Preparation of succinimidyl and pentafluorophenyl active esters of 5- and 6- carboxyfluorescein

Bioconjugate Chemistry

Volumn 8, Issue 2, 1997, Pages 253-255

  Adamczyk, Maciej ^a   Fishpaugh, Jeffrey R ^a   Heuser, Kevin J ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MIXTURES;

ACTIVE ESTERS; CARBOXYFLUORESCEIN; N-HYDROXYSUCCINIMIDE; REGIOISOMERIC MIXTURES;

ESTERS;

EID: 0031106153     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc9600877     Document Type: Article

References (19)

1. Haughland, R. P. (1991) Biosensors with Fiberoptics (D. L. Wise and D. Wingard, Eds.) p 85-109, Humana Press, Clifton, NJ.
2. Bright, F. V. (1988) Bioanalytical Applications of Fluorescence Spectroscopy. Anal. Chem. 60, 1031A-1039A.
3. Owens, M. A., and Loken, M. R. (1995) Flow Cytometry: Principles for Clinical Laboratory Practice, pp 28-29, Wiley-Liss, New York.
4. Haughland, R. P. (1992) Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Eugene, OR.
5. Miki, M. (1987) The Recovery of the Polymerizability of Lys-61-Labelled Actin by the Addition of Phalloidin. Eur. J. Biochem. 164, 229-235.
6. Lu-Steffes, M., et al. (1982) Fluorescence Polarization Immunoassay IV. Determination of Phenytoin and Phenobarbital in Human Serum and Plasma. Clin. Chem. 28, 2278-2282.
7. Adamczyk, M., Harrington, C., and Johnson, D. (1994) Reagents and Methods for the Quantification of Imipramine and Desipramine in Biological Fluids. U.S. Pat. 5,340,750.
8. Bodanszky, M. (1979) The Peptides. Vol. 1, pp 106-175, Academic Press, New York.
9. Bodanszky, M. (1984) Principles of Peptide Synthesis, pp 28-49, Springer-Verlag, New York.
10. Reference 4, p 26.
11. 3CN/20% MeOH/60% aqueous 0.1% formic acid.
12. Molecular Probes, Eugene, OR. 1996 price $380 per 50 mg, 20 × $380/50 mg = $7600/g.
13. Yoshida, R. A. (1980) Support Ligand Analog Fluorescer Conjugate and Serum Assay Method Involving Such Conjugate. Eur. Pat. Appl. 0015695.
14. Vanderbilt, A. S., Osikowicz, E. W., Fino, J. R., and Shipchandler, M. T. (1986) Total Estriol Fluorescence Polarization Immunoassay. Eur. Pat. Appl. 0200960.
15. Imming, P., and Jung, M-H. (1995) Pentafluorophenyl Esters of Dicarboxylic Acids. Arch. Pharm. 328, 87-91.
16. Stainless steel sample injection module (SIM) which delivers sample onto the cartridge.
17. Eluted with 15% acetone/85% toluene containing 0.05% acetic acid.
18. Eluted with 70% ethyl acetate/30% toluene (6000 mL), then 90% ethyl acetate/10% toluene (4000 mL), then 100% acetone containing 0.1% acetic acid (2000 mL).
19. Biotage FLASH 75 system user's manual, version 2.0 (1995).