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Volumn 276, Issue 5321, 1997, Pages 2057-2062

Differential requirements for survival and proliferation of CD8 naive or memory T cells

Author keywords

[No Author keywords available]

Indexed keywords

T LYMPHOCYTE RECEPTOR;

EID: 0030873556     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5321.2057     Document Type: Article
Times cited : (742)

References (47)
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    • note
    • Host mice were irradiated at 600 rayleigh to spare BM precursors, but few peripheral cells were left. Peripheral T cell reconstitution requires previous recolonization of the thymus by host BM precursors, and host T cells start to emerge 1 month after irradiation. Irradiation may prevent the rejection of donor cells. It is also fundamental to allow quantification of T cell decays after adoptive transfer, because donor cells must be initially present at relatively high frequencies, requiring previous depletion of the host's lymphoid organs. In contrast to T cell decays, T cell survival or expansion can be quantified after adoptive transfer into intact mice.
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    • note
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    • note
    • Bromodeoxyuridine (Sigma, 1 mg/mouse) was administered intraperitoneally twice a day for 3 days. Cell populations identified by surface staining were sorted in a FACS-Vintage (Becton Dickinson). Labeling with fluorescein isothiocyanate (FITC)-anti-BrdU mAb (Becton Dickinson) was done as described (10).
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    • unpublished data
    • We used this system to immunize Tg cells rather than directly stimulating Tg female mice with male cells to generate homogeneous populations of activated Tg cells. In fact, when female Tg mice are immunized with male cells, a substantial fraction of Tg lymphocytes is never activated by male antigen (B. Rocha, unpublished data).
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    • note
    • + cells persist in these hosts.
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    • note
    • b knockout mice were constructed.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.