The trans-activation domain of the sporulation response regulator Spo0A revealed by X-ray crystallography

Molecular Microbiology

Volumn 38, Issue 2, 2000, Pages 198-212

  Lewis, Richard J ^a   Krzywda, Szymon ^a   Brannigan, James A ^a   Turkenburg, Johan P ^a   Muchová, Katarína ^b   Dodson, Eleanor J ^a   Barák, Imrich ^a   Wilkinson, Anthony J ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b INSTITUTE OF CHEMISTRY   (Slovakia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PROTEIN SPOOA; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS; BACTERIAL GENE; CRYSTAL STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; SPOROGENESIS; TRANSACTIVATION; TRANSCRIPTION INITIATION; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACILLUS STEAROTHERMOPHILUS; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; HELIX-TURN-HELIX MOTIFS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SOLUTIONS; SPORES, BACTERIAL; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); EUKARYOTA; GEOBACILLUS STEAROTHERMOPHILUS; PROKARYOTA;

EID: 0033745991     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.02134.x     Document Type: Article

References (57)

1. Dimer form of phosphorylated Spo0A, a transcriptional regulator, stimulates the spo0F transcription at the initiation of sporulation in Bacillus subtilis
2. Structure of the Escherichia coli response regulator NarL
3. Phosphorylation of Bacillus subtilis transcription factor Spo0A stimulates transcription from the spollG promoter by enhancing binding to weak 0A boxes
4. Conformational changes induced by phosphorylation of the FixJ receiver domain
5. The Bacillus subtilis response regulator Spo0A stimulates transcription of the spollG operon through modification of RNA polymerase promoter complexes
6. Characterization of Spo0A homologs in diverse Bacillus and Clostridium species identifies a probable DNA-binding domain
7. A region in Bacillus subtilis σ(H) required for Spo0A-dependent promoter activity
8. A region n the Bacillus subtilis transcription factor Spo0A that is important for spollG promoter activation
9. Initiation of sporulation in Bacillus subtilis is controlled by a multi-component phosphorelay
10. Promoter structure, promoter recognition and transcription activation in prokaryotes
11. NMR structure of activated CheY
12. Gene expression in single cells of Bacillus subtilis: Evidence that a threshold mechanism controls the initiation of sporulation
13. 'DM': An automated procedure for phase improvement by density modification
14. Structural basis for methylesterase CheB regulation by a phosphorylation-activated domain
15. The transcriptional profile of early to middle sporulation in Bacillus subtilis
16. Characterisation of the spo0A locus and its deduced product
17. Solution structure of the LexA repressor DNA-binding domain determined by H-1-NMR spectroscopy
18. Mutant forms of Spo0A that affect sporulation initiation: A general model for phosphorylation mediated activation of bacterial signal transduction proteins
19. The Spo0A protein of Bacillus subtilis inhibits transcription of the abrB gene without preventing binding of the polymerase to the promoter
20. Subunit composition and domain structure of the Spo0A sporulation transcription factor of Bacillus subtilis
21. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse
22. Spo0A mutants of Bacillus subtilis with sigma factor-specific defects in transcription activation
23. Regulation of the phosphorelay and the initiation of sporulation in Bacillus subtilis
24. Protein structure comparison by alignment of distance matrices
25. Functional map of the α subunit of Escherichia coli RNA polymerase: Two modes of transcription activation by positive factors
26. Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor
27. Gene encoding σ(E) is transcribed from a σ(A)-like promoter in Bacillus subtilis
28. Escherichia coli positive regulator OmpR has a large loop structure at the putative RNA polymerase interaction site
29. 70 that results in defects on phage λ repressor stimulated transcription
30. Phosphorylated aspartate in the structure of a response regulator protein
31. Domain swapping in the sporulation response regulator Spo0A
32. 70 subunit
33. 70 subunit of RNA polymerase in transcriptional activation by activator protein PhoB in Escherichia coli
34. The DNA-binding domain of OmpR: Crystal structure of a winged helix transcription factor
35. Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A
36. Refinement of macromolecular structures by the maximum-likelihood method
37. Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box
38. Novel mutations that alter regulation of sporulation in Bacillus subtilis: Evidence that phosphorylation of regulatory protein Spo0A controls the initiation of sporulation
39. Processing of diffraction data collected in oscillation mode
40. Structure of the gene for the transition state regulator abrB: Regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis
41. Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis
42. Contributions of the domains of the Bacillus subtilis response regulator Spo0A to transcription stimulation of the spollG operon
43. Identification of a second region of the Spo0A response regulator of Bacillus subtilis required for transcription activation
44. Spo0A binds to a promoter used by σ(A) RNA polymerase during sporulation in Bacillus subtilis
45. A new mutation in Spo0A with intragenic suppressors in the effector domain
46. Activation of the Bacillus subtilis spollG promoter requires interaction of Spo0A and the sigma subunit of RNA polymerase
47. Structural alterations in the Bacillus subtilis Spo0A regulatory protein which suppresses mutations at several spo0 loci
48. Transcription regulation by the Spo0A protein of Bacillus subtilis
49. The Spo0A protein of Bacillus subtilis is a repressor of the abrB gene
50. Spo0A activates and represses its own synthesis by binding at its dual promoters
51. A positive feedback loop controls transcription of the spo0F gene, a component of the sporulation phosphorelay in Bacillus subtilis
52. Automated MAD and MIR structure solution
53. Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay
54. Regulation of spo0H, a gene coding for the Bacillus subtilis sigma-H factor
55. The locus of sequence-directed and protein-induced DNA bending
56. Transcription of the Bacillus subtilis spollA locus
57. Spo0A controls the σ(A) dependent activation of Bacillus subtilis sporulation specific transcription unit spollE