Characterization of the interaction of IpaB and IpaD, proteins required for entry of Shigella flexneri into epithelial cells, with a lipid membrane

European Journal of Biochemistry

Volumn 267, Issue 18, 2000, Pages 5769-5776

  De Geyter, Carine ^a   Wattiez, Ruddy ^b   Sansonetti, Philippe ^c   Falmagne, Paul ^b   Ruysschaert, Jean Marie ^a   Parsot, Claude ^c   Cabiaux, Véronique ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^b UNIVERSITY OF MONS   (Belgium)

^c INSTITUT PASTEUR   (France)

Author keywords

Membrane; Protein lipid interaction; Shigella flexneri; Type III secretion

Indexed keywords

BACTERIAL PROTEIN; LIPOSOME; MEMBRANE LIPID; PROTEIN IPAB; PROTEIN IPAC; PROTEIN IPAD; UNCLASSIFIED DRUG;

ARTICLE; EPITHELIUM CELL; LIPID MEMBRANE; NONHUMAN; PH; PHAGOSOME; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LIPID INTERACTION; PROTEIN PURIFICATION; PROTEIN SECRETION; SHIGELLA FLEXNERI;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; CELL MEMBRANE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOSOMES; EPITHELIAL CELLS; FLUORESCEINS; HYDROGEN-ION CONCENTRATION; KINETICS; LIPID METABOLISM; LIPOSOMES; PHOSPHATIDYLCHOLINES; PHOSPHOLIPIDS; PROTEIN BINDING; SHIGELLA FLEXNERI; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SHIGELLA FLEXNERI;

EID: 0033825366     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01649.x     Document Type: Article

References (34)

1. Invasion and the pathogenesis of Shigella infections
2. Cloning of plasmid DNA sequences involved in invasion of HeLa cells by Shigella flexneri
3. Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a
4. MxiG, a membrane protein required for secretion of Shigella spp. Ipa invasins: Involvement in entry into epithelial cells and in intercellular dissemination
5. Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus
6. MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri Ipa invasins
7. MxiJ, a lipoprotein involved in secretion of Shigella Ipa invasins, is homologous to YscJ, a secretion factor of the Yersinia Yop proteins
8. Two novel virulence loci, mxiA and mxiB, in Shigella flexneri 2a facilitate excretion of invasion plasmid antigens
9. The mxi-Spa type III secretory pathway of Shigella flexneri requires an outer membrane lipoprotein, MxiM, for invasin translocation
10. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells
11. Extracellular association and cytoplasmic partitioning of the IpaB and IpaC invasins of S. flexneri
12. The secreted Ipa complex of Shigella flexneri promotes entry into mammalian cells
13. Functional analysis of the Shigella flexneri IpaC invasin by insertional mutagenesis
14. IpaB of Shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole
15. Purification of IpaC, a protein involved in entry of Shigella flexneri into epithelial cells and characterization of its interaction with lipid membranes
16. Involvement of a plasmid in the invasive ability of Shigella flexneri
17. SepA, the major extracellular protein of Shigella flexneri: Autonomous secretion and involvement in tissue invasion
18. The secreted IpaB and IpaC invasins and their cytoplasmic chaperone IpgC are required for intercellular dissemination of Shigella flexneri
19. Partial resolution of the enzymes catalyzing oxidative phosphorylation
20. pH-Dependent bilayer destabilization and fusion of phospholipidic large unilamellar vesicles induced by diphtheria toxin and its fragments A and B
21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
22. Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped Volume and ability to maintain a membrane potential
23. A simplified procedure for lipid phosphorus analysis shows that digestion rates vary with phospholipid structure
24. Shigella flexneri: Isolation of noninvasive mutants of gram-negative pathogens
25. Enhanced secretion through the Shigella flexneri Mxi-Spa translocon leads to assembly of extracellular proteins into macromolecular structures
26. Induction of type III secretion in Shigella flexneri is associated with differential control of transcription of genes encoding secreted proteins
27. Physicochemical determinants for the interactions of magainins 1 and 2 with acidic lipid bilayers
28. Hypelcin A, an alpha-aminoisobutyric acid containing antibiotic peptide, induced permeability change of phosphatidylcholine bilayers
29. Secretion of Ipa proteins by Shigella flexneri: Inducer molecules and kinetics of activation
30. Entry of Shigella flexneri into HeLa cells: Evidence for directed phagocytosis involving actin polymerization and myosin accumulation
31. Interaction with a lipid membrane: A key step in bacterial toxins virulence
32. The tripartite type III secreton of Shigella flexneri inserts IpaB and IpaC into host membranes
33. Modulation of bacterial entry into epithelial cells by association between vinculin and the Shigella IpaA invasin
34. SopB, a protein required for virulence of Salmonella dublin, is an inositol phosphate phosphatase