Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine

Journal of Bacteriology

Volumn 179, Issue 13, 1997, Pages 4446-4450

  Otten, Sharee L ^a   Gallo, Mark A ^a,b   Madduri, Krishnamurthy ^a,c   Liu, Xiaochun ^a   Hutchinson, C Richard ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b NIAGARA UNIVERSITY   (United States)

^c DOW AGROSCIENCES LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DAUNORUBICIN;

AMINO ACID SEQUENCE; ARTICLE; DNA SEQUENCE; GENE DISRUPTION; GENE REPRESSION; GENETIC ANALYSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STREPTOMYCES;

STREPTOMYCES; STREPTOMYCES PEUCETIUS;

EID: 0030794691     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.13.4446-4450.1997     Document Type: Article

References (33)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Amann, E., B. Ochs, and K.-J. Abel. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69:301-315.
3. Bierman, M., R. Logan, K. O'Brien, E. T. Seno, R. Nagaraja Rao, and B. E. Schoner. 1992. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43-49.
4. Black, P. N., and C. C. DiRusso. 1994. Molecular and biochemical analyses of fatty acid transport, metabolism, and gene regulation in Escherichia coli. Biochim. Biophys. Acta 1210:123-145.
5. Buendia, A. M., B. Enenkel, R. Koplin, K. Niehaus, W. Arnold, and A. Puhler. 1991. The Rhizobium meliloti exoZl exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM. Mol. Microbiol. 5:1519-1530.
6. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
7. Fujiwara, A., and T. Hoshino. 1983. Anthracyclic antibiotics. Crit. Rev. Biotechnol. 3:133-157.
8. Gallo, M. A., J. Ward, and C. R. Hutchinson. 1996. The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster. Microbiology 142:269-275.
9. Guilfoile, P. G., and C. R. Hutchinson. 1991. A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. Proc. Natl. Acad. Sci. USA 88:8553-8557.
10. Hillemann, D., A. Puhler, and W. Wohlleben. 1991. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Res. 19:727-731.
11. Hopwood, D. A., M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, C. P. Smith, J. M. Ward, and H. Schrempf. 1985. Genetic manipulation of Streptomyces: a laboratory manual. The John Innes Foundation, Norwich, United Kingdom.
12. Krugel, H., G. Schumann, F. Hanel, and G. Fiedler. 1993. Nucleotide sequence analysis of five putative Streptomyces griseus genes, one of which complements an early function in daunorubicin biosynthesis that is linked to a putative gene cluster involved in TDP-daunosamine formation. Mol. Gen. Genet. 241:193-202.
13. Landini, P, L. I. Hajec, and M. R. Volkert. 1994. Structure and transcriptional regulation of the Escherichia coli adaptive response gene aidB. J. Bacteriol. 176:6583-6589.
14. Liu, H.-W., and J. S. Thorson. 1994. Pathways and mechanisms in the biogenesis of novel deoxysugars by bacteria. Annu. Rev. Microbiol. 48:223-256.
15. MacNeil, D. Personal communication.
16. Macpherson, D. F., P. A. Manning, and R. Morona. 1994. Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri. Mol. Microbiol. 11:281-292.
17. 1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. J. Bacteriol. 177:1208-1215.
18. Matsubara, Y., Y. Indo, E. Naito, H. Ozasa, R. Glassberg, J. Vockley, Y. Ikeda, J. Kraus, and K. Tanaka. 1989. Molecular cloning and nucleotide sequence of cDNAs encoding the precursors of rat long chain acyl-coenzyme A, short chain acyl-coenzyme A, and isovaleryl-coenzyme A dehydrogenases. J. Biol. Chem. 264:16321-16331.
19. Naito, E., H. Ozasa, Y. Ikeda, and K. Tanaka. 1989. Molecular cloning and nucleotide sequence of complementary DNAs encoding human short chain acyl-coenzyme A dehydrogenase and the study of the molecular basis of human short chain acyl-coenzyme A dehydrogenase deficiency. J. Clin. Invest. 83:1989.
20. 2 locus. J. Bacteriol. 177:1216-1224.
21. Otten, S. L., X. Liu, J. Ferguson, and C. R. Hutchinson. 1995. Cloning and characterization of the Streptomyces peucetius dnrQS genes encoding a daunosamine biosynthesis enzyme and a glycosyl transferase involved in daunorubicin biosynthesis. J. Bacteriol. 177:6688-6692.
22. Pissowotzki, K., K. Mansouri, and W. Piepersberg. 1991. Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative function of genes strELMB2N. Mol. Gen. Genet. 231:113-123.
23. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Scotti, C., and C. R. Hutchinson. 1996. Enhanced antibiotic production by manipulation of the Streptomyces peucetius dnrH and dnmT genes involved in doxorubicin (adriamycin) biosynthesis. J. Bacteriol. 178:7316-7321.
25. Stutzman-Engwall, K. J., S. L. Otten, and C. R. Hutchinson. 1992 Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. J. Bacteriol. 174:144-154.
26. Summers, R. G., M. J. Staver, S. Donadio, E. Wendt-Pienkowski, C. R. Hutchinson, and L. Katz. Characterization of ten genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production. Microbiology, in press.
27. Tanaka, K., and Y. Ino. 1992. Evolution of the acyl-CoA dehydrogenase/ oxidase superfamily. Prog. Clin. Biol. Res. 375:95-110.
28. Thompson, M. W., W. R. Strohl, and H. G. Floss. 1992. Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes. J. Gen. Microbiol. 138:779-786.
29. Thorson, J. S., S. F. Lo, and H.-W. Liu. 1993. Molecular basis of 3,6-dideoxyhexose biosynthesis: elucidation of CDP-ascarylose biosynthetic genes and their relationship to other 3.6-dideoxyhexose pathways. J. Am. Chem. Soc. 115:5827-5828.
30. Thorson, J. S., S. F. Lo, H.-W. Liu, and C. R. Hutchinson. 1993. Biosynthesis of 3,6-dideoxyhexoses: new mechanistic reflections upon 2.6-dideoxy, 4,6-dideoxy, and amino sugar construction. J. Am. Chem. Soc. 115:6993-6994.
31. Thorson, J. S., S. F. Lo, O. Ploux, X. He, and H.-W. Liu. 1994. Studies of the biosynthesis of 3,6-dideoxyhexoses: molecular cloning and characterization of the use (ascarylose) region from Yersinia pseudotuberculosis serogroup VA. J. Bacteriol. 176:5483-5493.
32. Wierenga, R. K., P. Terpstra, and W. G. J. Hol. 1986. Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint. J. Mol. Biol. 187:101-107.
33. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.