Regulation of the formate dehydrogenase gene, FDH1, in the methylotrophic yeast Candida boidinii and growth characteristics of an FDH1- disrupted strain on methanol, methylamine, and choline

Journal of Bacteriology

Volumn 179, Issue 14, 1997, Pages 4480-4485

  Sakai, Yasuyoshi ^a   Murdanoto, Agung Primanto ^a   Konishi, Tohru ^a   Iwamatsu, Akihiro ^b   Kato, Nobuo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KIRIN BREWERY CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLINE; FORMATE DEHYDROGENASE; GLUCOSE; METHANOL; METHYLAMINE;

ARTICLE; CANDIDA BOIDINII; ENZYME REGULATION; FUNGUS CULTURE; FUNGUS MUTATION; GENE DISRUPTION; GROWTH REGULATION; PRIORITY JOURNAL; STRAIN DIFFERENCE;

CANDIDA BOIDINII; FUNGI;

EID: 0030762532     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.14.4480-4485.1997     Document Type: Article

References (29)

1. Allen, S. J., and J. J. Holbrook. 1995. Isolation, sequence and overexpression of the gene encoding NAD-dependent formate dehydrogenase from the methylotrophic yeast Candida methylica. Gene 162:99-104.
2. Anthony, C. 1982. Biochemistry of methylotrophs. Academic Press, London.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Bystrykh, L. V., L. R. Aminova, and Y. A. Trotsenko. 1988. Methanol metabolism in mutants of the methylotrophic yeast, Hansenula polymorpha. FEMS Microbiol. Lett. 51:89-94.
5. Church, G. M., and W. Gilbert. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81:1991-1995.
6. Cregg, J. M. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology 11:905-910.
7. Cryer, D. R., R. Eccleshal, and J. Murmur. 1975. Isolation of yeast DNA. Methods Cell Biol. 12:39-44.
8. Davis, R. W., M. Thomas, J. Cameron, T. P. S. John, S. Scherer, and R. A. Padgett. 1980. Rapid DNA isolation tor enzymatic and hybridization analysis. Methods Enzymol. 65:404-411.
9. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
10. Gellissen, G., Z. A. Janowicz, A. Merckelbach, M. Piontek, P. Keup, U. Weydemann, and C. P. Hollenberg. 1991. Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase. Bio/Technology 9:291-295.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
12. Lang, E., and H. Lang. 1972. Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure. Z. Anal. Chem. 260:8-10.
13. Mori, N., K. Shirakawa, K.-S. Uzura, Y. Kitamoto, and Y. Ichikawa. 1988. Formation of ethylene glycol and trimethylamine from choline by Candida tropicalis. FEMS Microbiol. Lett. 51:41-44.
14. Murdanoto, A. P., Y. Sakai, T. Konishi, F. Yasuda, Y. Tani, and N. Kato. 1997. Purification and properties of methyl formate synthase, a mitochondrial alcohol dehydrogenase, participating in formaldehyde oxidation in methylotrophic yeasts. Appl. Environ. Microbiol. 63:1715-1720.
15. Nash, T. 1953. The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem. J. 55:416-421.
16. Sakai, Y. Unpublished data.
17. Sakai, Y., M. Akiyama, H. Kondoh, Y. Shibano, and N. Kato. 1996. High-level secretion of fungal glucoamylase using the Candida boidinii gene expression system. Biochim. Biophys. Acta 1308:81-87.
18. Sakai, Y., T. K. Goh, and Y. Tani. 1993. High-frequency transformation of a methylotrophic yeast, Candida boidinii, with autonomously replicating plasmids which are also functional in Saccharomyces cerevisiae. J. Bacteriol. 175:3556-3562.
19. Sakai, Y., T. Kazarimoto, and Y. Tani. 1991. Transformation system for an asporogenous methylotrophic yeast, Candida boidinii: cloning of the orotidine-5′-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation. J. Bacteriol. 173:7458-7463.
20. Sakai, Y., A. P. Murdanoto, L. Sembiring, Y. Tani, and N. Kato. 1995. A novel formaldehyde oxidation pathway in methylotrophic yeasts: methylformate as a possible intermediate. FEMS Microbiol. Lett. 127:229-234.
21. Sakai, Y., T. Rogi, R. Takeuchi, N. Kato, and Y. Tani. 1995. Expression of Saccharomyces adenylate kinase gene in Candida boidinii under the regulation of its alcohol oxidase promoter. Appl. Microbiol. Biotechnol. 42:860-864.
22. Sakai, Y., T. Rogi, T. Yonehara, N. Kato, and Y. Tani. 1994. High-level ATP production by a genetically-engineered Candida yeast. Biotechnology 12: 291-293.
23. Sakai, Y., A. Saiganji, H. Yurimoto, K. Takabe, H. Saiki, and N. Kato. 1996. The absence of Pmp47, a putative yeast peroxisomal transporter, causes a defect in transport and folding of a specific matrix enzyme. J. Cell Biol. 134:37-51.
24. Sakai, Y., and Y. Tani. 1992. Directed mutagenesis in an asporogenous methylotrophic yeast: cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains. J. Bacteriol. 174:5988-5993.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Schütte, H., J. Flossdorf, H. Sahm, and M.-R. Kula. 1976. Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii. Eur. J. Biochem. 62:151-160.
27. 2 are non-essential for energy supply of yeast methylotrophic growth. Arch. Microbiol. 154:566-575.
28. Subramani, S. 1993. Protein import into peroxisomes and biogenesis of the organelle. Annu. Rev. Cell Biol. 9:445-478.
29. Tani, Y., Y. Sakai, and H. Yamada. 1985. Isolation and characterization of a mutant of a methanol yeast, Candida boidinii S2, with higher formaldehyde productivity. Agric. Biol. Chem. 49:2699-2706.