Purification and recognition of recombinant mouse P2X 1 receptors expressed in a baculovirus system

Drug Development Research

Volumn 51, Issue 1, 2000, Pages 7-19

  Chen, Liping ^a   Hardwick, James P ^b   McPhie, Peter ^a   Sitkovsky, Michail V ^c   Jacobson, Kenneth A ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NORTHEAST OHIO MEDICAL UNIVERSITY   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Affinity chromatography; Agonist; Binding site; Ion channels; Nucleotides

Indexed keywords

6 N METHYL 2' DEOXYADENOSINE; 6 N METHYLADENOSINE; ADENOSINE TRIPHOSPHATE DERIVATIVE; ALPHA,BETA METHYLENEADENOSINE TRIPHOSPHATE; DEOXYADENOSINE DERIVATIVE; PURINE P2X RECEPTOR; PYRIDOXAL PHOSPHATE 6 AZOPHENYL 2',4' DISULFONIC ACID; RECEPTOR SUBUNIT; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; AUTORADIOGRAPHY; BACULOVIRUS; CIRCULAR DICHROISM; DRUG RECEPTOR BINDING; EXPRESSION VECTOR; ISOTOPE LABELING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; RECEPTOR SOLUBILIZATION;

EID: 0033733764     PISSN: 02724391     EISSN: None     Source Type: Journal    
DOI: 10.1002/1098-2299(20000901)51:1<7::AID-DDR2>3.0.CO;2-W     Document Type: Article

References (49)

1. Cleavage at the Asn-Gly bonds with hydroxylamine
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
3. 1 receptor activation in HL60 cells
4. cDNA-directed expression of human cytochrome P450 CYP1A1 using baculovirus
5. Coexpression of cytochrome P4502A6 and human NADPH-P450 oxidoreductase in the baculovirus system
6. Affinity comparison of ligands binding to recombinant human A(2A) adenosine receptors using surface plasmon resonance
7. P2X receptors in autonomic and sensory neurons
8. Ionic permeability of, and divalent cation effects on, two ATP-gated cation channels (P2X receptors) expressed in mammalian cells
9. Further comparison of contractions of the smooth muscle of the guinea-pig isolated vas deferens induced by ATP and related analogs
10. Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin
11. A new inhibitor of the chymotrypsin-like activity of the multicatalytic proteinase complex (20S proteasome) induces accumulation of ubiquitin-protein conjugates in a neuronal cell
12. Characterization of myocardial extracellular ATP receptors by photoaffinity labelling and functional assays
13. 2(X) receptors
14. Structure activity relationships of P2 receptor agonists and antagonists
15. A pyridoxine cyclic-phosphate and its 6-arylazo-derivative selectively potentiate and antagonize activation of P2X1 receptors
16. Enhancement of cyanogen bromide cleavage yields for methionyl-serine and methionyl-threonine peptide bonds
17. 2, an ATP-gated ion channel
18. 6 channel subunits coassemble into a novel heterometric ATP receptor
19. Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels
20. Mg2+ inhibition of ATP-activated current in rat nodose ganglion neurons: Evidence that Mg2+ decreases the agonist affinity of the receptor
21. Conformational maturation of CFTR but not its mutant counterpart (ΔF508) occurs in the endoplasmic reticulum and requires ATP
22. 2(T) purinoceptors in human platelets
23. Degradation of 3-hydroxy-3-methylglutaryl-CoA reductase in endoplasmic reticulum membranes is accelerated as a result of increased susceptibility to proteolysis
24. Structure of the hormone binding domain of human beta 1 thyroid hormone nuclear receptor: Is it an alpha/beta barrel?
25. 35S]-adenosine 5'-O-[3-thiotriphosphate] in rat vas deferens membranes
26. 4 purinoceptor: Evidence for allosteric interactions of purinoceptor antagonists and monovalent cations with p2X purinoceptors
27. Degradation of HMG-CoA reductase in vitro
28. 3 receptors form stable trimers: A novel structural motif of ligand-gated ion channels
29. P2X receptors: A third major class of ligand-gated ion channels
30. Pharmacology of cloned P2X receptors
31. 1 receptor) activation and desensitization by the actin cytoskeleton
32. Phosphorylated human keratinocyte ornithine decarboxylase is preferentially associated with insoluble cellular proteins
33. Estimation of globular protein secondary structure from circular dichroism
34. 3 receptors
35. Receptors for purines and pyrimidines
36. 2(X)-purinoceptor in cultured neurons of the rat dorsal root ganglia
37. Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules
38. Tricine sodium dodecy sulfate polyacrylamide gel elecrophoresis for the separation of proteins in the range of 1 to 100kDA
39. P2X4: An ATP-activated ionotropic receptor cloned from rat brain
40. Cloned ligand-gated channels activated by extracellular ATP (P2X receptors)
41. The drosophila lethal(2)giant larvae tumor suppressor protein is a component of the cytoskeleton
42. 2(X) receptors bring new structure to ligand-gated ion channels
43. Two-dimensional peptide mapping by polyacrylamide-gel electrophoresis with limited proteolysis in SDS
44. 5 receptor subunits reveals a novel ATP-gated ion channel
45. N-Linked glycosylation is essential for the functional expression of the recombinant P2X2 receptor
46. A new class of ligand-gated ion channel defined by P2X receptor for extraeelhdar ATP
47. Characterization and chromosomal localization of a human P2X receptor from the urinary bladder
48. Domains of P2X receptors involved in desensitization