A synthetic standard for competitive RT-PCR quantitation of 13 GABA receptor type a subunit mRNAs in rats and mice

Journal of Neuroscience Methods

Volumn 85, Issue 1, 1998, Pages 89-98

  Liu, Zhi Fang ^a   Burt, David R ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Aminobutyric acid; GABA benzodiazepine receptors; Internal standard; mRNA quantitation; Polymerase chain reaction

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; RAT; RECEPTOR DENSITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION;

ANIMALS; MICE; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RATS; RECEPTORS, GABA-A; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0345345498     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(98)00125-3     Document Type: Article

References (34)

1. Apostolakos M.J., Schuermann W.H., Frampton M.W., Utell M.J., Willey J.C. Measurement of gene expression by multiplex competitive polymerase chain reaction. Anal Biochem. 213:1993;277-284
2. A receptor subunits. BioTechniques. 11:1991;636-641
3. A receptor-activated chloride channels. In: Guggino WG, editor. Chloride Channels. Current Topics in Membranes, vol. 42. New York: Academic Press, 1994:215-263.
4. A receptor subtypes: from pharmacology to molecular biology. FASEB J. 5:1991;2916-2923
5. Chelly J., Montarras D., Pinset C., Berwald-Netter Y., Kaplan J.C., Kahn A. Quantitative estimation of minor mRNAs by cDNA-polymerase chain reaction. Application to dystrophin mRNA in cultured myogenic and brain cells. Eur J Biochem. 187:1990;691-698
6. Don R.H., Cox P.T., Wainwright B.J., Baker K., Mattick J.S. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:1991;4008
7. Fasco M.J., Treanor C.P., Spivack S., Figge H.L., Kaminsky L.S. Quantitative RNA-polymerase chain reaction-DNA analysis by capilIary electrophoresis and laser-induced fluorescence. Anal Biochem. 224:1995;140-147
8. Ferre F. Quantitative or semi-quantitative PCR: reality versus myth. PCR Methods Appl. 2:1992;1-9
9. Grayson D.R., Bovolin P., Santi M.R. Absolute quantitation of γ-aminobutyric acid A receptor subunit messenger RNAs by competitive polymerase chain reaction. Methods Neurosci. 12:1993;191-208
10. Julien J.P., Meyer D., Flavell D., Hurst J., Grosveld F. Cloning and developmental expression of the murine neurofilament gene family. Brain Res. 387:1986;243-250
11. A receptors. Neuron. 3:1989;745-753
12. A receptors. J Neurochem. 56:1991;1717-1722
13. A-receptor confers sensitivity to benzodiazepine receptor ligands. FEBS Lett. 293:1991;191-194
14. A receptor γ2-subunit in mice by alternative splicing. J Neurochem. 56:1991;713-715
15. A receptor function. J Neurochem. 63:1994;1167-1170
16. Kuo C.F., Darnell J.E. Jr. Mouse glutamine synthetase is encoded by a single gene that can be expressed in a localized fashion. J Mol Biol. 208:1989;45-56
17. A receptor delta subunit mRNA level by tolerance to and withdrawal from pentobarbital. Neurosci Lett. 202:1996;149-152
18. A receptor selective for a behavioural alcohol antagonist. Nature. 346:1990;648-651
19. A receptor in rat brain. FEBS Lett. 260:1990;261-265
20. Martino T.A., Sole M.J., Penn L.Z., Liew C.C., Liu P. Quantitation of enteroviral RNA by competitive polymerase chain reaction. J Clin Microbiol. 31:1993;2634-2640
21. Reddy N.R., Wilkie B.N., Mallard B.A. Construction of an internal control to quantitate multiple porcine cytokine mRNAs by RT-PCR. BioTechniques. 21:1996;868-870
22. Scheuermann R.H., Bauer S.R. Polymerase chain reaction-based mRNA quantification using an internal standard: analysis of oncogene expression. Methods Enzymol. 218:1993;446-473
23. A receptor subunits exist in distinct neuronal subpopulations. Neuron. 3:1989;327-337
24. A receptor. Neurochem Int. 28:1996;137-139
25. Tokunaga K., Tanigachi H., Yoda K., Shimizu M., Sakiyama S. Nucleotide sequence of a full-length cDNA for mouse cytoskeletal β-actin mRNA. Nucleic Acids Res. 14:1986;2829
26. A receptor subunit mRNAs in 13 cell lines. J Neurosci. 14:1994;5417-5428
27. Wang A.M., Doyle M.V., Mark D.F. Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci USA. 86:1989;9717-9721
28. A receptor subtypes. Int Rev Neurobiol. 38:1995;95-138
29. A receptor subunit conferring atypical functional properties. J Neurosci. 17:1997;5027-5037
30. A receptor α4 subunit. FEBS Lett. 289:1991;227-230
31. A receptor β subunit heterogeneity: functional expression of cloned cDNAs. EMBO J. 8:1989;1665-1670
32. A/benzodiazepine receptors. EMBO J. 9:1990;3261-3267
33. Yu R., Follesa P., Ticku M.K. Down-regulation of the GABA receptor subunits mRNA levels in mammalian cultured cortical neurons following chronic neurosteroid treatment. Mol Brain Res. 41:1996;163-168
34. Zimmermann K., Mannhalter J.W. Technical aspects of quantitative competitive PCR. BioTechniques. 21:1996;268-272