D. J. L. 1977. Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J. Cell Biol., 72:67-85.
40. Huang, B., Ramanis, Z., Dutcher, S. K. & Luck, D. J. L. 1982b. Uniflagellar mutants of Chlamydomonas. Evidence for the role of basal bodies in transmission of positional information. Cell, 29:745-753.
41. Hummel, K. P. & Chapman, D. B. 1956. Visceral inversion and associated anomalies in the mouse. J. Heret., 50:9-13.
42. James, S. W. & Lefebvre, P. A. 1992. Genetic interactions among Chlamydomonas reinhardtii mutations that confer resistance to anti-microtubule herbicides. Genetics, 130:305-314.
43. James, S. W., Silflow, C. D., Stroom, P. & Lefebvre, P. A. 1993. A mutation in the alpha1-tubulin gene of Chlamydomonas reinhardtii confers resistance to anti-microtubule herbicides. J. Cell Sci., 106: 209-218.
44. James, S. W., Silflow, C. D., Thompson, M. D., Ranum, L. P. W. & Lefebvre, P. A. 1989. Extragenic suppression and synthetic lethality among Chlamydomonas reinhardtii mutants resistant to anti-microtubule drugs. Genetics, 122:567-577.
45. Kamiya, R. 1988. Mutations at twelve independent loci result in the absence of the outer dyenin arms in Chlamydomonas reinhardtii. J. Cell Biol., 107:2253-2258.
46. Kamiya, R., Kurimoto, E. & Muto, E. 1991. Two types of Chlamydomonas flagellar mutants missing different components of the inner-arm dynein. J. Cell Biol., 112:441-447.
47. Kato, T., Kagami, O., Yagi, T. & Kamiya, R. 1993. Isolation of two species of Chlamydomonas reinhardtii flagellar mutants, ida5 and ida6, that lack a newly identified heavy chain of the inner dynein arm. Cell Struct. Funct., 18:371-377.
48. Kindle, K. L. 1990. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA, 87:1228-1232.
49. Kindle, K. L., Schnell, R. A., Fernandez, E. & Lefebvre, P. A. 1989. Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J. Cell Biol., 109:2589-2601.
50. King, S. J. & Dutcher, S. K. 1997. Phosphoregulation of an inner dynein arm complex in Chlamydomonas reinhardtii is altered in phototactic mutant strains. J. Cell Biol., 136:177-191.
51. King, S. J., Inwood, W. B., O'Toole, E., Power, J. & Dutcher, S. K. 1994. The bop2-1 mutation reveals radial asymmetry in the inner dynein arm region of Chlamydomonas reinhardtii. J. Cell Biol., 126: 1255-1266.
52. King, S. M., Barbarese, E., Dillman, J. F. III, Benashski, S. E., Do, K. T., Patel-King, R. S. & Pfister, K. K. 1998. Cytoplasmic dynein contains a family of differentially expressed light chains. Biochemistry, 37:15033-15041.
53. Koutoulis, A., Pazour, G. J., Wilkerson, C. G., Inaba, K., Sheng, H., Takada, S. & Witman, G. B. 1997. The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex. J. Cell Biol., 137:1069-1080.
54. Kozminski, K. G., Johnson, K. A., Forscher, P. & Rosenbaum, J. L. 1993. A motility in the eukaryotic flagellum unrelated to flagellar beating. Proc. Natl. Acad. Sci. USA, 90:5519-5523.
55. Kozminski, K. G., Beech, P. L. & Rosenbaum, J. L. 1995. The Chlamydomonas kinesin-like protein Fla10 is involved in motility associated with the flagellar membrane. J. Cell Biol., 131:1517-1527.
56. Kuchka, M. R. & Jarvik, J. W. 1982. Analysis of flagellar size control using a mutant of Chlamydomonas reinhardtii with a variable number of flagella. J. Cell Biol., 92:170-175.
57. Lechtreck, K. F. & Silflow, C. D. 1997. SF-assemblin in Chlamydomonas: sequence conservation and localization during the cell cycle. Cell Motil. Cytoskeleton, 36:190-201.
58. LeDizet, M. & Piperno, G. 1995. The light chain p28 associates with a subet of inner dynein arms heavy chains in Chlamydomonas axonemes. Mol. Biol. Cell, 6:697-711.
59. Lux, F. G. III & Dutcher, S. K. 1991. Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii. Genetics, 128: 549-561.
60. Marszalek, J. R., Ruiz-Lozano, P., Roberts, E., Chien, K. R. & Goldstein. L. S. B. 1999. Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc. Natl. Acad. Sci. USA, 96:5043-5048.
61. Mastronarde, D. N., O'Toole, E. T., McDonald, K. L., McIntosh, J. R. & Porter, M. E. 1992. Arrangement of inner dynein arms in wild-type and mutant flagella of Chlamydomonas. J. Cell Biol., 118:1145-1162.
62. McVittie, A. 1972. Flagellum mutants of Chlamydomonas reinhardtii. J. Gen. Microbiol, 71:525-540.
63. Mitchell, D. R. & Kang, Y. 1991. Identification of oda6 as a Chlamydomonas dynein mutant by rescue with the wild-type gene. J. Cell Biol., 113:835-842.
64. Mitchell, D. R. & Sale, W. S. 1999. Characterization of a Chlamydomonas insertional mutant that disrupts flagellar central pair microtubule-associated structures. J. Cell Biol., 144:293-304.
65. Myster, S. H., Knott, J. A., O'Toole, E. & Porter, M. E. 1997. The Chlamydomonas DHCI gene encodes a dynein heavy chain subunit required for the assembly of the II inner arm complex. Mol. Biol. Cell, 8:607-620.
66. Nonaka, S., Tanaka, Y., Okada, Y., Takeda, S., Harada, A., Kanai, Y., Kido, M. & Hirokawa, N. 1998. Randomization of left-right asymmetry due to due of nodal cilia generating leftward flow of extra-embryonic fluid in mice lacking Kif3B motor protein. Cell, 95:829-838.
67. Nelson, J. A. E. & Lefebvre, P. A. 1995. Targeted disruption of the NIT8 gene in Chlamydomonas reinhardtii. Mol. Cell. Biol., 15:5762-5769.
68. Nelson, J. A. E., Savereide, P. B. & Lefebvre, P. A. 1994. The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation. Mol. Cell. Biol., 14: 4011-4019.
69. Ohara, A., Kato-Minoura, T., Kamiya, R. & Hirono, M. 1998. Recovery of flagellar inner-arm dynein and the fertilization tubule in Chlamydomonas ida5 mutant by transformation with the actin gene. Cell. Struct. Funct., 23:272-281.
70. Palombella, A. L. & Dutcher, S. K. 1998. Identification of the gene encoding tryptophan synthetase β-subunit from Chlamydomonas reinhardtii. Plant Physiol., 117:455-464.
71. Pazour, G. J., Dickert, B. L. & Witman, G. B. 1999. The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly. J. Cell Biol., 144:473-481.
72. Pazour, G. J., Sineshchekov, O. A. & Witman, G. B. 1995. Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J. Cell Biol., 131:427-440.
73. Pazour, G. J., Wilkerson, C. G. & Witman, G. B. 1998. A dynein light chain is essential for retrograde particle movement of intraflagellar transport (IFT). J. Cell Biol., 141:979-992.
74. Perrrone, C. A., Yang, P., O'Toole, E., Sale, W. S. & Porter, M. E. 1998. The Chlamydomonas IDA7 locus encodes a 140-kD dynein intermediate chain required to assemble the I1 inner arm complex. Mol. Biol. Cell, 9:3351-3365.
75. Piperno, G. & Mead, K. 1997. Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella. Proc. Natl. Acad. Sci. USA, 94:4457-4462.
76. Piperno, G., Huang, B. & Luck, D. J. L. 1977. Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA, 74:1600-1604.
77. Piperno, G., Mead, K. & Henderson, S. 1996. Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1 (FLA10) to reach the distal part of flagella in Chlamydomonas. J. Cell Biol., 133:371-379.
78. Piperno, G., Mead, K., LeDizet, M. & Moscatelli, A. 1994. Mutations in the "dynein regulatory complex" alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes. J. Cell Biol., 125:1109-1117.
79. Piperno, G., Ramanis, Z., Smith, E. F. & Sale, W. S. 1990. Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme. J. Cell Biol., 110:379-389.
80. Piperno, G., Siuda, E., Henderson, S., Sefil, M., Vaanane, H. & Sassaroli, M. 1998. Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects. J. Cell Biol., 143:1591-1601.
81. Porter, M. E., Bower, R., Knott, J. A., Byrd, P. & Dentler, W. 1999. Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas. Mol Biol. Cell, 10:693-712.
82. Porter, M. E., Knott, J. A., Gardner, L. C., Mitchell, D. R. & Dutcher, S. K. 1994. Mutations in the SUP-PF-1 locus of Chlamydomonas reinhardtii identify a regulatory domain in the β-dynein heavy chain. J. Cell Biol., 126:1495-1507.
83. Purton, S. & Rochaix, J.-D. 1994. Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library. Plant Mol. Biol., 24:533-537.
84. Ramanis, Z. & Luck, D. J. L. 1986. Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomanas reinhardtii. Proc. Natl. Acad. Sci. USA, 83:423-426.
85. Ringo, D. L. 1967. Flagellar motion and fine structure of the flagellar apparatus of Chlamydomonas. J. Cell Biol., 33:543-571.
86. Rupp, G., O'Toole, E., Gardner, L. C., Mitchell, B. F. & Porter, M. E. 1996. The sup-pf-2 mutations of Chlamydomonas alter the activity of the outer dynein arms by modification of the gamma-dynein heavy chain. J. Cell Biol., 135:1853-1865.
87. Sakakibara, H., Mitchell, D. & Kamiya, R. 1991. A Chlamydomonas outer arm dynein mutant missing the α heavy chain. J. Cell Biol., 113:615-622.
88. Segal, R. A., Huang, B., Ramanis, Z. & Luck, D. J. L. 1984. Mutant strains of Chlamydomonas reinhardtii that move backwards only. J. Cell Biol., 98:2026-2034.
89. Shimogawara, K., Fujiwara, S., Grossman, A. R. & Usuda, H. 1998. High efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics, 148:1821-1828
90. Silflow, C. D., LaVoie, M., Wu, W. & Tam, L.-W. 1998. The product of the VFL1gene in Chlamydomonas localizes to the basal bodies. Mol. Biol., Cell 9:398a.
91. Small, G. D., Min, B. & Lefebvre, P. A. 1995. Characterization of a Chlamydomonas reinhardtii gene encoding a protein of the DNA photolyase/blue light photoreceptor family. Plant Mol. Biol., 28:443-454.
92. Smith, E. F. & Lefebvre, P. A. 1996. PF16 encodes a protein with armadillo repeats and localizes to a single microtubule of the central apparatus in Chlamydomonas flagella. J. Cell Biol., 132:359-370.
93. Smith, E. F. & Lefebvre, P. A. 1997. PF20 gene product contains WD repeats and localizes to the intermicrotubule bridges in Chlamydomonas flagella. Mol. Biol. Cell, 8:455-467.
94. Stevens, D. R., Rochaix, J.-D. & Purton, S. 1996. The bacterial phleornycin resistance gene ble as a dominant selectable marker in Chlamydomonas. Mol. Gen. Genet., 251:23-30.
95. Supp, D. M., Eitte, D. P., Potter, S. S. & Brueckner, M. 1997. Mutations of an axonemal dynein affects left-right asymmetry in inversus viscerum mice. Nature, 389:963-966.
96. Taillon, B. E., Adler, S. A., Suhan, J. P. & Jarvik, J. W. 1992. Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas. J. Cell Biol., 119:1613-1624.
97. Tam, L.-W. & Lefebvre, P. A. 1993. Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics, 135:375-384.
98. Walther, Z., Vashishtha, M. & Hall, J. L. 1994. The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein. J. Cell Biol., 126:175-188.
99. Wang, S. C., Schnell, R. A. & Lefebvre, P. A. 1998. Isolation and characterization of a new transposable element in Chlamydomonas reinhardtii. Plant Mol. Biol., 38:681-687.
100. r intermediate chain of Chlamydomonas outer arm dynein is a WD-repeat protein required for arm assembly. J. Cell Biol., 129:169-178.
101. Wright, R. L., Salisbury, J. L. & Jarvik, J. W. 1985. A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation. J. Cell Biol., 101:1903-1912.
102. Yang, P. & Sale, W. S. 1998. The Mr 140,000 intermediate chain of Chlamydomonas flagella inner arm dynein is a WD-repeat protein implicated in dynein arm anchoring. Mol. Biol. Cell, 9:3335-3349.
103. Zhang, H., Herman, P. L. & Weeks, D. P. 1994. Gene isolation through genomic complementation using an indexed library of Chlamydomonas reinhardtii DNA. Plant Mol. Biol. 24:663-672.