Reverse action of ribonuclease T1 in frozen aqueous systems

Biological Chemistry

Volumn 378, Issue 2, 1997, Pages 115-118

  Haensler, Marion ^a   Hahn, Ulrich ^a   Jakubke, Hans Dieter ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Dinucleoside monophosphate synthesis; Enzymatic synthesis; Frozen systems; RNase T1

Indexed keywords

CYTIDINE; NUCLEOSIDE ANALOG; RIBONUCLEASE T1;

AQUEOUS SOLUTION; ARTICLE; CHEMICAL BOND; CONTROLLED STUDY; ENZYME MECHANISM; ESTERIFICATION; FREEZING; HYDROLYSIS; PRIORITY JOURNAL;

DINUCLEOSIDE PHOSPHATES; FREEZING; KINETICS; RIBONUCLEASE T1; WATER;

EID: 0030955160     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. 1 variants leads to an improved scheme for the reaction mechanism. Biochem. Biophys. Res. Commun. 199, 213-219.
2. Bongers, J., and Heimer, E.P. (1994). Recent applications of enzymatic peptide synthesis. Peptides 15, 183-193.
3. Eigen, M., and de Mayer, L. (1958). Self-dissociation and protonic charge transport in water and ice. Proc. Roy. Soc. (London) A 247, 505-533.
4. Georgalis, Y., Zouni, A., Hahn, U., and Saenger, W. (1991). Synthesis and kinetic study of transition state analogs for ribonuclease T1. Biochim. Biophys. Acta 1118, 1-5.
5. Grunert, H.-P., Landt, O., Zirpel-Giesebrecht, M., Bachmann, J., Heinemann, U., Saenger, W. and Hahn, U. (1993). Trp59 to Tyr substitution enhances the catalytic activity of RNase T1 and of the Tyr to Trp variants in positions 24, 42 and 45. Protein Engineering 6, 739-744.
6. Haensler, M., and Jakubke, H.-D. (1996a). Nonconventional protease catalysis in frozen aqueous solutions. J. Peptide Sci. 2, 279-289.
7. Haensler, M., and Jakubke, H.-D. (1996b). Reverse action of hydrolases in frozen aqueous solutions. Amino Acids 11, 379-395.
8. Heinemann, U., and Saenger, W. (1982). Specific protein-nucleic acid recognition in ribonuclease T1-2′-guanylic acid complex: an X-ray study. Nature 299, 27-31.
9. Jakubke, H.-D. (1994). Protease-catalysed peptide synthesis. Basic principles, new synthesis strategies and medium engineering. J. Chin. Chem. Soc. 41, 355-370.
10. Landt, O., Zirpel-Giesebrecht, M., Milde, A., and Hahn, U. (1992). Improving purification of recombinant ribonuclease T1. J. Biotechnol. 24, 189-194.
11. Mohr, S.C., and Thach, R.E. (1969). Application of ribonuclease T1 to the synthesis of oligoribonucleotides of defined base sequence. J. Biol. Chem. 244, 6566-6576.
12. Rowe, M.J., and Smith, M.A. (1970). Synthesis of 3′-5′-dinucleotides with RNase T1. Biochem. Biophys. Res. Commun. 38, 393-399.
13. Rowe, M.J., and Smith, M.A. (1972). Synthesis of oligoribonucleotides with ribonuclease T1. Biochim. Biophys. Acta 281, 338-346.
14. Pincock, R.E., and Kiovsky, T.E. (1966). Kinetics of reactions in frozen solutions. J. Chem. Educ. 43, 358-360.
15. Strambini, G.B., and Gabellieri, E. (1996). Proteins in frozen solutions: Evidence of ice-induced partial unfolding. Biophys. J. 70, 971-976.
16. William-Smith, D.L., Bray, R.C., Barber, M.J., Tsopanakis, A.D., and Vincent, S.P. (1977). Changes in apparent pH on freezing aqueous buffer solutions and their relevance to biochemical electron-paramagnetic -resonance spectroscopy. Biochem. J. 167, 593-600.