The secreted IpaB and IpaC invasins and their cytoplasmic chaperone IpgC are required for intercellular dissemination of Shigella flexneri

Cellular Microbiology

Volumn 1, Issue 2, 1999, Pages 183-193

  Page, Anne Laure ^a   Ohayon, Hélène ^b   Sansonetti, Philippe J ^a   Parsot, Claude ^a  

^a INSERM   (France)

^b INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL PROTEIN; CHAPERONE; HYBRID PROTEIN; IPAB PROTEIN, SHIGELLA; IPAC PROTEIN, SHIGELLA; IPGC PROTEIN, SHIGELLA; ISOPROPYL THIOGALACTOSIDE;

ARTICLE; CELL STRAIN CACO 2; ESCHERICHIA COLI; GENETICS; HELA CELL; HUMAN; IMMUNOBLOTTING; METABOLISM; PATHOGENICITY; PLASMID; SHIGELLA FLEXNERI;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; CACO-2 CELLS; ESCHERICHIA COLI; HELA CELLS; HUMANS; IMMUNOBLOTTING; ISOPROPYL THIOGALACTOSIDE; MOLECULAR CHAPERONES; PLASMIDS; RECOMBINANT FUSION PROTEINS; SHIGELLA FLEXNERI;

EID: 0033188152     PISSN: 14625814     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-5822.1999.00019.x     Document Type: Article

References (49)

1. Allaoui, A., Mounier, J., Prévost, M.C., Sansonetti, P.J., and Parsot, C. (1992) icsB: a S. flexneri virulence gene necessary for the lysis of protrusions during intercellular spread. Mol Microbiol 6: 1605-1616.
2. Allaoui, A., Sansonetti, P.J., and Parsot, C. (1993) MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri Ipa invasins. Mol Microbiol 7: 59-68.
3. Anderson, D.M., and Schneewind, O. (1999) Yersinia enterocolitica type III secretion: an mRNA signal that couples translation and secretion of YopQ. Mol Microbiol 31: 1139-1148.
4. Andrews, G.P., and Maurelli, AT. (1992) mxiA of S. flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium response protein, LcrD, of Yersinia pestis. Infect Immun 60: 3287-3295.
5. Bahrani, F.K., Sansonetti, P.J., and Parsot, C. (1997) Secretion of Ipa proteins by Shigella flexneri: inducer molecules and kinetics of activation. Infect Immun 65: 4005-4010.
6. Barzu, S., Nato, F., Rouyre, S., Mazié, J.C., Sansonetti, P.J., and Phalipon, A. (1993) Characterization of B-cell epitopes on IpaB, an invasion-associated antigen of Shigella flexneri: identification of an immunodominant domain recognized during natural infection. Infect Immun 61: 3825-3831.
7. Barzu, S., Benjelloun-Touimi, Z., Phalipon, A., Sansonetti, P., and Parsot, C. (1997) Functional analysis of the Shigella flexneri IpaC invasin by insertional mutagenesis. Infect Immun 65: 1599-1605.
8. Bernardini, M.L., Mounier, J., d'Hauteville, H., Coquis-Rondon, M., and Sansonetti, P.J. (1989) Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra and intercellular spread through interaction with F-actin. Proc Natl Acad Sci USA 86: 3867-3871.
9. Chen, Y., Smith, M.R., Thirumalai, K., and Zychlinsky, A. (1996) A bacterial invasin induces macrophage apoptosis by binding directly to ICE. EMBO J 15: 3853-3860.
10. Cossart, P., and Lecuit, M. (1998) Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: bacterial factors, cellular ligands and signaling. EMBO J17: 3797-3806.
11. De Geyter, C., Vogt, B., Benjelloun-Touimi, Z., Sansonetti, P.J., Ruysschaert, J.M., Parsot, C., et al. (1997) Purification of IpaC, a protein involved in entry of Shigella flexneri into epithelial cells and characterization of its interaction with lipid membranes. FEBS Lett 400: 149-154.
12. Demers, B., Sansonetti, P.J., and Parsot, C. (1998) Induction of type III secretion in Shigella flexneri is associated with differential control of transcription of genes encoding secreted proteins. EMBO J 17: 2894-2903.
13. Gottesman, S., Clark, W.P., and Maurizi, M.R. (1990) The ATP-dependent Clp protease of Escherichia coll. J Biol Chem 265: 7886-7893.
14. Hakansson, S., Schesser, K., Persson, C., Galyov, E.E., Rosqvist, R., Homble, F., et al. (1996) The YopB protein of Yersinia pseudotuberculosis is essential for the translocation of Yop effector proteins across the target cell plasma membrane and displays a contact-dependent membrane disrupting activity. EMBO J 15: 5812-5823.
15. High, N., Mounier, J., Prévost, M.C., and Sansonetti, P.J. (1992) IpaB of Shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole. EMBO J 11: 1991-1999.
16. Hueck, C.J. (1998) Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62: 379-433.
17. LaBrec, E.H., Schneider, H., Magnani, T.J., and Formal, S.B. (1964) Epithelial cell penetration as an essential step in pathogenesis of bacillary dysentery. J Bacteriol 88: 1503-1518.
18. Makino, S., Sasakawa, C., Kamata, K., Kurata, T., and Yoshikawa, M. (1986) A virulence determinant required for continuous reinfection of adjacent cells on large plasmid in Shigella flexneri 2a. Cell 46: 551-555.
19. Maurelli, A.T., Baudry, B., d'Hauteville, H., Hale, T.L., and Sansonetti, P.J. (1985) Cloning of plasmid DNA sequences involved in invasion of HeLa cells by Shigella flexneri. Infect Immun 49: 164-171.
20. Ménard, R., Sansonetti, P.J., and Parsot, C. (1993) Non polar mutagenesis of the ipa gene defines IpaB, IpaC and IpaD as effectors of Shigella flexneri entry into epithelial cells. J Bacteriol 175: 5899-5906.
21. Ménard, R., Sansonetti, P.J., and Parsot, C. (1994a) The secretion of the Shigella flexneri Ipa invasins is induced by the epithelial cell and controlled by IpaB and IpaD. EMBO J 13: 5293-5302.
22. Ménard, R., Sansonetti, P.J., Parsot, C., and Vasselon, T. (1994b) Extracellular association and cytoplasmic partitioning of the IpaB and IpaC invasins of Shigella flexneri. Cell 79: 515-525.
23. Ménard, R., Prévost, M.C., Gounon, P., Sansonetti, P.J., and Dehio, C. (1996) The secreted Ipa complex of Shigella flexneri promotes entry into mammalian cells. Proc Natl Acad Sci USA 93: 1254-1258.
24. Mounier, J., Vasselon, T., Hellio, R., Lesourd, M., and Sansonetti, P.J. (1992) Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole. Infect Immun 60: 237-248.
25. Neyt, C., and Cornelis, G.R. (1999) Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD. Mol Microbiol 31: 143-156.
26. Oaks, E.V., Wingfield, M.E., and Formal, S.B. (1985) Plaque formation by virulent Shigella flexneri. Infect Immun 48: 124-129.
27. Parsot, C., Ménard, R., Gounon, P., and Sansonetti, P.J. (1995) Enhanced secretion through the Shigella flexneri Mxi-Spa translocon leads to assembly of extracellular proteins into macromolecular structures. Mol Microbiol 16: 291-300.
28. Phalipon, A., Arondel, J., Nato, F., Rouyre, S., Mazie, J.C., and Sansonetti, P.J. (1992) Identification and characterization of B-cell epitopes of IpaC, an invasion-associated protein of Shigella flexneri. Infect Immun 60: 1919-1926.
29. Rosqvist, R., Magnusson, K.E., and Wolf-Watz, H. (1994) Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J 13: 964-972.
30. Sansonetti, P.J., and Egile, C. (1998) Molecular bases of epithelial cell invasion by Shigella flexneri. Antonie Van Leeuwenhoek 74: 191-197.
31. Sansonetti, P.J., Kopecko, D.J., and Formal, S.B. (1982) Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun 35: 852-860.
32. Sansonetti, P.J., Ryter, A., Clerc, P., Maurelli, AT., and Mounier, J. (1986) Multiplication of Shigella flexneri within HeLa cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. Infect Immun 51: 461-469.
33. Sasakawa, C., Kamata, K., Sakai, T., Makino, S., Yamada, M., Okada, N., et al. (1988) Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella flexneri 2a. J Bacteriol 170: 2480-2484.
34. Sasakawa, C., Adler, B., Tobe, T., Okada, N., Nagai, S., Komatsu, K., et al. (1989) Functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid of Shigella flexneri 2a. Mol Microbiol 3: 1191-1201.
35. Sasakawa, C., Komatsu, K., Tobe, T., Suzuki, T., and Yoshikawa, M. (1993) Eight genes in region-5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a. Infect Immun 175: 2334-2346.
36. Skarstad, K., Thöny, B., Hwangt, D.S., and Kornbergt, A. (1993) A novel binding protein of the origin of the Escherichia coli chromosome. J Biol Chem 268: 5365-5370.
37. Sory, M.P., and Cornelis, G.R. (1994) Translocation of a hybrid YopE-adenylate cyclase from Yersinia enterocolitica into HeLa cells. Mol Microbiol 14: 583-594.
38. Suzuki, T., Murai, T., Fukuda, I., Tobe, T., Yoshikawa, M., and Sasakawa, C. (1994) Identification and characterization of a chromosomal virulence gene, vacJ, required for intercellular spreading of Shigella flexneri. Mol Microbiol 11: 31-41.
39. Tilney, L.G., and Portnoy, D.A. (1989) Actin filaments and the growth, movement and spread of the intracellular bacterial parasite, Listeria monocytogenes. J Cell Biol 109: 1597-1608.
40. Tran Van Nhieu, G., and Sansonetti, P.J. (1999) Mechanism of Shigella entry into epithelial cells. Curr Opin Microbiol 2: 51-55.
41. Tran Van Nhieu, G., Ben-Ze'ev, A., and Sansonetti, P.J. (1997) Modulation of bacterial entry into epithelial cells by association between vinculin and the Shigella IpaA invasin. EMBO J 16: 2717-2729.
42. Tran Van Nhieu, G., Caron, E., Hall, A., and Sansonetti, P.J. (1999) IpaC induces actin polymerization and filopodia formation during Shigella entry into epithelial cells. EMBO J18: 3249-3262.
43. Vazquez-Boland, J.A., Kocks, C., Dramsi, S., Ohayon, H., Geoffroy, C., Mengaud, J., et al. (1992) Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. Infect Immun 60: 219-230.
44. Venkatesan, M.M., Buysse, J.M., and Oaks, E.V. (1992) Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus. J Bacteriol 174: 1990-2001.
45. Watarai, M., Tobe, T., Yoshikawa, M., and Sasakawa, C. (1995) Contact of Shigella with host cells triggers release of Ipa invasins and is an essential function of invasiveness. EMBO J14: 2461-2470.
46. Wattiau, P., Bernier, B., Deslee, P., Michiels, T., and Cornelis, G.R. (1994) Individual chaperones required for Yop secretion by Yersinia. Proc Natl Acad Sci USA 91: 10493-10497.
47. Wattiau, P., Woestyn, S., and Cornelis, G.R. (1996) Customized secretion chaperones in pathogenic bacteria. Mol Microbiol 20: 255-262.
48. Zierler, M.K., and Galan, J.E. (1995) Contact with cultured epithelial cells stimulates secretion of Salmonella typhimurium invasion protein InvJ. Infect Immun 63: 4024-4028.
49. Zychlinsky, A., Kenny, B., Ménard, R., Prévost, M.C., Holland, I.B., and Sansonetti, P.J. (1994) IpaB mediates macrophage apoptosis induced by Shigella flexneri. Mol Microbiol 11: 619-627.