Preparing poly A-containing RNA internal standards for multiplex competitive RT-PCR

BioTechniques

Volumn 29, Issue 3, 2000, Pages 454-458

  Celi, F S ^a   Mentuccia, D ^a   Proietti Pannunzi, L ^a   Di Gioia, C R T ^a   Andreoli, M ^a  

^a Dipto di Medicina Sperimentale   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

POLYADENYLATED RNA; RECEPTOR SUBTYPE; THYROID HORMONE RECEPTOR; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; MESSENGER RNA; POLYADENYLIC ACID; RNA; VIRUS PROTEIN;

ARTICLE; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; HUMAN CELL; METHODOLOGY; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA PURIFICATION; RNA SYNTHESIS; STANDARD; VALIDATION PROCESS; BINDING COMPETITION; BIOSYNTHESIS; CHEMISTRY; GENETICS; HEART MUSCLE; METABOLISM; NEEDLE BIOPSY; PLASMID; SENSITIVITY AND SPECIFICITY;

BINDING, COMPETITIVE; BIOPSY, NEEDLE; DNA-DIRECTED RNA POLYMERASES; HUMANS; MYOCARDIUM; PLASMIDS; POLY A; RECEPTORS, THYROID HORMONE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; VIRAL PROTEINS;

EID: 0033818220     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00293bm09     Document Type: Article

References (11)

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7. Quantitative analysis of human multidrug resistance 1 (MDR1) gene expression by non-isotopic competitive reverse transcriptase polymerase chain reaction assay
8. A synthetic standard for competitive RT-PCR quantitation of 13 GABA receptor type A subunit mRNAs in rats and mice
9. Highly specific and sensitive detection of malignancy in urine samples from patients with urothelial cancer by CD44v8-10/CD44v10 competitive RT-PCR
10. Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR
11. Technical aspects of quantitative competitive PCR