Blocking of endogenous avidin-binding activity in immunohistochemistry: The use of egg whites

Applied Immunohistochemistry and Molecular Morphology

Volumn 5, Issue 1, 1997, Pages 63-66

  Miller, Rodney T ^a   Kubier, Patty ^a  

^a ProPath Laboratories Inc   (United States)

Author keywords

Avidin; Biotin; Eggs; Immunocytochemistry; Immunohistochemistry; Methods

Indexed keywords

AVIDIN; EGG WHITE;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; TECHNIQUE;

EID: 0031005805     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199703000-00010     Document Type: Article

References (6)

1. Wood GS, Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981;29(10):1196-204.
2. Miller RT, Estran CE. Heat-induced epitope retrieval with a pressure cooker: Suggestions for optimal use. Appl Immunohistochem 1995;3(3):190-3.
3. Miller RT. Multitumor "sandwich" blocks in immunohistochemistry. Simplified method of preparation and practical uses. Appl Immunohistochem 1993;1:156-9.
4. Taylor, CR, Shi SR, Chaiwun B, et al. Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by the antigen retrieval technique. Hum Pathol 1994;25:263-70.
5. Beckstead JH. Improved antigen retrieval in formalin-fixed paraffin embedded tissue. Appl Immunohistochem 1994;2:274-81.
6. Butz WR, Clark KC, Miller RT. Improved manual immunohistochemistry employing orbital mixing of reagents during incubations. Appl Immunohistochem 1994;2:65-7, 1994.