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Volumn 283, Issue 5402, 1999, Pages 676-679

Coordinated regulation of iron-controlling genes, H-ferritin and IRP2, by c-MYC

Author keywords

[No Author keywords available]

Indexed keywords

FERRITIN; IRON REGULATORY FACTOR; MYC PROTEIN; TRANSCRIPTION FACTOR;

EID: 0033613854     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.283.5402.676     Document Type: Article
Times cited : (293)

References (47)
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    • The H-ferritin probe was generated by reverse transcriptase (RT)-PCR amplification of U937 RNA. For the c-MYC exons 1 and 3, probes were obtained from the pMC41RC and pMC41ER plasmids [R. Dalla-Favera et al., Proc. Natl. Acad. Sci. U.S.A. 79, 6497 (1982)]. The SV40 probe was obtained by digesting the pHeBoCMVFerritin vector with the Bam HI and Sal I enzymes to generate a 400-base pair fragment. The 12S ribosomal RNA probe was obtained by RT-PCR from U937 RNA.
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    • The human and rat IRP2 probes were generated by RT-PCR amplification of mRNAs from human lymphoblastoid cells and Rat1 cells, respectively. The primers used for the generation of the IRP2 probe were 5′-CCATAGCAGGCACAGTGAAT and 3′-GGAATCTGCATTTTCTCCTG (both identical for human and rat).
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    • note
    • We thank B. Vogelstein, R. Eisenman, T. Littlewood, F. Costanzo, G. Bornkamm, P. Arosio, J. Sedivy, T. McGraw, E. Ziff, R. Liem, and E. Leibold for providing various reagents and cell lines and E. Marcantonio, B. Tycko, and A. Migliazza for critical reading of the manuscript. K.J.W. is a Fellow of the Leukemia Society of America. Supported by NIH grant CA-37165.


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