Properties of ras-amplified recombinant BHK-21 cells in protein-free culture

Cytotechnology

Volumn 33, Issue 1-3, 2000, Pages 21-26

  Inoue, Yuichi ^a   Kawamoto, Seiji ^b   Shoji, Masahiro ^c   Hashizume, Shuichi ^c   Teruya, Kiichiro ^d   Katakura, Yoshinori ^d   Shirahata, Sanetaka ^d  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c 2 1 1 Shimosueyoshi   (Japan)

^d KYUSHU UNIVERSITY   (Japan)

Author keywords

BHK 21 cells; Human monoclonal antibody; Ras oncogene; Synthetic attachment factor

Indexed keywords

ANTIBODY PRODUCTION; CANCER CELL; CELL GROWTH; CELL LINE; CULTURE MEDIUM; GROWTH RATE; LUNG; MONOCLONAL ANTIBODY;

EID: 0033788164     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008163312841     Document Type: Conference Paper

References (20)

1. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker
2. High expression in mammalian cells without amplification
3. The regulated expression of erythropoietin by two human hepatoma cell lines
4. Production of recombinant human monoclonal antibody using ras-amplified BHK-21 cells in a protein-free medium
5. Production of a recombinant human monoclonal antibody using a novel hollow fiber bioreactor system
6. Long-term stable production of monocyte-colony inhibitor factor (M-CIF) from CHO microcarrier perfusion cultures
7. Malignant transformation of murine fibroblasts by a human c-Ha-ras-1 oncogene does not require a functional epidermal growth factor receptor
8. Spermine enhances IgM productivity of human-human hybridoma HB4C5 cells and human peripheral blood lymphocytes
9. Lysozyme stimulates immunoglobulin production by human-human hybridoma and human peripheral blood lymphocytes
10. Human-human hybridomas secreting antibodies specific to lung caminoma
11. Development of optimized transfectoma cell lines for production of chimeric antibodies in hollow fiber culture system
12. Ras and neu oncogenes reverse serum inhibition and epidermal growth factor dependence of serum-free mouse embryo cells
13. Lung cancer-reacting human recombinant antibody AE6F4: Potential usefulness in the sputum cytodiagnosis
14. The 14-13 protein as the antigen for lung cance-associated human monoclonal antibody AE6F4
15. Autocrine growth factors and cancer
16. Growth rate suppression of cultured mammlian cells enhances protein productivities
17. Ras amplification in BHK-21 cells produces a host cell line for further rapid establishment of recombinant protein hyper-producing cell lines
18. Pilot production of high-titer interferon and virus with a microcarrier suspension culture system
19. Changes in monoclonal antibody productivity of recombinant BHK cells immobilized in collagen gel particles
20. Ras oncogene enhances the production of a recombinant protein regulated by the cytomegalovirus promoter in BHK-21 cells