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The solutions, spotted on prechilled parafilm floating on ice water, were irradiated with a glass-filtered long wavelength UVGL-25 lamp (UVP, Inc.) at a distance of ca. 5 cm.
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A Kodak Digital Science DC40 camera in combination with 1D Analysis Software Version 1.6 was used to quantify the bands.
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This was confirmed in a time course experiment with 1.0 mM BISPHEN which showed a higher magnitude of pRNA degradation without the production of additional cross-linked pRNA.
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