Generation of self-antigen reactive, anti-urocortin specific antibodies by immunization of recombinantly expressed urocortin fusion proteins

Molecules and Cells

Volumn 9, Issue 6, 1999, Pages 587-595

  Na, Shin Young ^a   Park, Jung Hyun ^a   Cho, Eun Wie ^a   You, Kwan Hee ^b   Kim, Kil Lyong ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

6X Histidine; Antibodies; Glutathione S transferase; Maltose Binding Protein; Thioredoxin; Urocortin

Indexed keywords

ABC TRANSPORTER; CARRIER PROTEIN; CORTICOTROPIN RELEASING FACTOR; ESCHERICHIA COLI PROTEIN; GLUCOSE TRANSPORTER; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MALTOSE TRANSPORT SYSTEM, E COLI; MALTOSE-BINDING PROTEIN; THIOREDOXIN; UROCORTIN;

ANIMAL; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETICS; IMMUNIZATION; IMMUNOLOGY; MOLECULAR CLONING; MOUSE; RAT; WESTERN BLOTTING;

ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BLOTTING, WESTERN; CARRIER PROTEINS; CLONING, MOLECULAR; CORTICOTROPIN-RELEASING HORMONE; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLUTATHIONE TRANSFERASE; IMMUNIZATION; MICE; MONOSACCHARIDE TRANSPORT PROTEINS; RATS; RECOMBINANT FUSION PROTEINS; THIOREDOXIN;

EID: 0033621305     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Bagavant, H., Fusin, F. M., Baisch, J., Kurth, B., David, C. S., and Tung, K. S. K. (1997) Immunogenicity and contraceptive potential of a zona pellucida 3 peptide vaccine. Biol. Reprod. 56, 764-770.
2. Bamberger, C. M., Wald, M., Bamberger, A. M., Erguen, S., Beil, F. U., and Schulte, H. M. (1998) Human lymphocytes produce urocortin, but not corticotropin-releasing hormone. J. Clin. Endocrinol. Metab. 83, 708-711.
3. Beers, E. P. and Callis, J. (1993) Utility of polyhistidine-tagged ubiquitin in the purification of ubiquitin-protein conjugates and as an affinity ligand for the purification of ubiquitin-specific hydrolases. J. Biol. Chem. 268, 21645-21649.
4. 1 receptor mRNA expression. J. Neurosci. 15, 6341-6350.
5. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
6. Chopra, A. K., Brasier, A. R., Das, M., Xu, X. J., and Peterson, J. W. (1994) Improved synthesis of Salmonella typhimurium enterotoxin using gene fusion expression systems. Gene 144, 81-85.
7. Dalum, I., Jensen, M. R., Hindersson, P., Elsner, H. I., and Mouritsen, S. (1996) Breaking of B-cell tolerance toward a highly conserved self protein. J. Immunol. 157, 4796-4804.
8. Dalum, I., Jensen, M. R., Gregorius, K., Thomasen, C. M., Elsner, H. I., and Mouritsen, S. (1997) Induction of cross-reactive antibodies against a self protein by immurization with a modified self protein containing a foreign T-helper epitope. Mol. Immunol. 34, 1113-1120.
9. Derman, A. I., Prinz, W. A., Belin, D., and Beckwith, J. (1993) Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science 262, 1744-1747.
10. Frangioni, J. V. and Neel, B. G. (1993) Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal. Biochem. 210, 170-187.
11. Hara, Y., Ueta, Y., Isse, T., Kabashima, N., Shibuya, I., Hattori, Y, and Yamashitu, H. (1997) Increase of urocortin-like immunoreactivity in the rat hypothalamo-neurohypophysial system after salt loading and hypophysectomy. Neurosci. Letters 227, 127-130.
12. Ikeda, K., Tojo, K., Sato, S., Ebisawa, T., Tokudome, G., Hosoya, T., Harada, M., Nakagawa, O., and Nakao, K. (1998) Urocortin, a newly identified corticotropin-releasing factor-related mammalian peptide, stimulates atrial natriuretic peptide and brain natriuretic peptide secretions from neonatal rat cardiomyocytes. Biochem. Biophys. Res. Commun. 250, 298-304.
13. LaVallie, E. R., DiBlasio, E. A., Kovacic, S., Grant, K. L., Schendel, P. F., and McCoy, J. M. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 11, 187-193.
14. 2β receptor mRNAs are differentially distributed between the rat central nervous system and peripheral tissues. Endocrinology 136, 4139-4142.
15. Maina, C. V., Riggs, P. D., Grandea, A. G., Slatko, B. E., Moran, L. S., Tagliamonte, J. A., McReynolds, L. A., and di Guan, C. (1988) An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74, 365-373.
16. Mambetisaeva, E. T., Martin, P. E. M., and Evans, W. H. (1997) Expression of three functional domains of connexin 32 as thioredoxin fusion proteins in Escherichia coli and generation of antibodies. Prot. Expr. Purif. 11, 26-34.
17. Mamula, M. J., Lin, R. H., Janeway, C. A. Jr., and Hardin, J. A. (1992) Breaking T-cell tolerance with foreign and self co-immunogens. A study of autoimmune B- and T-cell epitopes of cytochrome c. J. Immunol. 149, 789-795.
18. Moreau, J.-L., Kilpatrick, G., and Jenck, F. (1997) Urocortin, a novel neuropeptide with anxiogenic-like properties. NeuroReport 8, 1697-1701.
19. Okamoto, M., Mitsuhara, I., Ohshima, M., Natori, S., and Ohashi, Y. (1998) Enhanced expression of an antimicrobial peptide sarcotoxin IA by GUS fusion in transgenic tobacco plants. Plant Cell Physiol. 39, 57-63.
20. Oki, Y., Iwabuchi, M., Masuzawa, M., Watanabe, F., Ozawa, M., lino, K., Tominaga, T., and Yoshimi, T. (1998) Distribution and concentration of urocortin, and effect of adrenalectomy on its content in rat hypothalamus. Life Sci. 62, 807-812.
21. Park, J. H., Cho, E. W., Lee, Y J., Hahm, K. S., and Kim, K. L. (1997) Generation and characterization of a novel fusion partner cell line for the production of human macrophage hybridoma. Hybridoma 16, 551-556.
22. x0405 molecules. J. Biochem. Mol. Biol. 31, 111-116.
23. Park, J. H., Choi, E. A., Cho, E. W., Hahm, K. S., and Kim, K. L. (1998a) Maltose binding protein (MBP) fusion proteins with low or no affinity to amylose resins can be single-step purified using a novel anti-MBP monoclonal antibody. Mol. Cells 8, 709-716.
24. Partidos, C. D., Obeid, O. E., and Steward, M. W. (1992) Antibody responses to non-immunogenic synthetic peptides induced by co-immunization with immunogenic peptides. Immunology 77, 262-266.
25. Perrin, M. H., Sutton, S., Bain, D. L., Berggren, W. T., and Vale, W. W. (1998) The first extracellular domain of corticotropin releasing factor-R1 contains major binding determinants for urocortin and astressin. Endocrinology 139, 566-570.
26. Petraglia, F., Florio, P., Gallo, R., Simoncini, T., Savozzi, M., Di Blasio, A. M., Vaughan, J., and Vale, W. (1996) Human placenta and fetal membrane express human urocortin mRNA and peptide. J. Clin. Endocrinol. Metab. 81, 3807-3810.
27. Sachdev, D. and Chirgwin, J. M. (1998) Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin. Prot. Exp. Purif. 12, 122-132.
28. Smith, D. B. and Johnson, K. S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
29. Spina, M., Merlo-Pich, E., Chan, R. K. W., Basso, A. M., Rivier, J., Vale, W., and Koob, G. F. (1996) Appetite-suppressing effects of urocortin, a CRF-related neuropeptide. Science 273, 1561-1564.
30. Turnbull, A. V. and Rivier, C. (1997) Corticotropin-releasing factor (CRF) and endocrine responses to stress: CRF receptors, binding protein, and related peptides. Proc. Soc. Exp. Biol. Med. 215, 1-10.
31. Vaughan, J., Donaldson, C., Bittencourt, J., Perrin, M. H., Lewis, K., Sutton, S., Chan, R., Turnbull, A. V., Lovejoy, D., Rivier, C., Rivier, J., Sawchenko, P. E., and Vale, W. (1995) Urocortin, a mammalian neuropeptide related to fish urotensin I and to corticotropin-releasing factor. Nature 378, 287-292.
32. Zhang, Y., Olsen, D. R., Nguyen, K. B., Olson, P. S., Rhodes, E. T., and Mascarenhas, D. (1998) Expression of eukaryotic proteins in soluble form in Eschericliia coli. Prot. Expr. Purif. 12, 159-165.