Detection of virtually all mutations-SSCP (DOVAM-S): A rapid method for mutation scanning with virtually 100% sensitivity

BioTechniques

Volumn 26, Issue 5, 1999, Pages 932-942

  Liu, Q ^a   Feng, J ^a   Buzin, C ^a   Wen, C ^a   Nozari, G ^a   Mengos, A ^a   Nguyen, V ^a   Liu, J ^a   Crawford, L ^a   Fujimura, E K ^a   Sommer, S S ^a  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 9; DNA;

ARTICLE; DIAGNOSTIC ACCURACY; DNA FINGERPRINTING; DNA SEQUENCE; ELECTROPHORESIS; GENE MUTATION; GENE SEQUENCE; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN SECONDARY STRUCTURE; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0032915812     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99265rr03     Document Type: Article

References (13)

1. Glavac, D. and M. Dean. 1993. Optimization of the single-strand conformation polymorphism (SSCP) technique for detection of point mutations. Hum. Mutat. 2:404-414.
2. Hayashi, K. and D.W. Yandell. 1993. How sensitive is PCR-SSCP? Hum. Mutat. 2:338-346.
3. Hongo, T., G.S. Buzard, RJ. Calvert and CM. Weghorst. 1993. 'Cold SSCP': a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses. Nucleic Acids Res. 27:3637-3642.
4. Liu, Q., J. Feng and S.S. Sommer. 1996. Bidirectional dideoxy fingerprinting (Bi-ddF): a rapid method for quantitative detection of regions of 300-600 bp. Hum. Mol. Genet. 5:107-114.
5. Liu, Q., J. Feng and S.S. Sommer. 1997. In a blinded analysis, restriction endonuclease fingerprinting detects all the mutations in a 1.9-kb segment. BioTechniques 25:836-839.
6. Liu, Q. and S.S. Sommer. 1994. Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP. PCR Methods Appl. 4:97-108.
7. Liu, Q. and S.S. Sommer. 1998. The SSCP phenomenon: addition of HEPES buffer dramatically affects electrophoretic mobility. BioTechniques 25:50-56.
8. Michaud, J., L.C. Brody, G. Steel, G. Fontaine, L.S. Martin, D. Valle and G. Mitchell. 1992. Strand-separating conformational polymorphism analysis: efficacy of detection of point mutations in the human ornithine δ-aminotransferase gene. Genomics 73:389-394.
9. Sarkar, G., H. Yoon and S.S. Sommer. 1992. Dideoxy fingerprinting (ddF): a rapid and efficient screen for the presence of mutations. Genomics 73:441-443.
10. Sarkar, G., H. Yoon and S.S. Sommer. 1992. Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP. Nucleic Acids Res. 20:871-878.
11. Sheffield, V.C., J.S. Beck, A.E. Kwitek, D.W. Sandstrom and E.M. Stone. 1993. The sensitivity of single-strand conformation polymorphism analysis for the detection of single base substitutions. Genomics 76:325-332.
12. Takahashi-Fujii, A., Y. Ishino, A. Shimada and I. Kalo. 1993. Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations. PCR Methods Appl. 2:323-327.
13. Yoshitake, S., B.C. Schach, D.C. Foster, E.W. Davie and K. Kurachi. 1985. Nucleotide sequence of the gene for human factor IX (antihemophilic factor B). Biochemistry 24:3736-3750.