Properties of excitatory amino acid transport in the human U373 astrocytoma cell line

Brain Research

Volumn 839, Issue 2, 1999, Pages 235-242

  Dunlop, John ^a   Lou, Zhuangwei ^a   McIlvain, H Beal ^a  

^a PFIZER INC   (United States)

Author keywords

EAAT; Excitatory amino acid transporter; Human astrocytoma; L Glutamate transport

Indexed keywords

2 AMINOADIPIC ACID; 3 HYDROXYASPARTIC ACID; ASPARTIC ACID; ASPARTIC ACID DERIVATIVE; BISINDOLYLMALEIMIDE; CARRIER PROTEIN; CYSTEIC ACID; CYSTEINE SULFINIC ACID; DEXTRO ASPARTIC ACID; DIHYDROKAINIC ACID; EXCITATORY AMINO ACID; GLUTAMIC ACID; GLUTAMIC ACID DERIVATIVE; GLYCINE DERIVATIVE; KAINIC ACID; PYRROLIDINE DERIVATIVE; RECEPTOR SUBTYPE; SODIUM;

AMINO ACID TRANSPORT; ARTICLE; ASTROCYTOMA; BINDING AFFINITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MACROGLIA; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SODIUM CELL LEVEL; TRANSPORT KINETICS;

AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; ASTROCYTOMA; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; EXCITATORY AMINO ACID TRANSPORTER 1; EXCITATORY AMINO ACID TRANSPORTER 2; EXCITATORY AMINO ACID TRANSPORTER 3; GENE EXPRESSION REGULATION, NEOPLASTIC; GLUTAMATE PLASMA MEMBRANE TRANSPORT PROTEINS; GLUTAMIC ACID; HUMANS; RATS; RECEPTORS, GLUTAMATE; RECEPTORS, NEUROTRANSMITTER; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SODIUM; SYMPORTERS; TRANSCRIPTION, GENETIC; TRITIUM; TUMOR CELLS, CULTURED;

EID: 0033612892     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(99)01714-X     Document Type: Article

References (49)

1. Arias-Montano J.A., Berger V., Young J.M. Calcium-dependence of histamine- and carbachol-induced inositol phosphate formation in human U373 MG astrocytoma cells: comparison with HeLa cells and brain slices. Br. J. Pharmacol. 111:1994;598-608.
2. Arriza J.L., Fairman W.A., Wadiche J.I., Murdoch G.H., Kavanaugh M.P., Amara S.G. Functional comparisons of three glutamate transporter subtypes cloned from human cortex. J. Neurosci. 14:1994;5559-5569.
3. Arriza J.L., Eliasof S., Kavanaugh M.P., Amara S.G. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. U.S.A. 94:1997;4155-4160.
4. Bridges R.J., Stanley M.S., Anderson M.W., Cotman C.W., Chamberlin A.R. Conformationally defined neurotransmitter analogues. Selective inhibition of glutamate uptake by one pyrrolidine-2,4-dicarboxylate diastereomer. J. Med. Chem. 34:1991;717-725.
5. Bridges R.J., Lovering F.E., Koch H., Cotman C.W., Chamberlin A.R. A conformationally constrained competitive inhibitor of the sodium-dependent glutamate transporter in forebrain synaptosomes: L-anti-endo-3,4-methanopyrrolidine dicarboxylate. Neurosci. Lett. 174:1994;193-197.
6. Camby I., Salmon I., Oiry C., Galleyrand J.C., Nagy N., Danguy A., Brotchi J., Pasteels J.L., Martinez J., Kiss R. The influence of gastrin and/or cholecystokinin antagonists on the proliferation of three human astrocytic tumor cell lines. Neuropeptides. 30:1996;433-437.
7. Casado M., Bendahan A., Zafra F., Danbolt N.C., Aragon C., Gimenez C., Kanner B.I. Phosphorylation and modulation of brain glutamate transporters by protein kinase C. J. Biol. Chem. 268:1993;27313-27317.
8. 50) of an enzymatic reaction. Biochem. Pharmacol. 22:1973;3099-3108.
9. Conrad M., Stoffel W. Inhibition of the high-affinity brain glutamate transporter GLAST-1 via direct phosphorylation. J. Neurochem. 68:1997;1244-1251.
10. Conti F., DeBiasi S., Minelli A., Rothstein J.D., Melone M. EAAC1, a high-affinity glutamate transporter localised to astrocytes and GABAergic neurones besides pyramidal cells in the rat cerebral cortex. Cerebral Cortex. 8:1998;108-116.
11. Danbolt N.C., Pines G., Kanner B.I. Purification and reconstitution of the sodium- and potassium-coupled glutamate transport glycoprotein from rat brain. Biochemistry. 29:1990;6734-6740.
12. +-dependent L-glutamate transport activity in C6 glioma cells by phorbol ester. J. Neurochem. 67:1996;508-516.
13. +-dependent glutamate transport activity in synaptosomes, C6 glioma and Xenopus oocytes expressing excitatory amino acid carrier 1 (EAAC1). Mol. Pharmacol. 49:1996;465-473.
14. Dunlop J., McIlvain H.B., Lou Z., Franco R. The pharmacological profile of L-glutamate transport in human NT2 neurones is consistent with excitatory amino acid transporter 2. Eur. J. Pharmacol. 360:1998;249-256.
15. Dunlop J., Lou Z., Zhang Y., Kadakia N., Briggs-Schultz H., Zaleska M.M. Pharmacological characterisation of the human glutamate transporters EAAT1 and EAAT2 expressed in mammalian cell lines (Abstr.). J. Neurochem. 70:1998;S69B. Suppl. 1.
16. Fairman W.A., Vandenberg R.J., Arriza J.L., Kavanaugh M.P., Amara S.G. An excitatory amino-acid transporter with properties of a ligand-gated chloride channel. Nature. 375:1995;599-603.
17. Fiebich B.L., Biber K., Gyufko K., Berger M., Bauer J., van Calker D. Adenosine A2b receptors mediate an increase in interleukin (IL)-6 mRNA and IL-6 protein synthesis in human astroglioma cells. J. Neurochem. 66:1996;1426-1431.
18. Furuta A., Rothstein J.D., Martin L.J. Glutamate transporter protein subtypes are expressed differentially during rat CNS development. J. Neurosci. 17:1997;8363-8375.
19. Ganel R., Crosson C.E. Modulation of human glutamate transporter activity by phorbol ester. J. Neurochem. 70:1998;993-1000.
20. 3H ]glutamate transport in glial cultures J. Neurochem. 64:1995;2572-2580.
21. Gegelashvili G., Civenni G., Racagni G., Danbolt N.C., Schousboe I., Schousboe A. Glutamate receptor agonists up-regulate glutamate transporter GLAST in astrocytes. NeuroReport. 8:1996;261-265.
22. Gegelashvili G., Danbolt N.C., Schousboe A. Neuronal soluble factors differentially regulate the expression of the GLT1 and GLAST glutamate transporters in cultured astroglia. J. Neurochem. 69:1997;2612-2615.
23. Gegelashvili G., Schousboe A. High affinity glutamate transporters: regulation of expression and activity. Mol. Pharmacol. 52:1997;6-15.
24. Hussain M.M., Zannis V.I., Plaitakis A. Characterization of glutamate dehydrogenase isoproteins purified from the cerebellum of normal subjects and patients with degenerative neurological disorders, and from human neoplastic cell lines. J. Biol. Chem. 264:1989;20730-20735.
25. Kanai Y., Hediger M.A. Primary structure and functional characterisation of a high-affinity glutamate transporter. Nature. 360:1992;467-471.
26. Kanai Y., Smith C.P., Hediger M.A. The elusive transporters with a high affinity for glutamate. Trends Neurosci. 16:1993;365-370.
27. Kanai Y., Stelzner M., Nussberger S., Khawaja S., Hebert S.C., Smith C.P., Hediger M.A. The neuronal and epithelial human high affinity glutamate transporter. Insights into structure and mechanism of transport. J. Biol. Chem. 269:1994;20599-20606.
28. Kanner B.I. Glutamate transporters from brain. A novel neurotransmitter transporter family. FEBS Lett. 325:1993;95-99.
29. Klegeris A., Walker D.G., McGeer P.L. Regulation of glutamate in cultures of human monocytic THP-1 and astrocytoma U-373 MG cells. J. Neuroimmunol. 78:1997;152-161.
30. Mennerick S., Dhond R.P., Benz A., Xu W., Rothstein J.D., Danbolt N.C., Isenberg K.E., Zorumski C.F. Neuronal expression of the glutamate transporter GLT-1 in hippocampal microcultures. J. Neurosci. 18:1998;4490-4499.
31. Murphy G.M. Jr., Lee Y.L., Jia X.C., Yu A.C., Majewska A., Song Y., Schmidt K., Eng L.F. Tumor necrosis factor-alpha and basic fibroblast growth factor decrease glial fibrillary acidic protein and its encoding mRNA in astrocyte cultures and glioblastoma cells. J. Neurochem. 65:1995;2716-2724.
32. Nagao S., Kwak S., Kanazawa I. EAAT4, a glutamate transporter with properties of a chloride channel, is predominantly localized in Purkinje cell dendrites, and forms parasagittal compartments in rat cerebellum. Neuroscience. 78:1997;929-933.
33. Nakamura Y., Kataoka K., Ishida M., Shinozaki H. (2S,3S,4R)-2-(carboxycyclopropyl)glycine, a potent and competitive inhibitor of both glial and neuronal uptake of glutamate. Neuropharmacology. 32:1993;833-837.
34. Palos T.P., Ramachandran B., Boado R., Howard B.D. Rat C6 and human astrocytic tumor cells express a neuronal type of glutamate transporter. Mol. Brain Res. 37:1996;297-303.
35. Pines G., Danbolt N.C., Bjoras M., Zhang Y., Bendahan A., Eide L., Koepsell H., Storm-Mathisen J., Seeberg E., Kanner B.I. Cloning and expression of a rat brain L-glutamate transporter. Nature. 360:1992;464-467.
36. 3H ]glutamate transport processes in rat brain Brain Res. 544:1991;196-202.
37. 3H ]glutamate transport activity: pharmacologic specificity and regulation by sodium and potassium J. Neurochem. 60:1993;167-179.
38. Robinson M.B., Dowd L.A. Heterogeneity and functional properties of subtypes of sodium-dependent glutamate transporters in the mammalian central nervous system. Adv. Pharmacol. 37:1997;69-115.
39. Rothstein J.D., Martin L., Levey A.I., Dykes-Hoberg M., Jin L., Wu D., Nash N., Kuncl R.W. Localisation of neuronal and glial glutamate transporters. Neuron. 13:1994;713-725.
40. +-dependent glutamate transporters by cyclic AMP analogs and neurons. Mol. Pharmacol. 53:1998;355-369.
41. 3H ]GABA release from human astrocytoma U373 MG cells Eur. J. Pharmacol. 318:1996;185-192.
42. +-dependent glutamate/aspartate transporter from rat brain. Proc. Natl. Acad. Sci. U.S.A. 89:1992;10955-10959.
43. Swanson R.A., Liu J., Miller J.W., Rothstein J.D., Farrell K., Stein B.A., Longuemare M.C. Neuronal regulation of glutamate transporter subtype expression in astrocytes. J. Neurosci. 17:1997;932-940.
44. Takeda Y., Chou K.B., Takeda J., Sachais B.S., Krause J.E. Molecular cloning, structural characterization and functional expression of the human substance P receptor. Biochem. Biophys. Res. Commun. 179:1991;1232-1240.
45. Ullensvang K., Lehre K.P., Storm-Mathisen J., Danbolt N.C. Differential developmental expression of the two rat brain glutamate transporter proteins GLAST and GLT. Eur. J. Neurosci. 9:1997;1646-1655.
46. Velaz-Faircloth M., McGraw T.S., Alandro M.S., Fremeau R.T. Jr., Kilberg M.S., Anderson K.J. Characterization and distribution of the neuronal glutamate transporter EAAC1 in rat brain. Am. J. Physiol. 270:1996;C67-C75.
47. Wang G.J., Chung H.J., Schnuer J., Pratt K., Zable A.C., Kavanaugh M.P., Rosenberg P.A. High affinity glutamate transport in rat cortical neurones in culture. Mol. Pharmacol. 53:1998;88-96.
48. Watkins J.C., Evans R.H. Excitatory amino acid transmitters. Annu. Rev. Pharmacol. Toxicol. 21:1981;165-204.
49. Yamada K., Watanabe M., Shibata T., Tanaka K., Wada K., Inoue Y. EAAT4 is a post-synaptic glutamate transporter at Purkinje cell synapses. NeuroReport. 7:1996;2013-2017.