Spermine enhances IgM productivity of human-human hybridoma HB4C5 cells and human peripheral blood lymphocytes

Cytotechnology

Volumn 26, Issue 2, 1998, Pages 111-118

  Miyazaki, Yoshiyuki ^a   Nishimoto, Sougo ^a   Sasaki, Takeshi ^a   Sugahara, Takuya ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Human peripheral blood lymphocyte; Human human hybridoma; Immunoglobulin production stimulating factor; Serum free culture; Spermine

Indexed keywords

EID: 0031889504     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007939213564     Document Type: Article

References (15)

1. Fillingame RH, Jorstad CM and Morris DR (1975) Increased cellular levels of spermidine or spermine are required for optimal DNA synthesis in lymphocytes activated by concanavalin A. Proc. Nat. Acad. Sci. U.S.A. 72: 4042-4045.
2. Flink I and Pettijohn DE (1975) Polyamines stabilize DNA folds. Nature 253: 62-63.
3. Igarashi K, Aoki Y and Hiroshe S (1977) Base specificity of polyamine binding to synthetic polynucleotides. J. Biochem. 81: 1091-1096.
4. Ikemura T (1969) The relation between the strong binding of spermineto polynucleotides and the conformation of polynucleotides. Biochim, Biophys. Acta 195: 389-395.
5. Merten O-W (1988) Batch production and growth kinetics of hybridomas. Cytotechnology 1: 113-121.
6. Murakami F, Sasaki T and Sugahara T (1997) Lysozyme stimulates immunoglobulin production by human-human hybridoma and human peripheral blood lymphocytes. Cytotechnology 24: 177-182.
7. Murakami H, Masui H, Sato GH, Sueoka N, Chow TP and Kono T (1982) Growth of hybridoma cells in serum-free medium. Proc. Natl. Acad. Sci. U.S.A. 79: 1158-1162.
8. Murakami H, Hashizume S, Ohashi H, Shinohara K, Yasumoto K, Nomoto K and Omura H (1985) Human-human hybridomas secreting antibodies specific to human lung carcinoma. In Vitro Cell. Develop. Biol. 21: 593-596.
9. Pegg AE and McCann PP (1982) Polyamine metabolism and function. Cell Physiol. 12: 212-221.
10. Pingoud A, Urbanke C, Alves J, Ehbrecht H-J, Zabeau M and Gualerzi C (1984) Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases. Biochemistry 23: 5697-5703.
11. Sugahara T, Shirahata S, Akiyoshi K, Isobe T, Okuyama T and Murakami H (1991) Immunoglobulin production stimulating factor-IIα (IPSF-IIα) is glyceraldehyde-3-phosphate dehydrogenase like protein. Cytotechnology 6: 115-120.
12. Sugahara T, Nakajima H, Shirahata S and Murakami H (1992) Purification and characterization of immunoglobulin production stimulating factor-IIβ derived from Namalwa cells. Cytotechnology 10: 137-146.
13. Sugahara T, Sasaki T and Murakami H (1994) Enhancement of immunoglobulin productivity of human-human hybridoma HB4C5 cells by basic proteins and poly-basic amino acids. Biosci. Biotech. Biochem. 58: 2212-2214.
14. Sugahara T, Shirahata S, Sasaki T and Murakami H (1995) The mode of actions of glyceraldehyde-3-phosphate dehydrogenase identified as an immunoglobulin production stimulating factor. FEBS Lett. 368: 92-96.
15. Tabor CW and Tabor H (1976) 1,4-diaminobutane (putrescine), spermidine, and spermine. Annu. Rev. Biochem. 45: 285-306.