Characterization of the NAD+ binding site of Candida boidinii formate dehydrogenase by affinity labelling and site-directed mutagenesis

European Journal of Biochemistry

Volumn 267, Issue 22, 2000, Pages 6657-6664

  Labrou, Nikolas E ^a   Rigden, Danyel J ^b   Clonis, Yannis D ^a  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

Author keywords

Affinity labelling; Formate dehydrogenase; Molecular modelling

Indexed keywords

ADENOSINE DIPHOSPHATE; ALANINE; BACTERIAL ENZYME; FORMATE DEHYDROGENASE; LYSINE; MERCAPTOETHANOL; NICOTINAMIDE ADENINE DINUCLEOTIDE; NUCLEOTIDE; PEPTIDE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; SCHIFF BASE; SODIUM BOROHYDRIDE; TRYPSIN;

AFFINITY LABELING; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CANDIDA BOIDINII; COMPLEX FORMATION; ENZYME DEGRADATION; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME REACTIVATION; ENZYME SPECIFICITY; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SITE DIRECTED MUTAGENESIS;

ADENOSINE DIPHOSPHATE; AFFINITY LABELS; AMINO ACID SEQUENCE; BINDING SITES; CANDIDA; FORMATE DEHYDROGENASES; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NAD; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN SUBUNITS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); CANDIDA BOIDINII; FELIS CATUS; PSEUDOMONAS;

EID: 0033769472     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01761.x     Document Type: Article

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