Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes

BioTechniques

Volumn 29, Issue 2, 2000, Pages 310-313

  Zhu, F ^a   Yan, W ^a   Zhao, Z L ^a   Chai, Y B ^a   Lu, F ^a   Wang, Q ^a   Peng, W D ^a   Yang, A G ^a   Wang, C J ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA FRAGMENT; STREPTAVIDIN; TUMOR NECROSIS FACTOR;

APOPTOSIS; ARTICLE; CELL STRAIN HL 60; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DNA LIBRARY; DOT HYBRIDIZATION; GENE EXPRESSION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMYELOCYTIC LEUKEMIA;

ATRA;

EID: 0033836154     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00292st06     Document Type: Article

References (11)

1. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates
2. Isolation, analysis, and synthesis. A new method for full-length cDNA cloning by PCR
3. Protective effects of notch-1 on TCR-induced apoptosis
4. Positive selection of candidate tumor-suppressor genes by subtractive hybridization
5. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
6. Subtractive cloning: Past, present, and future
7. Synthesis of cDNA strain
8. Hybridization to nitrocellulose filters with isotopes labeled probes
9. Necdin, a postmitotic neuron-specific growth suppressor, interacts with viral transforming proteins and cellular transcription factor E2F1
10. Human immunodeficiency virus type 1 Nef protein sensitizes CD4(+) T lymphoid cells to apoptosis via functional upregulation of the CD95/CD95 ligand pathway
11. Defects of differential display technique and strategies to deal with problems