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Volumn 284, Issue 5422, 1999, Pages 1985-1988

Preventing neurodegeneration in the Drosophila mutant bubblegum

Author keywords

[No Author keywords available]

Indexed keywords

LORENZO OIL; TRIOLEIN; VERY LONG CHAIN FATTY ACID;

EID: 0033581027     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5422.1985     Document Type: Article
Times cited : (151)

References (25)
  • 4
    • 0344486611 scopus 로고    scopus 로고
    • note
    • +) were screened for mutations causing reduced life-span at 29°C. Candidates showing reduced life-span compared with the parent strain were examined after aging (but before death) to identify those with brain degeneration (3), The bubblegum mutant showed such degeneration and was selected for further characterization.
  • 5
    • 0344917820 scopus 로고    scopus 로고
    • note
    • Fly heads were prepared by fixation in 1% paraformaldehyde plus 1% glutaraldehyde, postfixation in 1% osmium tetroxide, dehydration in an ethanol series, and embedding in Epon 812. For light microscopy, 1-μm sections were stained with 1% toluidine blue plus 1% Borax. For electron microscopy, ultrathin sections (80 nm) were examined with a Philips 201 electron microscope at 60 kV.
  • 6
    • 0344917821 scopus 로고    scopus 로고
    • note
    • The presence of a single insertion of the P-element was confirmed by genomic Southern (DNA) blots. Precise excision of the P-element from the mutant restored the normal phenotype.
  • 10
  • 14
    • 0027532282 scopus 로고
    • J. Messer et al., Nature 361, 726 (1993).
    • (1993) Nature , vol.361 , pp. 726
    • Messer, J.1
  • 16
  • 21
    • 0000064419 scopus 로고
    • Flies were placed in a countercurrent distribution apparatus and tested repeatedly for response to light [S. Benzer, Proc. Natl. Acad. Sci. U.S.A. 58, 1112 (1967)]. The abscissa represents the number of positive responses toward light in a total of five trials. In control tests for the from-light response, neither group of flies moved away from light.
    • (1967) Proc. Natl. Acad. Sci. U.S.A. , vol.58 , pp. 1112
    • Benzer, S.1
  • 25
    • 0344917816 scopus 로고    scopus 로고
    • note
    • We thank R. Young, C. Kang, and A. Gomez for expert technical support and members of our research group for helpful discussions. Supported by a postdoctoral fellowship from the Dell Webb Foundation (K.-T.M.) and by grants (S.B.) from NSF, NIH, and the James G. Boswell Foundation.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.