Synthesis of Modified Oligodeoxyribonucleotides on a Solid-Phase Support via Derivatization of a Selectively Revealed 2′-Amino-2′-deoxyuridine

Organic Letters

Volumn 1, Issue 12, 1999, Pages 2021-2024

  Hwang, Jae Taeg ^a   Greenberg, Marc M ^a  

^a Veterinary Dentistry and Oral Surgery   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2' AMINO 2' DEOXYURIDINE; 2'-AMINO-2'-DEOXYURIDINE; DEOXYURIDINE; DRUG DERIVATIVE; OLIGODEOXYRIBONUCLEOTIDE;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOTOLYSIS; SYNTHESIS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DEOXYURIDINE; MASS SPECTROMETRY; OLIGODEOXYRIBONUCLEOTIDES; PHOTOLYSIS;

EID: 0033576422     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol991196e     Document Type: Article

References (23)

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19. See the Supporting Information.
20. 2 (10 mL), collected, and placed in a screw-capped vial for storage.
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22. 3CN and drying under vacuum. The free-flowing resin was treated with 28% aqueous ammonia (600 μL) for 6 h at 55 °C and concentrated under vacuum. Purification of the conjugated oligonucleotide was carried out via 20% polyacrylamide denaturing gel electrophoresis. Isolated yields were obtained (o.d. 260 nm; 350 nm for 5a) by comparing the amount of conjugated oligonucleotide to the amount of unconjugated material subjected to identical deprotection, purification, and isolation conditions.
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