Irreversible heat inactivation of DNase I without RNA degradation

BioTechniques

Volumn 29, Issue 2, 2000, Pages 252-256

  Wiame, I ^a   Remy, S ^a   Swennen, R ^a   Sagi, L ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE I; EDETIC ACID; EGTAZIC ACID; RESTRICTION ENDONUCLEASE; RNA;

ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; ENZYME INACTIVATION; GENE AMPLIFICATION; GENOME; HEATING; NONHUMAN; PLANT LEAF; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION;

EID: 0033840632     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00292bm11     Document Type: Article

References (10)

1. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development
2. Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion
3. Magnesium-dependent thermostability of DNase I
4. Reactions of polynucleotides and nucleic acids
5. Restriction endonuclease digestion eliminates product contamination in reverse transcribed polymerase chain reaction
6. Use of reverse transcriptase polymerase chain reaction to monitor expression of intronless genes
7. Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR
8. Distribution and cloning of eukaryotic mRNAs by means of differential display: Refinements and optimization
9. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase