Trafficking of non-regulated secretory proteins in insulin secreting (INS-1) cells

Diabetologia

Volumn 43, Issue 9, 2000, Pages 1157-1164

  Molinete, M ^a   Lilla, V ^a   Jain, R ^b   Joyce, P B M ^c   Gorr, S U ^b   Ravazzola, M ^a   Halban, P A ^a  

^a UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^b UNIVERSITY OF LOUISVILLE SCHOOL OF MEDICINE   (United States)

^c Gina Cody School of Engineering and Computer Science   (Canada)

Author keywords

GFP; Insulin; Regulated secretory pathway; Secretory alkaline phosphatase; Secretory granules

Indexed keywords

ALKALINE PHOSPHATASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INSULIN; OLIGOMER; PROINSULIN; SECRETORY PROTEIN;

ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; ELECTRON MICROSCOPY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; INSULIN RELEASE; OLIGOMERIZATION; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SECRETION; SECRETORY GRANULE; WESTERN BLOTTING;

ADENOVIRIDAE; ALKALINE PHOSPHATASE; ANIMALS; CELL LINE; CYTOPLASMIC GRANULES; CYTOSOL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INSULIN; ISLETS OF LANGERHANS; KINETICS; LUMINESCENT PROTEINS; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0033817564     PISSN: 0012186X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001250051507     Document Type: Article

References (34)

1. Pathways of protein secretion in eukaryotes
2. Sorting and processing of secretory proteins
3. The trans-Golgi network: A late secretory sorting station
4. Sorting and storage during secretory granule biogenesis: Looking backward and looking forward
5. The transmost cisternae of the Golgi complex: A compartment for sorting of secretory and plasma membrane proteins
6. Distinct molecular mechanisms for protein sorting within immature secretory granules of pancreatic beta-cells
7. Sorting of a constitutive secretory protein to the regulated secretory pathway of exocrine cells
8. Newly synthesized proinsulin/insulin and stored insulin are released from pancreatic B cells predominantly via a regulated, rather than a constitutive, pathway
9. Establishment of 2-mercaptoethanol-dependent differentiated insulin- Secreting cell lines
10. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo
11. Slow cleavage at the proinsulin B-chain/connecting peptide junction associated with low levels of endoprotease PC1/3 in transformed β cells
12. Proinsulin processing in the rat insulinoma cell line INS after overexpression of the endoproteases PC2 or PC3 by recombinant adenovirus
13. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4
14. Coated charcoal immunoassay of insulin
15. Bidirectional transport by distinct populations of COPI-coated vesicles
16. Recognition of human insulin and proinsulin by monoclonal antibodies
17. The amino-terminal sequence of pro-opiomelanocortin directs intracellular targeting to the regulated secretory pathway
18. Chromogranin B (secretogranin I), a secretory protein of the regulated pathway, is also present in a tightly membrane-associated form in PC12 cells
19. Carboxypeptidase E is a regulated secretory pathway sorting receptor: Genetic obliteration leads to endocrine disorders in Cpe (fat) mice
20. Depletion of carboxypeptidase E, a regulated secretory pathway sorting receptor, causes misrouting and constitutive secretion of proinsulin and proenkephalin, but not chromogranin A
21. Protein secretion: Puzzling receptors
22. Proinsulin targeting to the regulated pathway is not impaired in carboxypeptidase E-deficient Cpefat/Cpefat mice
23. The rate of bulk flow from the endoplasmic reticulum to the cell surface
24. Secreted placental alkaline phosphatase: A powerful new quantitative indicator of gene expression in eukaryofic cells
25. Differential storage of prolactin, granins (chromogranin B and secretogranin II), and constitutive secretory markers in rat pituitary GH4C1 cells
26. Novel markers for constitutive secretion used to show that tissue plasminogen activator is sorted to the regulated pathway in transfected PC12 cells
27. Insulin targeting to the regulated secretory pathway after fusion with green fluorescent protein and firefly luciferase
28. Differential sorting of lysosomal enzymes out of the regulated secretory pathway in pancreatic beta-cells
29. Microtubule-dependent transport of secretory vesicles visualized in real time with a GFP-tagged secretory protein
30. Secretory-granule dynamics visualized in vivo with a phogrin-green fluorescent protein chimaera
31. Real-time imaging of the axonal transport of granules containing a tissue plasminogen activator/green fluorescent protein hybrid
32. Improved green fluorescence
33. Targeting of green fluorescent protein to neuroendocrine secretory granules: A new tool for real time studies of regulated protein secretion
34. The green fluorescent protein targets secretory proteins to the yeast vacuole