Depletion of carboxypeptidase E, a regulated secretory pathway sorting receptor, causes misrouting and constitutive secretion of proinsulin and proenkephalin, but not chromogranin A

Endocrinology

Volumn 139, Issue 4, 1998, Pages 2137-2145

  Normant, Emmanuel ^a   Loh, Y Peng ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYPEPTIDASE H; CHROMOGRANIN A; COMPLEMENTARY RNA; PROENKEPHALIN; PROINSULIN;

ARTICLE; ELECTRON MICROSCOPY; NEUROSECRETORY CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN TARGETING;

EID: 0031764282     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.139.4.5951     Document Type: Article

References (39)

1. Gumbiner B, Kelly RB 1982 Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28:51-59
2. Kelly RB 1985 Pathways of protein secretion in eukaryotes. Science 230:25-32
3. Chanat E, Huttner WB1991 Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network. J Cell Biol 115:1505-1519
4. ++-dependent aggregation property of secretory vesicle matrix proteins and the potential role of chromogranins A and B in secretory vesicles biogenesis. J Biol Chem 271:1558-1565
5. Yoo SH 1995 pH- and Ca(2+)-induced conformational change and aggregation of chromogranin B. Comparison with chromogranin A and implication. J Biol Chem 270:12578-12583
6. Gerdes H-H, Rosa P, Phillips E, Baeuerle PA, Frank R, Argos P, Huttner WB 1989 The primary structure of human secretogranin II, a widespread tyrosine-sulfated secretory granule protein that exhibits low pH- and calcium-induced aggregation. J Biol Chem 264:12009-12015
7. Song L, Fricker LD 1995 Calcium- and pH-dependent aggregation of carboxypeptidase E. J Biol Chem 270:7963-7967
8. Shennan KI, Taylor NA, Docherty K 1994 Calcium- and pH-dependent aggregation and membrane association of the precursor of the prohormone convertase PC2. J Biol Chem 269:18646-18650
9. Colomer V, Kicska GA, Rindler MJ 1996 Secretory granule content proteins and the lumenal domains of granules membrane proteins aggregate in vitro at mildly acidic pH. J Biol Chem 271:48-55
10. Seksek O, Biwersi J, Verkman AS 1995 Direct measurement of trans-Golgi pH in living cells and regulation by second messengers. J Biol Chem 270:4967-4970
11. Kim JH, Lingwood CA, Williams DB, Furuya W, Manolson MF, Grinstein S 1996 Dynamic measurement of the pH of the Golgi complex in living cells using retrograde transport of the verotoxin receptor. J Cell Biol 134:1387-1399
12. Chandra S, Cable EPW, Morrison GH, Webb WW 1991 Calcium sequestration in the Golgi apparatus of cultured mammalian cells revealed by laser scanning confocal microscopy and ion microscopy. J Cell Sci 10:747-752
13. Chanat E, Weiss U, Huttner W B 1994 The disulfide bond in chromogranin B, which is essential for its sorting to secretory granules, is not required for its aggregation in the trans Golgi network. FEBS Lett 351:225-230
14. Schmidt WK, Moore H-P 1994 Synthesis and targeting of insulin-like growth factor-I to the hormone storage granules in an endocrine cell line. J Biol Chem 269:27115-27124
15. Tam WHH, Andreasson KA, Loh YP 1993 The amino-terminal sequence of pro-opiomelanocortin directs intracellular targeting to the regulated secretory pathway. Eur J Cell Biol 62:294-306
16. Creemers JWM, Vsac EF, Bright NA, Van De Loo J-W, Jansen E, Van De Ven WJM, Hutton JC 1996 Identification of a transferable sorting domain for the regulated pathway in the prohormone convertase PC2. J Biol Chem 271:25284-25291
17. Moore H-PH, Kelly RB 1986 Re-routing of a secretory protein fusion with human growth hormone sequences. Nature 321:443-446
18. Stoller TJ, Shields D 1989 The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells. J Cell Biol 108:1647-1655
19. Sevarino KA, Stork P, Ventimiglia R, Mandel G, Goodman RH 1989 Aminoterminal sequences of prosomatostatin direct intracellular targeting but not processing specificity. Cell 57:11-19
20. Parmer RJ, Xi X-P, Wu H-J, Helman LJ, Petz LN 1993 Secretory protein traffic. Chromogranin A contains a dominant targeting signal for the regulated pathway. J Clin Invest 92:1042-1054
21. Cool DR, Fenger M, Snell CR, Loh PY 1995 Identification of the sorting signal motif within pro-opiomelanocortin for the regulated secretory pathway. J Biol Chem 270:8723-8729
22. Chanat E, Weiss U, Huttner WB, Tooze SA 1993 Reduction of the disulfide bond of chromogranin B (secretogranin I) in the trans-Golgi network causes its missorting to the constitutive secretory pathway. EMBO J 12:2159-2168
23. Rosa P, Weiss U, Pepperkok R, Ansorge W, Niehrs C, Stelzer EH, Huttner WB 1989 An antibody against secretogranin I (chromogranin B) is packaged into secretory granules. J Cell Biol 109:17-34
24. Kang YK, Yoo SH 1997 Identification of the secretory vesicle membrane binding region of chromogranin A. FEBS Lett 404:87-90
25. De Bie I, Marcinkiewicz M, Malide D, Lazure C, Nakayama K, Bendayan M, Seidah NG 1996 The isoforms of proprotein convertase PC5 are sorted to different subcellular compartments. J Cell Biol 135:1261-1275
26. Milgram SL, Mains RE, Eipper BA1996 Identification of routing determinants in the cytosolic domain of a secretory granule-associated integral membrane protein. J Biol Chem 271:17526-17535
27. fat mice. Cell 88:73-83
28. Fricker LD, Das B, Angeletti RH 1990 Identification of the pH-dependent membrane anchor of carboxypeptidase E(EC 3.4.17.10). J Biol Chem 265:2476-2482
29. Sabol SL, Liang C-M, Dandekar S, Kranzel LS 1983 In vitro biosynthesis and processing of immunologically identified methionin-enkephalin precursor protein. J Biol Chem 258:2697-2704
30. Bamberger A-M, Pu L-P, Cool DR, Loh YP 1995 The Neuro-2a neuroblastoma cell line expresses [Met]-enkephalin and vasopressin mRNA and peptide. Mol Cell Endocrinol 113:155-163
31. fat mice. J Biol Chem 272:27532-27534
32. Naggert JK, Fricker LD, Varlamov O, Nishina PM, Rouillé Y, Steiner DF, Carroll RJ, Paigen BJ, Leiter EH 1995 Hyperproinsulinemia in obese fat/fat mice associated with a Carboxypeptidase E mutation which reduces enzyme activity. Nat Genet 10:135-142
33. fat mice are defective in Carboxypeptidase E and proinsulin processing. Endocrinology 138:4883-4892
34. Seidah NG, Chretien M, Day R 1994 The family of subtilisin/kexin like pro-protein and prohormone convertases: divergent or shared functions. Biochimie 76:197-209
35. Brechler V, Chu WN, BaxterJD, Thibault G, Reudelhuber TL 1996 A protease processing site is essential for protein sorting to the regulated pathway. J Biol Chem 271:20636-20640
36. Jung LJ, KreinerT, Scheller RH 1993 Expression of mutant ELH prohormones in AtT-20 cells: the relationship between prohormone processing and sorting. J Cell Biol 121:11-21
37. Tooze SA, Chanat E, Tooze J, Huttner WB 1993 Secretory granule formation. In: Loh YP (ed) Mechanism of Intracellular Trafficking and Processing of Proproteins. CRC Press, Boca Raton, FL, pp 158-174
38. Moore H-PH, Walker ML, Lee F, Kelly RB 1983 Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35:531-538
39. fat mouse associated with carboxypeptidase E mutation. Proc Natl Acad Sci USA 94:5314-5319