Development and demonstration of RNA isolation and RT-PCR procedures to detect Escherichia coli O157:H7 gene expression on beef carcass surfaces

Letters in Applied Microbiology

Volumn 31, Issue 4, 2000, Pages 265-269

  Berry, E D ^a  

^a U S MEAT ANIMAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BEEF; PATHOGENS; POLYMERASE CHAIN REACTION; RNA; TRANSCRIPTION;

ESCHERICHIA COLI O157:H7; GENES EXPRESSION; GENETIC MECHANISM; GREEN FLUORESCENT PROTEIN; PATHOGEN RESISTANCE; PRESERVATION TECHNIQUES; PROCESSING TECHNIQUE; PROTEIN GENES; REVERSE TRANSCRIPTION PCR; RNA ISOLATION;

ESCHERICHIA COLI;

BACTERIAL RNA; GREEN FLUORESCENT PROTEIN;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; CARCASS; COW; ESCHERICHIA COLI; FOOD PRESERVATION; FOOD PROCESSING; FOOD SAFETY; GENE EXPRESSION; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; STRESS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033793106     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.2000.00803.x     Document Type: Article

References (28)

1. A competitive microflora increases the resistance of Salmonella typhimurium to inimical processes: Evidence for a suicide response
2. Preservation microbiology and safety: Evidence that stress enhances virulence and triggers adaptive mutations
3. Starvation- and stationary-phase-induced acid tolerance in Escherichia coli O157:H7
4. Thermotolerance of Listeria monocytogenes and Salmonella typhimurium after sublethal heat shock
5. RpoS regulation of acid, heat, and salt tolerance in Escherichia coli O157:H7
6. Improved green fluorescent protein by molecular evolution using DNA shuffling
7. Effect of prior heat shock on heat resistance of Listeria monocytogenes in meat
8. Primer design for a prokaryotic differential display RT-PCR
9. Optimization of differential display of prokaryotic mRNA: Application to pure culture and soil microcosms
10. Acid adaptation of Listeria monocytogenes can enhance survival in acidic foods and during milk fermentation
11. Tight regulation, modulation, and high-level expression by vectors containing the arabinose P(BAD) promoter
12. Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary phase thermotolerance in Escherichia coli
13. Preservation microbiology and safety: Quo vadis?
14. Acid adaptation promotes survival of Salmonella spp. in cheese
15. Acid adaptation induces cross-protection against environmental stresses in Salmonella typhimurium
16. Acid adaptation of Escherichia coli O157:H7 increases survival in acidic foods
17. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
18. Elevation of the heat resistance of Salmonella typhimurium by sublethal heat shock
19. Changes in the heat resistance of Salmonella typhimurium during heating at rising temperatures
20. The putative σ factor KatF has a central role in the development of starvation-mediated general resistance in Escherichia coli
21. Environmental signals controlling expression of virulence determinants in bacteria
22. In vitro nucleic acid amplification techniques
23. The significance of bacteria in stationary phase to food microbiology
24. Factors affecting the emergence of pathogens on foods
25. RpoS function in Salmonella Typhimurium LT2 monitored in a skim milk model food
26. Heat shock response enhances acid tolerance of Escherichia coli O157:H7
27. Stress-inducible gene of Salmonella typhimurium identified by arbitrarily primed PCR of RNA