Oxidation of cellular thiols by hydroxyethyldisulphide inhibits DNA double-strand-break rejoining in G6PD deficient mammalian cells

International Journal of Radiation Biology

Volumn 76, Issue 11, 2000, Pages 1523-1531

  Ayene, I S ^a   Koch, C J ^a   Tuttle, S W ^a   Stamato, T D ^a   Perez, M L ^a   Biaglow, J E ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DISULFIDE; DOUBLE STRANDED DNA; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; THIOL DERIVATIVE;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CLONOGENIC ASSAY; CONTROLLED STUDY; DNA DETERMINATION; DNA STRAND BREAKAGE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE DEFICIENCY; IONIZING RADIATION; MAMMAL; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS;

EID: 0033756418     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/09553000050176289     Document Type: Article

References (43)

1. Redox regulation of fos and Jun DNA-binding activity in vitro
2. Bridge over troubled waters: Sensing stress by disulfide bond formation
3. DNA as an important target in radiation induced apoptosis of myc and myc plus ras transfected rat embryo fibroblasts
4. Role of scavenger derived radicals in the induction of doublestrand and single strand breaks in irradiated DNA
5. Simulation of the cellular oxygen effect with an in vitro model system using DNA strand breaks as an endpoint
6. Diamide induced shift in protein and glutathione thiol: Disulfide status delays DNA rejoining X-irradiation of human cancer cells
7. A method for measuring disulfide reduction by cultured mammalian cells: Relative contributions of GSH dependent and independent mechanisms
8. The importance of peroxide and superoxide in the X-ray response
9. Selenium and the thioredoxin and glutaredoxin systems
10. Diamide-induced cytotoxicity and thermotolerance in CHO cells
11. The inactivation of chinese hamster cells by X rays: Synchronized and exponential cell populations
12. The inactivation of chinese hamster cells by X rays: The effects of chemical modifiers on single and double-events
13. Identification of redox-sensitive cysteines in GA-binding protein-alpha that regulate DNA binding and heterodimerization
14. Different mechanisms of formation of glutathione-protein mixed disulfides of diamide and tert-butyl hydroperoxide in rat blood
15. Cell cycle dependent repair of double strand DNA breaks in a gamma ray sensitive chinese hamster cell line
16. Implication of DNA-dependent protein kinase in an early, essential, local phosphorylation event during end-joining of DNA double-strand breaks in vitro
17. Role of the pentose cycle in oxygen radical-mediated toxicity of the thiol-containing radioprotector dithiothreitol in mammalian cells
18. The influence of glutathione depleting agents on human platelet function
19. Analysis of tumor thiol concentrations: Comparison of flow cytometric with chemical and biochemical techniques
20. The role of DNA double-strand breaks in ionizing radiation-induced killing of eukaryotic cells
21. X-ray sensitive mutants of chinese hamster ovary cell lines: Isolation and cross-sensitivity to other DNA-damaging agents
22. Tert-butylhydroperoxide-induced toxicity, in isolated hepatocytes: Contribution of thiol oxidation and lipid peroxidation
23. X-ray sensitive mutants of CHO cells defective in double-strand break rejoining
24. ATP-dependent transport of glutathione-N-ethyl-maleimideconjugate across erythrocyte membrane
25. The mechanism of radioprotection by nonprotein sulphydryls: Cysteine, glutathione and cysteamine
26. Mechanisms of light modulation: Identification of potential redox-sensitive cysteines distal to catalytic site in light-activated chloroplast enzymes
27. The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination
28. Conserved cysteine residue in the DNA-binding domain of the bovine papillomavirus type 1 E2 protein confers redox regulation of the DNA-binding activity in vitro
29. Thioltransferase in human red blood cells: Kinetics and equilibrium
30. A redox-dependent function of thioredoxin is necessary to sustain a rapid rate of DNA synthesis in yeast
31. Engineering a domainlocking disulfide into a bacterial malate dehydrogenase produces a redox-sensitive enzyme
32. Redox regulation of ubiquitinconjugating enzymes: Mechanistic insights using the thiol-specific oxidant diamide
33. Casein kinase 2 binds to and phosphorylates BRCA1
34. Redox signalling and the control of cell growth and death
35. Nuclear magnetic resonance study of the thioltransferase-catalyzed glutathione/glutathione disulfide interchange reaction
36. Evidence for a general relationship between the induced level of DNA double-strand breakage and cell-killing after X-irradiation of mammalian cells
37. Evidence for the presence of thioltransferase in the lens
38. Redox signaling and the emerging therapeutic potential of thiol antioxidants
39. Isolation and characterization of glucose-6-phosphate dehydrogenase deficient chinese hamster cells derived from pure mutant colonies
40. Glucose-6-phosphate dehydrogenase and the oxidative pentose phosphate cycle protect cells against apoptosis induced by low doses of ionizing radiation
41. Sensitivity to chemical oxidants and radiation in CHO cells deficient in oxidative pentose cycle activity
42. Repair of doublestrand DNA breaks correlates with radiosensitivity of L5178Y and L5178Y-R cells
43. Reduced sulfhydryl groups are required for DNA binding of Ku protein