Spo0A mutants of Bacillus subtilis with sigma factor-specific defects in transcription activation

Journal of Bacteriology

Volumn 180, Issue 14, 1998, Pages 3584-3591

  Hatt, Janet K ^a   Youngman, Philip ^b  

^a University of Georgia ^*  (United States)

^b MILLENNIUM PHARMACEUTICALS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MUTANT PROTEIN; RNA POLYMERASE; SIGMA FACTOR; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR SPO0A; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL SPORE; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; SPOROGENESIS; TRANSCRIPTION INITIATION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0032447903     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.14.3584-3591.1998     Document Type: Article

References (43)

1. Albright, L. M., E. Huala, and F. M. Ausubel. 1989. Prokaryotic signal transduction mediated by sensor and regulator protein pairs. Annu. Rev. Genet. 23:311-336.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. A. Struhl. 1994. Current protocols in molecular biology. John Wiley and Sons, Inc., New York, N.Y.
3. Backman, K., M. Ptashne, and W. Gilbert. 1976. Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc. Natl. Acad. Sci. USA 73:4174-4178.
4. Baldus, J. M., C. M. Buckner, and C. P. Moran, Jr. 1995. Evidence that the transcriptional activator Spo0A interacts with two sigma factors in Bacillus subtilis. Mol. Microbiol. 17:281-290.
5. Baldus, J. M., B. D. Green, P. Youngman, and C. P. Moran, Jr. 1994. Phosphorylation of Bacillus subtilis transcription factor Spo0A stimulates transcription from the spoIIG promoter by enhancing binding to weak 0A boxes. J. Bacteriol. 176:296-306.
6. Bird, T. H., J. K. Grimsley, J. A. Hoch, and G. B. Spiegelman. 1996. The Bacillus subtilis response regulator Spo0A stimulates transcription of the spoIIG operon through modification of RNA polymerase promoter complexes. J. Mol. Biol. 256:436-448.
7. Brown, D. P., L. Ganova-Raeva, B. D. Green, S. R. Wilkinson, M. Young, and P. Youngman. 1994. Characterization of spo0A homologues in diverse Bacillus and Clostridium species identifies a probable DNA-binding domain. Mol. Microbiol. 14:411-426.
8. Buckner, C. M., G. Schyns, and C. P. Moran, Jr. 1998. A region in the Bacillus subtilis transcription factor Spo0A that is important for spoIIG promoter activation. J. Bacteriol. 180:3578-3583.
9. Burbulys, D., K. A. Trach, and J. A. Hoch. 1991. Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell. 64:545-552.
10. de Crombrugghe, B., S. Busby, and H. Buc. 1984. Cyclic AMP receptor protein: role in transcription activation. Science 224:831-838.
11. H). J. Bacteriol. 170:1054-1062.
12. Guzmán, P., J. Westpheling, and P. Youngman. 1988. Characterization of the promoter region of the Bacillus subtilis spoIIE operon. J. Bacteriol. 170:1598-1609.
13. Harwood, C. R., and S. M. Cutting (ed.). 1990. Molecular biological methods for Bacillus. John Wiley and Sons Ltd., Chichester, United Kingdom.
14. Hoch, J. A. 1993. The phosphorelay signal transduction pathway in the initiation of Bacillus subtilis sporulation. J. Cell. Biochem. 51:55-61.
15. Igarashi, K., A. Hanamura, K. Makino, H. Aiba, H. Aiba, T. Mizuno, A. Nakata, and A. Ishihama. 1991. Functional map of the alpha subunit of Escherichia coli RNA polymerase: two modes of transcription activation by positive factors. Proc. Natl. Acad. Sci. USA 88:8958-8962.
16. Ireton, K., and A. D. Grossman. 1992. Coupling between gene-expression and DNA-synthesis early during development in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 89:8808-8812.
17. Ireton, K., D. Z. Rudner, K. J. Siranosian, and A. D. Grossman. 1993. Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor. Genes Dev. 7:283-294.
18. Ishihama, A. 1993. Protein-protein communication within the transcription apparatus. J. Bacteriol. 175:2483-2489.
19. Karow, M. L., and P. J. Piggot. 1995. Construction of gusA transcriptional fusion vectors for Bacillus subtilis and their utilization for studies of spore formation. Gene 163:69-74.
20. Kenney, T. J., and C. P. Moran, Jr. 1987. Organization and regulation of an operon that encodes a sporulation-essential sigma factor in Bacillus subtilis. J. Bacteriol. 169:3329-3339.
21. Kumar, A., B. Grimes, N. Fujita, K. Makino, R. A. Malloch, R. S. Hayward, and A. Ishihama. 1994. Role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation. J. Mol. Biol. 235:405-413.
22. LeBlanc, D. J., L. N. Lee, and J. M. Inamine. 1991. Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis. Antimicrob. Agents Chemother. 35: 1804-1810.
23. Leung, D. W., E. Chen, and D. V. Goeddel. 1989. A method for random mutagenesis of a defined DNA segment using modified polymerase chain reaction. Technique 1:11-15.
24. Levin, P., and R. Losick. Personal communication.
25. Li, M., H. Moyle, and M. M. Susskind. 1994. Target of the transcriptional activation function of phage lambda cI protein. Science 263:75-77.
26. Nicholson, W. L., and P. Setlow. 1990. Sporulation, germination and outgrowth, p. 391-450. In C. R. Harwood and S. M. Cutting (ed.). Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, United Kingdom.
27. Olmedo, G., E. G. Ninfa, J. Stock, and P. Youngman. 1990. Novel mutations that alter the regulation of sporulation in Bacillus subtilis - evidence that phosphorylation of regulatory protein Spo0A controls the initiation of sporulation. J. Mol. Biol. 215:359-372.
28. Perego, M., J. J. Wu, G. B. Spiegelman, and J. A. Hoch. 1991. Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis. Gene 100:207-212.
29. Perez-Casal, J., J. A. Price, E. Maguin, and J. R. Scott. 1993. An M protein with a single C repeat prevents phagocytosis of Streptococcus pyogenes: use of a temperature-sensitive shuttle vector to deliver homologous sequences to the chromosome of S. pyogenes. Mol. Microbiol. 8:809-819.
30. Piggot, P. J., and C. A. Curtis. 1987. Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions. J. Bacteriol. 169:1260-1266.
31. A RNA polymerase during sporulation in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 88:4533-4537.
32. Satola, S. W., J. M. Baldus, and C. P. Moran, Jr. 1992. Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis. J. Bacteriol. 174: 1448-1453.
33. Schyns, G., C. M. Buckner, and C. P. Moran, Jr. 1997. Activation of the Bacillus subtilis spoIIG promoter requires interaction of Spo0A and the sigma subunit of RNA polymerase. J. Bacteriol. 179:5605-5608.
34. Stock, J. B., A. J. Ninfa, and A. M. Stock. 1989. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol. Rev. 53:450-490.
35. Strauch, M., V. Webb, G. Spiegelman, and J. A. Hoch. 1990. The Spo0A protein of Bacillus subtilis is a repressor of the abrB gene. Proc. Natl. Acad. Sci. USA 87:1801-1805.
36. Strauch, M. A., K. A. Trach, J. Day, and J. A. Hoch. 1992. Spo0A activates and represses its own synthesis by binding at its dual promoters. Biochimie 74:619-626.
37. Trach, K., D. Burbulys, M. Strauch, J. J. Wu, N. Dhillon, R. Jonas, C. Hanstein, P. Kallio, M. Perego, T. Bird, G. Spiegelman, C. Fogher, and J. A. Hoch. 1991. Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay. Res. Microbiol. 142:815-823.
38. Wu, J. J., M. G. Howard, and P. J. Piggot. 1989. Regulation of transcription of the Bacillus subtilis spoIIA locus. J. Bacteriol. 171:692-698.
39. Wu, J. J., P. J. Piggot, K. M. Tatti, and C. P. Moran, Jr. 1991. Transcription of the Bacillus subtilis spoIIA locus. Gene 101:113-116.
40. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
41. A-dependent activation of Bacillus subtilis sporulation-specific transcription unit spoIIE. J. Bacteriol. 174:2548-2658.
42. Youngman, P. 1987. Plasmid vectors for recovering and exploiting Tn9/7 transpositions in Bacillus and other gram-positive bacteria, p. 79-103. In K. Hardy (ed.), Plasmids: a practical approach. IRL Press, Oxford, United Kingdom.
43. Youngman, P., J. B. Perkins, and R. Losick. 1984. Construction of a cloning site near one end of Tn9/7 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene. Plasmid 12:1-9.