The Haemophilus influenzae Hia adhesin is an autotransporter protein that remains uncleaved at the C terminus and fully cell associated

Journal of Bacteriology

Volumn 182, Issue 21, 2000, Pages 6005-6013

  St Geme III, J W ^a   Cutter, D ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; AMINO ACID; CARRIER PROTEIN; HYBRID PROTEIN; PROTEIN HIA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL INFECTION; BACTERIUM ADHERENCE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; NASOPHARYNX; NONHUMAN; OUTER MEMBRANE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RESPIRATORY EPITHELIUM; RESPIRATORY TRACT DISEASE; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

ADHESINS, BACTERIAL; ALKALINE PHOSPHATASE; ANIMALS; ARTIFICIAL GENE FUSION; BACTERIAL ADHESION; BACTERIAL OUTER MEMBRANE PROTEINS; CELL LINE; CHIMERA; CYCLIN-DEPENDENT KINASES; ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; IMMUNOHISTOCHEMISTRY; MUTAGENESIS, SITE-DIRECTED; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; NEGIBACTERIA;

EID: 0033765760     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.21.6005-6013.2000     Document Type: Article

References (58)

1. Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae high-molecular-weight surface-exposed proteins related to filamentous hemagglutinin of Bordetella pertussis
2. Genes encoding high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae are part of gene clusters
3. Identification of a second family of high-molecular-weight adhesion proteins expressed by non-typable Haemophilus influenzae
4. SepA, the major extracellular protein of Shigella flexneri: Autonomous secretion and involvement in tissue invasion
5. Cloning and expression of an adhesin (AIDA-I) involved in diffuse adherence of enteropathogenic Escherichia coli
6. EspP, a novel extracellular serine protease of enterohemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V
7. Rapid procedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species
8. Construction and characterization of amplifiable mutticopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
9. Prediction of β-turns
10. Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products
11. SopA, the outer membrane protease responsible for polar localization of IcsA in Shigella flexneri
12. Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli
13. A plasmid facilitating in vitro construction of phoA gene fusions in Escherichia coli
14. Characterization of Pic, a secreted protease of Shigella flexneri and enteroaggregative Escherichia coli
15. The great escape: Structure and function of the autotransporter proteins
16. The major phase-variable outer membrane protein of Escherichia coli structurally resembles the immunoglobulin A1 protease class of exported protein and is regulated by a novel mechanism involving Dam and OxyR
17. Structural determinants of processing and secretion of the Haemophilus influenzae Hap protein
18. The Haemophilus influenzae Hap serine protease promotes adherence and microcolony formation, potentiated by a soluble host protein
19. Outer membrane proteins of Escherichia coli: Mechanism of sorting and regulation of synthesis
20. Defined non-growth media for stage II development of competence in Haemophilus influenzae
21. Recombinant PCR
22. Fusions of secreted proteins to alkaline phosphatase: An approach for studying protein secretion
23. Type III protein secretion systems in bacterial pathogens of animals and plants
24. Common structural features of IgA1 protease-like outer membrane protein autotransporters
25. Characterization of the Neisseria Iga beta-core: The essential unit for outer membrane targeting and extracellular protein secretion
26. Extracellular transport of cholera toxin B subunit using Neisseria IgA protease beta-domain: Conformation-dependent outer membrane translocation
27. Selective extracellular release of cholera toxin B subunit by Escherichia coli: Dissection of Neisseria Iga(β)-mediated outer membrane transport
28. Adhesin expression in matched nasopharyngeal and middle ear isolates of nontypeable Haemophilus influenzae from children with acute otitis media
29. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
30. Identification of a glycoprotein produced by enterotoxigenic Escherichia coli
31. Analysis of membrane protein topology using alkaline phosphatase and β-galactosidase gene fusions
32. TnphoA: A transposon probe for protein export signals
33. Characterization of the essential transport function of the AIDA-I autotransporter and evidence supporting structural predictions
34. Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypeable Haemophilus influenzae: Pathogenic and epidemiologic observations
35. Mechanism of assembly of the outer membrane of Salmonella typhimurium: Isolation and characterization of cytoplasmic and outer membrane
36. Identification and molecular characterization of a major ring-forming surface protein from the gastric pathogen Helicobacter mustelae
37. Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease
38. Filamentous hemagglutinin of Bordetella pertussis: Nucleotide sequence and crucial role in adherence
39. Prediction of protein secondary structure at better than 70% accuracy
40. Prediction of membrane-spanning β-strands and its application to maltoporin
41. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid
42. The C-terminal domain of the Bordetella pertussis autotransporter BrkA forms a pore in lipid bilayer membranes
43. Secretion of virulence determinants by the general secretory pathway in gram-negative pathogens: An evolving story
44. Characterization of the avian pathogenic Escherichia coli hemagglutinin Tsh, a member of the immunoglobulin A protease-type family of autotransporters
45. Characterization of EspC, a 110-kilodalton protein secreted by enteropathogenic Escherichia coli which is homologous to members of the immunoglobulin A protease-like family of secreted proteins
46. Insights into the mechanism of respiratory tract colonization by nontypable Haemophilus influenzae
47. A Haemophilus influenzae IgA protease-like protein promotes intimate interaction with human epithelial cells
48. Haemophilus influenzae adheres to and enters cultured human epithelial cells
49. High-molecular-weight proteins of nontypable Haemophitus influenzae mediate attachment to human epithelial cells
50. Secretion of the Haemophilus influenzae HMW1 and HMW2 adhesins involves a periplasmic intermediate and requires the HMWB and HMWC proteins
51. Prevalence and distribution of the hmw and hia genes and the HMW and Hia adhesins among genetically diverse strains of nontypeable Haemophilus influenzae
52. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
53. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
54. Transposon mutagenesis, characterization, and cloning of transformation genes of Haemophilus influenzae Rd.
55. The pathogenicity of Haemophilus influenzae
56. Probing secretion and translocation of a β-autotransporter using a reporter single-chain Fv as a cognate passenger domain
57. A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa