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Natori S, Huttner WB: Chromogranin B (secretogranin I) promotes sorting to the regulated secretory pathway of processing intermediates derived from a peptide hormone precursor. Proc Natl Acad Sci USA 1996, 93:4431-4436.
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Natori, S.1
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fat mice
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fat mice. Cell 1997, 88:73-83. An iodinated amino-terminal portion of pro-opiomelanocortin is used to identify a putative common regulated secretory protein sorting receptor. The radiolabeled probe is found to bind to carboxypeptidase E (CPE) and, using antisense RNA and CPE-knockout mouse models, evidence for the involvement of CPE in sorting is presented.
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(1997)
Cell
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Cool, D.R.1
Normant, E.2
Shen, F.3
Chen, H.4
Pannell, L.5
Zhang, Y.6
Loh, Y.P.7
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48
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Protein kinase C bound to the Golgi apparatus supports the formation of constitutive transport vesicles
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Westermann P, Knoblich M, Maier O, Lindschau C, Haller H: Protein kinase C bound to the Golgi apparatus supports the formation of constitutive transport vesicles. Biochem J 1996, 320:651-658.
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Lindschau, C.4
Haller, H.5
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49
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The production of post-Golgi vesicles requires a protein kinase C-like molecule, but not its phosphorylating activity
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•], it is shown that assembly of TGN-derived vesicles occurs in at least two distinct stages, one involving the assembly of a coat and a second comprising the vesicle scission reaction. The first, coat-assembly, stage occurs at 20°C; PKC inhibitors block the second, scission, stage but, interestingly, must be added during the first stage to be effective.
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J Cell Biol
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Simon, J.-P.1
Ivanov, I.E.2
Adesnik, M.3
Sabatini, D.D.4
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Formation of nascent secretory vesicles from the trans-Golgi network of endocrine cells is inhibited by tyrosine kinase and phosphatase inhibitors
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Austin CD, Shields D: Formation of nascent secretory vesicles from the trans-Golgi network of endocrine cells is inhibited by tyrosine kinase and phosphatase inhibitors. J Cell Biol 1996, 135:1471-1483. Addition of inhibitors of either tyrosine kinase or tyrosine phosphatase activity inhibits the budding of polypeptide-hormone-containing nascent secretory vesicles from the TGN. Using anti-phosphotyrosine antibodies, the phosphorylation of several membrane-associated proteins is indirectly correlated with the inhibition of vesicular transport.
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J Cell Biol
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Austin, C.D.1
Shields, D.2
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