Non-biotin amplification (NBA) kit prevents nonspecific background staining of endogenous biotin induced by heat induced epitope retrieval (HIER) procedure

Journal of Histotechnology

Volumn 23, Issue 4, 2000, Pages 327-331

  Shi, Z R ^a   Au, A ^a   Soriano, R ^a   Zhao, J X ^a   Sweet, W L ^a  

^a SAN FRANCISCO STATE UNIVERSITY   (United States)

Author keywords

Biotin labeled; Endogenous avidin binding activity; Routinely processed tissue; Streptavidin

Indexed keywords

BIOTIN; STREPTAVIDIN;

ANTIGEN RETRIEVAL; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; HUMAN; HUMAN TISSUE; REHYDRATION; TISSUE FIXATION;

EID: 0033638979     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.2000.23.4.327     Document Type: Article

References (9)

1. Standarization and further development of antigen retrieval immunohistochemistry: Strategies and future goals
2. Antigen retrieval in formalin-fixed paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
3. Endogenous avidin-binding activity in paraffin-embedded tissue revealed after microwave treatment
4. Blocking of endogenous avidin-binding activity in immunohistochemistry
5. Retrieved endogenous biotin: A noval marker and a potential pitfall in diagnostic immunohistochemistry
6. Cytoplasmic biotin-like activity interferes with immunohistochemical analysis of thyroid lesions: A comparison of antigen retrieval methods
7. Quality assurance issues in immunohistochemistry
8. A comparison of three immunoperoxidase techniques for antigen detection in colorectal carcinoma tissues
9. Sensitivity and detection efficiency of the peroxidase antiperoxidase (PAP), avidin-biotin peroxidase complex (ABC), and peroxidase-labeled avidin-biotin (LAB) methods