Quantitation of messenger RNA by competitive RT-PCR: A simplified read out assay

Journal of Immunological Methods

Volumn 210, Issue 2, 1997, Pages 175-184

  Colle, Jean Hervé ^a   Falanga, Pierre B ^b   Singer, Monique ^a   Hevin, Brigitte ^a   Milon, Geneviève ^a  

^a INSTITUT PASTEUR   (France)

^b CNRS   (France)

Author keywords

Cytokines; Endothelial adhesion molecules; Inducible nitric oxide synthase; Mouse; mRNA; RT PCR quantitation

Indexed keywords

BETA ACTIN; CYTOKINE; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; GAMMA INTERFERON; INTERCELLULAR ADHESION MOLECULE 1; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 2; INTERLEUKIN 3; INTERLEUKIN 4; MESSENGER RNA; NITRIC OXIDE SYNTHASE; TRANSFORMING GROWTH FACTOR BETA1; VASCULAR CELL ADHESION MOLECULE 1;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; HOST RESISTANCE; IMMUNOREACTIVITY; LEISHMANIA MAJOR; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

ACTINS; ANIMALS; DNA; INTERFERON TYPE II; INTERLEUKIN-4; MICE; MICE, INBRED C57BL; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

ANIMALIA; LEISHMANIA MAJOR;

EID: 0031590667     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00186-5     Document Type: Article

References (28)

1. Alard P., Lantz O., Sebagh M., Calvo C.F., Weill D., Chavanel G., Senik A., Charpentier B. A versatile ELISA-PCR assay for mRNA quantitation from a few cells. Biotechniques. 15:1993;730-737.
2. Alonso S., Minty A., Bourlet Y., Buckingham M. Comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates. J. Mol. Evol. 23:1986;11-22.
3. Apostolakos M.J., Chuermann W.H.T., Frampton M.W., Utell M.J., Willey J.C. Measurement of gene expression by multiplex competitive polymerase chain reaction. Anal. Biochem. 213:1993;277-284.
4. Ballantyne C.M., O'Brien W.E., Beaudet A.L. Nucleotide sequence of the cDNA for murine intercellular adhesion molecule-1 (ICAM-1). Nuleic Acids Res. 17:1989;5853.
5. Becker-André M. Absolute levels of mRNA by polymerase chain reaction-aided transcript titration assay. Methods Enzymol. 218:1993;420-445.
6. Bolton C.M., Dudhia J., Bayliss M.T. Quantification of aggrecan and link-protein mRNA in human articular cartilage of different ages by competitive reverse transcriptase-PCR. Biochem. J. 319:1996;489-498.
7. Bouaboula M., Legoux P., Pessegue B., Delpech B., Dumont X., Piechaczyk M., Casellas P., Shire D. Standardization of mRNA titration using a polymerase chain reaction method involving co-amplification with a multispecific internal control. J. Biol. Chem. 267:1992;21830-21838.
8. Chirgwin J.M., Przybyla A.E., MacDonald R.J., Rutter W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 18:1979;5294-5299.
9. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
10. Derynck R., Jarrett J.A., Chen E.Y., Goeddel D.V. The murine transforming growth factor-beta precursor. J. Biol. Chem. 261:1986;4377-4379.
11. Gilliland G., Perrin S., Blanchard K., Bunn H.F. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA. 87:1990;2725-2729.
12. Gray P.W., Goeddel D.V. Cloning and expression of murine immune interferon cDNA. Proc. Nat. Acad. Sci. USA. 80:1983;5842-5846.
13. Griffais R., André P.M., Thibon M. K-tuple frequency in the human genome and polymerase chain reaction. Nucleic Acids Res. 19:1991;3887-3891.
14. Hession C., Moy P., Tizard R., Chisholm P., Williams C., Wysk M., Burkly L., Miyake K., Kincade P., Lobb R. Cloning of murine and rat vascular cell adhesion molecule-1. Biochem. Biophys. Res. Comm. 183:1992;163-169.
15. Holodniy, M., Winters, M.A., Merigan, T.C., 1992. Detection and quantification of gene amplification products by a nonisotopic automated system. Biotechniques, vol. 12, p. 36 and 38-39.
16. Kashima N., Nishi-Takaoka C., Fujita T., Taki S., Yamada G., Hamuro J., Taniguchi T. Unique structure of murine interleukin-2 as deduced from cloned cDNAs. Nature. 313:1985;402-404.
17. Kindler V., Thorens B., de Kossodo S., Alet B., Eliason J.F., Thatcher D., Farber N., Vassalli P. Stimulation of hematopoiesis in vivo by recombinant bacterial murine interleukin 3. Proc. Nat. Acad. Sci. USA. 83:1986;1001-1005.
18. Moore, K.W., Vieira, P., Fiorentino, D.F., Trounstine, M.L., Khan, T.A., Mosman, T.R., 1990. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI [published erratum appears in Science 1990 Oct. 26; 250(4980):494]: Science, v. 248, p. 1230-1234.
19. Moore, K.W., Vieira, P., Fiorentino, D.F., Trounstine, M.L., Khan, T.A., Mosman, T.R., 1990. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI [published erratum appears in Science 1990 Oct. 26; 250(4980):494]: Science, v. 248, p. 1230-1234.
20. Noma Y., Sideras P., Naito T., Bergstedt-Lindquist S., Azuma C., Severinson E., Tanabe T., Kinashi T., Matsuda F., Yaoita Y.et al. Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter. Nature. 319:1986;640-646.
21. Pannetier C., Delassus S., Darche S., Saucier C., Kourilsky P. Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation. Nucleic Acids Res. 21:1993;577-583.
22. Porcher C., Malinge M.C., Picat C., Grandchamp B. A simplified method for determination of specific DNA or RNA copy number using quantitative PCR and an automatic DNA sequencer. Biotechniques. 13:1992;106-114.
23. Reiner S.L., Zheng S., Corry D.B., Locksley R.M. Constructing polycompetitor cDNAs for quantitative PCR. J. Immunol. Methods. 165:1993;37-46. published errata appear in J. Immunol. Methods 1994 Jul 12; 173(1), 133 and 1994 Oct 14; 175(2), 275.
24. Schoenhaut D.S., Chua A.O., Wolitzky A.G., Quinn P.M., Dwyer C.M., McComas W., Familletti P.C., Gately M.K., Gubler U. Cloning and expression of murine IL-12. J. Immunol. 148:1992;3433-3440.
25. Singer-Sam J., Riggs A.D. Quantitative analysis of messenger RNA levels: reverse transcription-polymerase chain reaction single nucleotide primer extension assay. Methods Enzymol. 225:1993;344-351.
26. Stieger M., Démollière C., Ahlborn-Laake L., Mous J. Competitive polymerase chain reaction assay for quantitation of HIV-1 DNA and RNA. J. Virol. Methods. 34:1991;149-160.
27. Wang A.M., Doyle M.V., Mark D.F. Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA. 86:1989;9717-9721. published erratum appears in Proc. Natl. Acad. Sci. USA 1990 Apr; 87(7), 2865.
28. Xie Q.W., Cho H.J., Calaycay J., Mumford R.A., Swiderek K.M., Lee T.D., Ding A., Troso T., Nathan C. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 256:1992;225-228.