Resin-supported labeling reagents

Bioorganic and Medicinal Chemistry Letters

Volumn 9, Issue 2, 1999, Pages 217-220

  Adamczyk, Maciej ^a   Fishpaugh, Jeffrey R ^a   Mattingly, Phillip G ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACRIDINE ORANGE; BIOTIN; COUMARIN; DESIPRAMINE; ESTRIOL; FLUORESCEIN; PHENYTOIN; RESIN; THYROXINE;

ARTICLE; DRUG DETERMINATION; FLUORESCENCE;

EID: 0033579915     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(98)00711-2     Document Type: Article

References (19)

1. Wild, D. The Immunoassay Handbook; Stockton Press: New York, 1994.
2. Hermanson, G. T. Bioconjugate Techniques; Academic: San Diego, 1996.
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4. Adamczyk, M.; Fishpaugh, J. R.; Mattingly, P. G. Tetrahedron Lett. In press.
5. Adamczyk, M.; Fishpaugh, J.; Heuser, K. Bioconjugate Chem. 1997, 8, 253.
6. NHS esters can also be purchased from Molecular Probes, Eugene, OR.
7. Adamczyk, M.; Chen, Y.-Y.; Moore, J. A.; Mattingly, P. G. Bioorg. Med. Chem. Lett. 1998, 8, 1281.
8. Adamczyk, M.; Johnson, D. D.; Mattingly, P. G.; Moore, J. A.; Pan, Y. Bioconjugate Chem. 1998, 9, 23.
9. Adamczyk, M.; Mattingly, P. G.; Reddy, R. E. Steroids 1998, 63, 130.
10. Adamczyk, M.; Chen, Y. Y.; Mattingly, P. G. Bioorg. Med. Chem. Lett. 1998, 8, 1281.
11. Wilchek, M.; Bayer, E. A. Methods in Enzymol. 1990, 184, 123.
12. Adamczyk, M.; Chen, Y.-Y.; Mattingly, P. G.; Pan, Y. J. Org. Chem. 1998, 63, 5636.
13. Hapten 11 was prepared from estradiol-6-CMO (Steraloids, Wilton, NH): (a) EDC/NHS; (b) 1,8-diaminooctane.
14. Flentge, C. A.; Kirkemo, C. L. Eur. Patent Appl. EP 108403 A2 840516.
15. Adamczyk, M.; Fishpaugh, J. R.; Gebler, J. C.; Mattingly, P. G.; Shreder, K. Eur. Mass Spec. 1998, 4, 121.
16. Biwersi, J.; Tulk, B.; Verkman, A. S. Anal. Biochem. 1994, 219, 139.
17. Hapten 13 was prepared from 5,5-diphenylhydantoin (Aldrich): (a) KOH/EtOH; (b) 1,2-dibromoethane; (c) ammonium hydroxide.
18. 18 column, eluting with acetonitrile/0.05% aq trifluoroacetic acid with UV detection at 254 nm.]
19. The major impurity was residual solvent. Minor impurities sometimes carried over from the starting amine. No active ester resin decomposition products were seen.