Characterization and modulation of [125I]iberiotoxin-D19Y/Y36F binding in the guinea-pig urinary bladder

European Journal of Pharmacology

Volumn 388, Issue 2, 2000, Pages 155-161

  Molinari, Eduardo J ^a   Sullivan, James P ^a   Wan, Yieh Ping ^a   Brioni, Jorge D ^a   Gopalakrishnan, Murali ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

Iberiotoxin; Maxi K+ channel; Radioligand binding; Urinary bladder

Indexed keywords

CALCIUM ACTIVATED POTASSIUM CHANNEL; IBERIOTOXIN D19Y Y36F; IBERIOTOXIN-D19Y-Y36F; LARGE CONDUCTANCE CALCIUM ACTIVATED POTASSIUM CHANNEL; LIGAND; PEPTIDE; POTASSIUM CHANNEL; APAMIN; EDETIC ACID; EGTAZIC ACID; IBERIOTOXIN; INDOLE ALKALOID; IODINE 125; MARGATOXIN; N (4 BENZOYLPHENYL) 3,3,3 TRIFLUORO 2 HYDROXY 2 METHYLPROPIONAMIDE; PAXILLINE; RADIOLIGAND;

ANIMAL; ARTICLE; BLADDER; DRUG EFFECT; GUINEA PIG; IN VITRO STUDY; KINETICS; MALE; MEMBRANE; METABOLISM; RADIOASSAY; ANIMAL TISSUE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; GUINEA PIGS; KINETICS; LARGE-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNELS; LIGANDS; MALE; MEMBRANES; PEPTIDES; POTASSIUM CHANNELS; POTASSIUM CHANNELS, CALCIUM-ACTIVATED; RADIOLIGAND ASSAY; URINARY BLADDER;

EID: 0033621963     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(99)00853-5     Document Type: Article

References (27)

1. Brading A.F., Teramoto N., Nakayama S., Bramich N., Inoue R., Fujii K., Mostwin K. The relationship between the electrophysiological properties of lower urinary tract smooth muscles and their function in vivo. Bolton T.B., Tomita T. Smooth Muscle Excitation. 1996;403-415 Academic Press, San Diego.
2. 50) of an enzymatic reaction. Biochem. Pharmacol. 22:1973;3099-3108.
3. + channel blockers paspalitrem-C and paxilline on mammalian smooth muscle. Eur. J. Pharmacol. 314:1996;123-128.
4. Deutsch C., Price M., Lee S., King V.F., Garcia M.L. Characterization of high affinity binding sites for charybdotoxin in human T lymphocytes. J. Biol. Chem. 266:1991;3668-3674.
5. KCa channel opener, in vascular smooth muscle. Br. J. Pharmacol. 113:1994;1538-1547.
6. Garcia-Calvo M., Knaus H.G., McManus O.B., Giangiacomo K.M., Kaczorowski G.J., Garcia M.L. Purification and reconstitution of the high-conductance, calcium-activated potassium channel from tracheal smooth muscle. J. Biol. Chem. 269:1994;676-682.
7. + channels by pinacidil, cromakalim, and glibenclamide. Am. J. Physiol. 264:1993;C1119-C1127. (Cell Physiol.).
8. Giangiacomo K.M., Garcia-Calvo M., Hans-Gunther K., Mullmann T.J., Garcia M.L., McManus O. Functional reconstitution of the large-conductance, calcium-activated potassium channel purified from bovine aortic smooth muscle. Biochemistry. 34:1995;15849-15862.
9. Gribkoff V.K., Lum-Ragan J.T., Boissard C.G., Post-Minson D.J., Meanwell N.A., Starret J.E., Kozlowski E.S., Romine J.L., Trojnacki J.T., McKay C., Zhong J., Dworetzky S.I. Effects of channel modulators on cloned large-conductance calcium-activated potassium channels. Mol. Pharmacol. 50:1996;206-217.
10. Hanner M., Schmalhofer W.A., Munujos P., Knaus H.-G., Kaczorowski G.J., Garcia M.L. The β subunit of the high-conductance calcium-activated potassium channel contributes to the high-affinity receptor for charybdotoxin. Proc. Natl. Acad. Sci. U.S.A. 94:1997;2853-2858.
11. + channels regulate action potential repolarization in urinary bladder smooth muscle. Am. J. Physiol. 273:1997;C110-C117.
12. ATP channel opener with in vivo selectivity for urinary bladder. J. Pharmacol. Exp. Ther. 274:1995;884-890.
13. + channels by Zeneca ZD6169 in guinea pig bladder smooth muscle cells. J. Pharmacol. Exp. Ther. 280:1997;38-45.
14. 2+ storage site in some types of smooth muscle cells. Bolton T.B., Tomita T. Smooth Muscle Excitation. 1996;337-354 Academic Press, San Diego.
15. Kaczorowski G.J., Knaus H.-G., Leonard R.J., McManus O.B., Garcia M.L. High conductance calcium-activated potassium channels: structure, pharmacology and function. J. Bioenerg. Biomemb. 28:1996;255-267.
16. Klöckner U., Isenberg G. Action potential and net membrane currents of isolated smooth muscle cells (urinary bladder of the guinea pig). Pflügers Arch. 405:1985;329-339.
17. + channel. J. Biol. Chem. 270:1995;22434-22439.
18. Knaus H.G., McManus O.B., Lee S.H., Schmalhofer A., Garcia-Calvo M., Helms L.M.H., Sanchez M., Giangiacomo K., Reuben J.P., Smith A.B. III, Kaczorowski G.J., Garcia M.L. Tremorgenic indole alkaloids potently inhibit smooth muscle high-conductance calcium-activated potassium channels. Biochemistry. 33:1994;5819-5828.
19. 125]iberiotoxin-D19Y/Y36F, the first selective, high specific activity radioligand for high-conductance calcium-activated potassium channels. Biochemistry. 36:1997;1943-1952.
20. Latorre R., Oberhauser A., Labarca P., Alvarez O. Varieties of calcium-activated potassium channels. Annu. Rev. Physiol. 51:1989;385-399.
21. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
22. Marty A. Ca-dependent K channels with large unitary conductance in chromaffin cell membranes. Nature. 291:1981;497-500.
23. Suarez-Kurtz G., Garcia M.L., Kaczorowski G.J. Effect of charybdotoxin on spontaneous motility and tonus of different guinea-pig smooth muscle tissues. J. Pharmacol. Exp. Ther. 259:1991;439-443.
24. Taylor J.W., Bidard J.-N., Lazdunski M. The characterization of high affinity binding sites in rat brain for the mast cell degranulating peptide from bee venom using the purified monoiodinated peptide. J. Biol. Chem. 259:1984;13957-13967.
25. 3H-P1075 binding in bladder smooth muscle. Pharmacology. 50:1995;388-397.
26. Vàzquez G., Feigenbaum P., King V.F., Kaczorowki G.J., Garcia M.L. Characterization of high affinity binding sites for charybdotoxin in synaptic plasma membranes from rat brain. J. Biol. Chem. 265:1990;15564-15571.
27. Vàzquez J., Feigenbaum P., Katyz G., King V.F., Reuben J.P., Roy-Contancin L., Slaughter R.S., Kaczorowski G.J., Garcia M.L. Characterization of high affinity binding sites for charybdotoxin in sarcolemmal membranes from bovine aortic smooth muscle. J. Biol. Chem. 264:1989;20902-20909.