Mitochondrial and chloroplast phage-type RNA polymerases in Arabidopsis

Science

Volumn 277, Issue 5327, 1997, Pages 809-811

  Hedtke, Boris ^a   Börner, Thomas ^a   Weihe, Andreas ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARABIDOPSIS; ARTICLE; BACTERIOPHAGE; CHLOROPLAST; ENZYME ANALYSIS; GENE DUPLICATION; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ARABIDOPSIS; CELL NUCLEUS; CHLOROPLASTS; CLONING, MOLECULAR; DNA-DIRECTED RNA POLYMERASES; EXONS; GENES, PLANT; INTRONS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; PHYLOGENY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; T-PHAGES;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS); EUKARYOTA; UNIDENTIFIED BACTERIOPHAGE;

EID: 1842411934     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5327.809     Document Type: Article

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22. Chloroplasts were isolated from a commercial cultivar of spinach (Spinacia oleracea L.). Organelle isolation and in vitro import followed the protocol of B. D. Bruce, S. Perry, J. Froehlich, and K. Keegstra [in Plant Molecular Biology Manual, S. B. Gelvin and R. A. Schilperpoort, Eds. (Kluwer Academic, Dordrecht, Netherlands, 1994), pp. J1, 1-15]. Import reactions consisted of 62 μl of import buffer, 3 μl of 100 mM adenosine-5′-triphosphate, 10 μl of in vitro translation mix, and 25 μl of purified chloroplasts (equivalent to a chlorophyll concentration of 1 mg/ml). Import was allowed to take place for 30 min at room temperature. Thermolysin (Sigma) treatment (0.2 mg/ml) was performed after import for 30 min on ice.
23. We thank J. Haseloff for the vector pBIN-mGFP5-ER; the Arabidopsis DNA Stock Centre (Max Planck Institut für Züchtungsforschung, Köln, Germany) for the genomic A. thaliana library; M. Boutry for a ATPase β presequence clone; R. B. Klösgen and R. Lührs for waxy constructs; C. Stock, I. Wagner, and J. Müller for technical assistance; W. Schuster, Berlin, for sharing unpublished data of an Arabidopsis DNA sequence [accession number Y09432 (W. Schuster et al., unpublished data)] that is nearly identical to clone RpoY; and B. Neilan for critical reading of the manuscript. Part of this work was funded by grants from the Deutsche Forschungsgemeinschaft and the Fond der Chemischen Industrie to T.B.