Rapid-cycle PCR in temporarily compartmentalized capillaries: Two-round PCR in a single capillary prevents product carry-over

BioTechniques

Volumn 29, Issue 4, 2000, Pages 680-684

  Berg, J ^a   Nagl, V ^a   Muhlbauer, G ^a   Stekel, H ^a  

^a KEPLER UNIVERSITY HOSPITAL   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GLASS; SILICONE OIL;

AGAR GEL ELECTROPHORESIS; ANALYTICAL EQUIPMENT; CAPILLARY; CHROMOSOME TRANSLOCATION; FLUORESCENCE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; HYBRIDIZATION; LYMPHOMA; MELTING POINT; POLYMERASE CHAIN REACTION; REACTION TIME; SHORT SURVEY;

POLYMERASE CHAIN REACTION;

EID: 0033782254     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Short Survey

References (9)

1. Rapid simultaneous amplification and detection of the MBR/JH chromosomal translocation by fluorescence melting curve analysis
2. All advanced stage non-Hodgkin's lymphomas with a polymerase chain reaction amplifiable breakpoint of bcl-2 have residual cells containing the bcl-2 rearrangement at evaluation and after treatment
3. Detection of cells bearing the t(14;18) translocation following myeloablative treatment and autologous bone marrow transplantation for follicular lymphoma
4. A new non-Hodgkin's B-cell line (DoHH2) with a chromosomal translocation t(14;18)(q32;q21)
5. Quantification of low-copy transcripts by continuous SYBR® green I monitoring during amplification
6. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
7. Prevention of product carry-over by single tube two-round (ST-2R) PCR: Application to BCR-ABL analysis in chronic myelogenous leukemia
8. The LightCycler(TM): A microvolume multisample fluorimeter with rapid temperature control
9. Nested primer PCR detection limits of HIV-1 in the background of increasing numbers of lysed cells