Bacterial and nonbacterial expression of wild-type and mutant human phospholipid hydroperoxide glutathione peroxidase and purification of the mutant enzyme in the milligram scale

Protein Expression and Purification

Volumn 19, Issue 3, 2000, Pages 403-410

  Schnurr, Kerstin ^a   Borchert, Astrid ^a   Gerth, Christa ^a   Anton, Monika ^a   Kuhn, Hartmut ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CODON; ENZYME PURIFICATION; HYBRID PROTEIN; INSECT CELL CULTURE; LIPID METABOLISM; MUTANT; MUTATION; PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE; SELENOCYSTEINE; WILD RELATIVE;

ESCHERICHIA COLI;

EID: 0033843992     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1262     Document Type: Article

References (35)

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4. Regulation of selenoproteins
5. Dissipation of mitochondrial membrane potential by exogenous phospholipid monohydroperoxide and protection against this effect by transfection of cells with phospholipid hydroperoxide glutathione peroxidase gene
6. Overexpression of phospholipid hydroperoxide glutathione peroxidase suppressed cell death due to oxidative damage in rat basophile leukemia cells (RBL-2H3)
7. Distribution and possible novel role of phospholipid hydroperoxide glutathione peroxidase in rat epididymal spermatozoa
8. Comparison of age-related differences in expression of phospholipid hydroperoxide glutathione peroxidase mRNA and activity in various tissues of pigs
9. Testosterone mediates expression of the selenoprotein PHGPx by induction of spermatogenesis and not by direct transcriptional gene activation
10. Dual function of the selenoprotein PHGPx during sperm maturation
11. Expression pattern of phospholipid hydroperoxide glutathione peroxidase messenger ribonucleic acid in mouse testis
12. Puberty influences expression of phospholipid hydroperoxide glutathione peroxidase (GPX4) in rat testis: Probable hypophysis regulation of the enzyme in male reproductive tract
13. Selenium-dependent peroxidases suppress 5-lipoxygenase activity in B-lymphocytes and immature myeloid cells: The presence of peroxidase-insensitive 5-lipoxygenase activity in differentiated myeloid cells
14. Suppression of leukotriene formation in RBL-2H3 cells that overexpressed phospholipid hydroperoxide glutathione peroxidase
15. The selenoenzyme phospholipid hydroperoxide glutathione peroxidase controls the activity of the 15-lipoxygenase with complex substrates and preserves the specificity of the oxygenation products
16. Identification of a lipoxygenase inhibitor in A431 cells as a phospholipid hydroperoxide glutathione peroxidase
17. Phospholipid hydroperoxide glutathione peroxidase
18. Enzymatic and immunological measurements of soluble and membrane-bound phospholipid-hydroperoxide glutathione peroxidase
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20. Knowing when not to stop: Selenocysteine incorporation in eukaryotes
21. Substitution of cysteine for selenocysteine in type I iodothyronine deiodinase reduces the catalytic efficiency of the protein but enhances its translation
22. Diversity of glutathione peroxidases
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24. The deiodinase family of selenoproteins
25. Human mitochondrial thioredoxin reductase cDNA cloning, expression and genomic organization
26. Identification of a novel selD homolog from eukarycytes, bacteria, and archaea: Is there an autoregulatory mechanism in selenocysteine metabolism?
27. Orphan selenoproteins
28. Phospholipid hydroperoxide glutathione peroxidase (PHGPx): More than an antioxidant enzyme?
29. High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial type SECIS elements and co-expression with the selA, selB and selC genes
30. Probing the presumed catalytic triad of a selenium-containing peroxidase by mutational analysis
31. Interleukin-1 induced nuclear factor kappa B activation is inhibited by overexpression of phospholipid hydroperoxide glutathione peroxidase in a human endothelial cell line