Endogenous avidin-binding activity in paraffin-embedded tissue revealed after microwave treatment

Applied Immunohistochemistry and Molecular Morphology

Volumn 5, Issue 1, 1997, Pages 59-62

  Rodriguez Soto, Jorge ^a,b   Warnke, Roger A ^a   Rouse, Robert V ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b PALO ALTO MEDICAL FOUNDATION   (United States)

Author keywords

Antibody staining; Antigen retrieval; Avidin; Biotin

Indexed keywords

AVIDIN; BIOTIN;

ARTICLE; CONTROLLED STUDY; EPITOPE RETRIEVAL; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; KIDNEY; LIVER; MICROWAVE RADIATION; PRIORITY JOURNAL; TECHNIQUE;

EID: 0030912386     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199703000-00009     Document Type: Article

References (6)

1. Gown AM, de Wever N, Battifora H. Microwaved-based antigenic unmasking: a revolutionary new technique for routine immunohistochemistry. Appl Immunohistochem 1993;1:256-66.
2. Miller K, Auld J, Jessup E, Rhodes A, Ashton-Key M. Antigen unmasking in formalin-fixed routinely processed paraffin wax-embedded sections by pressure cooking: a comparison with microwave oven heating and traditional methods. Adv Anat Pathol 1995; 2:2-9.
3. Dakshinamurti K, Mistry SP. Tissue and intracellular distribution of biotin-C14OOH in rats and chicks. J Biol Chem 1963;238: 294-6.
4. Moss J, Lane MD. The biotin-dependent enzymes. Adv Enzymol 1971;35:321-3.
5. Wood HG, Borden R. Biotin Enzymes. Annu Rev Biochem 1977; 46:385-413.
6. Wood GS, Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981;29:1196-204.