The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas

Molecular and General Genetics

Volumn 251, Issue 1, 1996, Pages 23-30

  Stevens, David R ^a   Rochaix, Jean David ^b   Purton, Saul ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

ble; Chlamydomonas; Dominant marker; Transformation

Indexed keywords

ANTIBODY; PHLEOMYCIN;

ARTICLE; BACTERIAL GENE; CELL GROWTH; CELL TRANSFORMATION; CHIMERA; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; DRUG RESISTANCE; GENE EXPRESSION; GENE FUSION; GENETIC ANALYSIS; IMMUNOBLOTTING; MARKER GENE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL;

ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; BIOLOGICAL MARKERS; BLOTTING, SOUTHERN; CHLAMYDOMONAS REINHARDTII; DNA PRIMERS; DRUG RESISTANCE, MICROBIAL; GENES, BACTERIAL; GENES, PLANT; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES, ANTISENSE; PHENOTYPE; PHLEOMYCINS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RIBULOSE-BISPHOSPHATE CARBOXYLASE; TRANSFECTION; TRANSFORMATION, GENETIC;

EID: 0029883758     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050135     Document Type: Article

References (45)

1. Adam M, Lentz KE, Loppes R (1993) Insertional mutagenesis to isolate acetate-requiring mutants in Chlamydomonas reinhardtii. FEMS Microbiol Lett 110 : 265-268
2. - strain. Mol Cell Biol 12 : 5268-5279
3. Bingham SE, Cox JC, Strem MD (1989) Expression of foreign DNA in Chlamydomonas reinhardtii. FEMS Microbiol Lett 65 : 77-82
4. 2-tubulin promoter in Chlamydomonas reinhardtii. Nucleic Acids Res 20 : 2959-2965
5. Davies JP, Yildiz F, Grossman AR (1994) Mutants of Chlamydomonas with aberrant responses to sulfur deprivation. Plant Cell 6 : 53-63
6. Day A, Debuchy R, van Dillewjin J, Purton S, Rochaix J-D (1990) Studies on the maintenance and expression of cloned DNA fragments in the nuclear genome of the green alga Chlamydomonas reinhardtii. Physiol Plant 78 : 254-260
7. Debuchy R, Purton S, Rochaix J-D (1989) The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J 8 : 2803-2809
8. Diener DR, Curry AM, Johnson KA, Williams BD, Lefebvre PA, Kindle KL, Rosenbaum JL (1990) Rescue of a paralyzed-flagella mutant of Chlamvdomonas by transformation. Proc Natl Acad Sci USA 87 : 5739-5743
9. Drocourt D, Calmels T, Reynes JP, Baron M, Tiraby G (1990) Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes. Nucleic Acids Res 18 : 4009
10. Dumas P, Bergdoll M, Cagnon C, Masson J-M (1994) Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering. EMBO J 13 : 2483-2492
11. Feinberg AP, Vogelstein B (1984) A technique for radiolabeling DNA restriction nuclease fragments to high specific activity. (Addendum). Anal Biochem 137 : 266-267
12. Gatignol A, Durand H, Tiraby G (1988) Bleomycin resistance conferred by a drug-binding protein. FEBS Lett 230 : 171-175
13. Goldschmidt-Clermont M, Rahire (1986) Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamvdomonas reinhardtii. J Mol Biol 191 : 421-432
14. Gorman DS, Levine RP (1965) Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamvdomonas reinhardtii. Proc Natl Acad Sci USA 54: 1665-1669
15. Gumpel NJ, Purton S (1994) Playing tag with Chlamydomonas. Trends Cell Biol 4 : 299-301
16. Gumpel NJ, Rochaix J-D, Purton S (1994) Studies on homologous recombination in the nucleus of Chlamvdomonas reinhardtii. Curr Genet 26 : 438-442
17. b f complex. Plant Mol Biol 29 : 921-932
18. Hall LM, Taylor KB, Jones DD (1993) Expression of a foreign gene in Chlamydomonas reinhardtii. Gene 124 : 75-81
19. Harris EH (1989) The Chlamydomonas sourcebook: a comprehensive guide to biology and laboratory use. Academic Press, San Diego
20. Hasnain SE, Manavathu EK, Leung W-C (1985) DNA-mediated transformation of Chlamydomonas reinhardtii cells: use of amino-glycoside 3′-phosphotransferase as a selectable marker. Mol Cell Biol 5 : 3647-3650
21. Jain S, Durand H, Tiraby G (1992) Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker. Mol Gen Genet 234 : 489-493
22. Jefferies D, Tebabi P, Le Ray D, Pays E (1993) The Me resistance gene as a new selectable marker for Trypanosoma brucei: fly transmission of stable procyclic transformants to produce antibiotic-resistant bloodstream forms. Nucleic Acids Res 21 : 191-195
23. Kindle KL (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 87 : 1228-1232
24. Kindle KL, Sodeinde OA (1994) Nuclear and chloroplast transformation in Chlamydomonas reinhardtii: strategies for genetic manipulation and gene expression. J Appl Phycol 6 : 231-238
25. Kindle KL, Schnell RA, Fernández E, Lefebvre PA (1989) Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J Cell Biol 109 : 2589-2601
26. Kozminski KG, Diener DR, Rosenbaum JL (1993) High level expression of nonacetylatable α-tubulin in Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 25 : 158-170
27. Mayfield SP, Kindle KL (1990) Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker. Proc Natl Acad Sci USA 87 : 2087-2091
28. McHugh JP, Spanier JG (1994) Isolation of cadmium-sensitive mutants in Chlamydomonas reinhardtii by transformation/insertional mutagenesis. FEMS Micro Lett 124 : 239-244
29. Mitchell DR, Kang Y (1991) Identification of oda6 as a Chlamydomonas dynein mutant by rescue with the wild-type gene. J Cell Biol 113 : 835-842
30. Nelson JAE, Savereide PB, Lefebvre PA (1994) The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation. Mol Cell Biol 14 : 4011-4019
31. Poljak L, Seum C, Mattioni T, Laemmli UK (1994) SARs stimulate but do not confer position-independent gene expression. Nucl Acids Res 22 : 4386-4394
32. Purton S, Rochaix J-D (1994) Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library. Plant Mol Biol 24 : 533-537
33. Purton S, Rochaix J-D (1995) Characterization of the ARG7 gene of Chlamydomonas reinhardtii and its application to nuclear transformation. Eur J Phycol 30 : 141-148
34. Quinn J, Li HH, Singer J, Morimoto B, Mets L, Kindle K, Merchant S (1993) The plastocyanin-deficient phenotype of Chlamydomonas reinhardtii Ac-208 results from a frameshift mutation in the nuclear gene encoding apopreplastocyanin. J Biol Chem 268 : 7832-7841
35. Rochaix J-D (1987) Molecular genetics of chloroplasts and mitochondria in the unicellular green alga Chlamydomonas. FEMS Microbiol Rev 46 : 13-34
36. Rochaix J-D, van Dillewijn J (1982) Transformation of the green alga Chlamydomonas reinhardtii with yeast DNA. Nature 296 : 70-72
37. Rochaix J-D, Mayfield S, Goldschmidt-Clermont M, Erickson J (1988) Molecular biology of Chlamydomonas In: Schaw C-H (ed) Plant Molecular Biology: A Practical Approach. IRL Press, Oxford
38. Sambrook J, Frisch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
39. Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulphate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1-100 kDa. Anal Biochem 166 : 368-379
40. Smart EJ, Selman BR (1993) Complementation of a Chlamydomonas reinhardtii mutant defective in the nuclear gene encoding the chloroplast coupling factor 1 (CF1) γ-subun t (atpC). J Bioenerg Biomemb 3 : 275-284
41. Sodeinde OA, Kindle KL (1993) Homologous recombination in the nuclear genome of Chlamydomonas reinharatii. Proc Natl Acad Sci USA 90 : 9199-9203
42. Stevens DR, Purton S (1994) Expression of foreign genes in the green alga Chlamydomonas reinhardtii. J Exp Bot 45 (Suppl) : 20
43. Tarn LW, Lefebvre PA (1993) Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics 135 : 375-384
44. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 : 4350-4354
45. Zhang H, Herman PL, Weeks DP (1994) Gene isolation through genomic complementation using an indexed library of Chlamydomonas reinhardtii DNA. Plant Mol Biol 24 : 663-672