-
1
-
-
0027279421
-
-
A. B. Sachs, Cell 74, 413 (1993).
-
(1993)
Cell
, vol.74
, pp. 413
-
-
Sachs, A.B.1
-
3
-
-
0026337150
-
-
G. Brewer, Mol. Cell. Biol. 11, 2460 (1991); W. Zhang et al., ibid. 13, 7262 (1993).
-
(1991)
Mol. Cell. Biol.
, vol.11
, pp. 2460
-
-
Brewer, G.1
-
4
-
-
0026337150
-
-
G. Brewer, Mol. Cell. Biol. 11, 2460 (1991); W. Zhang et al., ibid. 13, 7262 (1993).
-
(1993)
Mol. Cell. Biol.
, vol.13
, pp. 7262
-
-
Zhang, W.1
-
5
-
-
0344487612
-
-
Y. Kajita, J.-I. Nakayama, M. Aizawa, F. Ishikawa, J. Biol. Chem. 270, 23167 (1995); B. Wagner, C. T. DeMaria, Y. Sun, G. M. Wilson, G. Brewer, genomics 48, 105 (1998).
-
(1995)
J. Biol. Chem.
, vol.270
, pp. 23167
-
-
Kajita, Y.1
Nakayama, J.-I.2
Aizawa, M.3
Ishikawa, F.4
-
6
-
-
0345349992
-
-
Y. Kajita, J.-I. Nakayama, M. Aizawa, F. Ishikawa, J. Biol. Chem. 270, 23167 (1995); B. Wagner, C. T. DeMaria, Y. Sun, G. M. Wilson, G. Brewer, genomics 48, 105 (1998).
-
(1998)
Genomics
, vol.48
, pp. 105
-
-
Wagner, B.1
DeMaria, C.T.2
Sun, Y.3
Wilson, G.M.4
Brewer, G.5
-
7
-
-
0029942784
-
-
A. Pende et al., J. Biol. Chem. 271, 8493 (1996); C. T. DeMaria and G. Brewer, Ibid., p. 12179; O. I. Sirenko et al., Mol. Cell. Biol. 17, 3898 (1997).
-
(1996)
J. Biol. Chem.
, vol.271
, pp. 8493
-
-
Pende, A.1
-
8
-
-
15844383918
-
-
A. Pende et al., J. Biol. Chem. 271, 8493 (1996); C. T. DeMaria and G. Brewer, Ibid., p. 12179; O. I. Sirenko et al., Mol. Cell. Biol. 17, 3898 (1997).
-
J. Biol. Chem.
, pp. 12179
-
-
Demaria, C.T.1
Brewer, G.2
-
9
-
-
0030978801
-
-
A. Pende et al., J. Biol. Chem. 271, 8493 (1996); C. T. DeMaria and G. Brewer, Ibid., p. 12179; O. I. Sirenko et al., Mol. Cell. Biol. 17, 3898 (1997).
-
(1997)
Mol. Cell. Biol.
, vol.17
, pp. 3898
-
-
Sirenko, O.I.1
-
10
-
-
0025177186
-
-
A. Wodner-Filipowicz and C. Moroni, Proc. Natl. Acad. Sci. U.S.A. 87, 777 (1990); C. Y. Chen. F. Del Gatto-Konczak, Z. Wu, M. Karin, Science 280, 1945 (1998); N. Klein, A. M. Curatola, R. J. Schneider, Gene Expr., in press.
-
(1990)
Proc. Natl. Acad. Sci. U.S.A.
, vol.87
, pp. 777
-
-
Wodner-Filipowicz, A.1
Moroni, C.2
-
11
-
-
0032546972
-
-
A. Wodner-Filipowicz and C. Moroni, Proc. Natl. Acad. Sci. U.S.A. 87, 777 (1990); C. Y. Chen. F. Del Gatto-Konczak, Z. Wu, M. Karin, Science 280, 1945 (1998); N. Klein, A. M. Curatola, R. J. Schneider, Gene Expr., in press.
-
(1998)
Science
, vol.280
, pp. 1945
-
-
Chen, C.Y.1
Gatto-Konczak, F.D.2
Wu, Z.3
Karin, M.4
-
12
-
-
0025177186
-
-
in press
-
A. Wodner-Filipowicz and C. Moroni, Proc. Natl. Acad. Sci. U.S.A. 87, 777 (1990); C. Y. Chen. F. Del Gatto-Konczak, Z. Wu, M. Karin, Science 280, 1945 (1998); N. Klein, A. M. Curatola, R. J. Schneider, Gene Expr., in press.
-
Gene Expr.
-
-
Klein, N.1
Curatola, A.M.2
Schneider, R.J.3
-
13
-
-
0030821226
-
-
W. J. Ma, S. Chung, H. Furneaux, Nucleic Acids Res. 25, 3564 (1997); X. C. Fan and J. A. Steitz, EMBO J. 17, 3448 (1998); S. S. Peng, C. Y. Chen, N. Xu, A. B. Shyu, ibid., p. 3460.
-
(1997)
Nucleic Acids Res.
, vol.25
, pp. 3564
-
-
Ma, W.J.1
Chung, S.2
Furneaux, H.3
-
14
-
-
0032526427
-
-
W. J. Ma, S. Chung, H. Furneaux, Nucleic Acids Res. 25, 3564 (1997); X. C. Fan and J. A. Steitz, EMBO J. 17, 3448 (1998); S. S. Peng, C. Y. Chen, N. Xu, A. B. Shyu, ibid., p. 3460.
-
(1998)
EMBO J.
, vol.17
, pp. 3448
-
-
Fan, X.C.1
Steitz, J.A.2
-
15
-
-
0030821226
-
-
W. J. Ma, S. Chung, H. Furneaux, Nucleic Acids Res. 25, 3564 (1997); X. C. Fan and J. A. Steitz, EMBO J. 17, 3448 (1998); S. S. Peng, C. Y. Chen, N. Xu, A. B. Shyu, ibid., p. 3460.
-
EMBO J.
, pp. 3460
-
-
Peng, S.S.1
Chen, C.Y.2
Xu, N.3
Shyu, A.B.4
-
17
-
-
0027399313
-
-
T. Aharon and R. J. Schneider, Mol. Cell. Biol. 13, 1971 (1993); A. M. Curatola, M. Nadal, R. J. Schneider, ibid. 15, 6331 (1995).
-
(1993)
Mol. Cell. Biol.
, vol.13
, pp. 1971
-
-
Aharon, T.1
Schneider, R.J.2
-
18
-
-
0028801330
-
-
T. Aharon and R. J. Schneider, Mol. Cell. Biol. 13, 1971 (1993); A. M. Curatola, M. Nadal, R. J. Schneider, ibid. 15, 6331 (1995).
-
(1995)
Mol. Cell. Biol.
, vol.15
, pp. 6331
-
-
Curatola, A.M.1
Nadal, M.2
Schneider, R.J.3
-
19
-
-
0025836508
-
-
D. M. Koeller, J. A. Horowitz, J. L. Casey, R. D. Klausner, J. B. Harford, Proc. Natl. Acad. Sci. U.S.A. 88, 7778 (1991).
-
(1991)
Proc. Natl. Acad. Sci. U.S.A.
, vol.88
, pp. 7778
-
-
Koeller, D.M.1
Horowitz, J.A.2
Casey, J.L.3
Klausner, R.D.4
Harford, J.B.5
-
20
-
-
0023461273
-
-
M. Moore, J. Schaack, S. B. Baim, R. I. Morimoto, T. Shenk, Mol. Cell. Biol. 7, 45052 (1987).
-
(1987)
Mol. Cell. Biol.
, vol.7
, pp. 45052
-
-
Moore, M.1
Schaack, J.2
Baim, S.B.3
Morimoto, R.I.4
Shenk, T.5
-
21
-
-
0344918721
-
-
note
-
35S]methionine for 4 hours in Dulbecco's modified Eagle's medium without methionine containing 2% bovine serum. Cells were lysed in 1% NP-40, 150 mM NaCl, 20 mM Hepes (pH 7.5), and 2.5 mM EDTA with protease inhibitors (Boehringer-Mannheim Complete), resolved by SDS-PAGE, and visualized by fluorography. β-Galactosidase was immunoprecipitated from equal amounts of protein with antibodies to β-gat (5′-3′ Inc.). Results were quantitated by digital densitometry and are typical of three independent experiments. For indirect immunofluorescence, polyclonal antisera to all AUF1 isoforms (3), mouse monoclonal antibody (mAb) to hsp70 (W-27, Santa Cruz), or mouse α-tubulin mAb (Sigma) were reacted on cover slips. Secondary antibodies were sheep antibody to rabbit immunoglobulin C conjugated with fluorescein (AUFI) and sheep antibody to mouse immunoglobulin G conjugated with rhodamine (hsp70 or α-tubulin). Secondary antibody reacted in the absence of primary antibody showed no staining. Images were acquired on a Zeiss Axiophot photomicroscope.
-
-
-
-
23
-
-
0030871891
-
-
S. Takayama et al., EMBO J. 16, 4887 (1997); M. Zeiner, M. Gebauer, U. Gehring, ibid., p. 5483.
-
(1997)
EMBO J.
, vol.16
, pp. 4887
-
-
Takayama, S.1
-
24
-
-
0030766734
-
-
S. Takayama et al., EMBO J. 16, 4887 (1997); M. Zeiner, M. Gebauer, U. Gehring, ibid., p. 5483.
-
EMBO J.
, pp. 5483
-
-
Zeiner, M.1
Gebauer, M.2
Gehring, U.3
-
25
-
-
0344487608
-
-
note
-
35S]Methionine-labeled HeLa cell lysates (12) from control cells or cells heat-shocked for 4 hours were clarified by centrifugation at 10,000g for 15 min at 4°C AUF1 was immunoprecipitated from equal amounts of protein, resolved by SDS-PAGE, and then fluorographed. AUF1 was immunoprecipitated from equal amounts of unlabeled cell extracts treated with 2 μg of RNase A for 30 min at 30°C. followed by SDS-PAGE and immunoblotting with antibodies specific to hsp70, hsc70, or hsp105 (StressGen), elF4G, PABP, or AUF1 Blots were developed with enhanced chemical luminescence (Amersham). Data represent typical results of three independent experiments. Polysomes were isolated by lysis of HeLa cells (72) (with or without RNase treatment) and centrifugea at 3000g for 2 min at 4°C, then supernatant was centrifuged at 430,000g for 30 min at 4°C in a Beckman TL-100 ultracentrifuge. The polyribosome pellet was collected and resolved by SDS-PAGE.
-
-
-
-
26
-
-
0033521535
-
-
S. Pyronnet et al., EMBO J. 18, 270 (1999).
-
(1999)
EMBO J.
, vol.18
, pp. 270
-
-
Pyronnet, S.1
-
27
-
-
0030898710
-
-
K. T. Bush, A. L. Goldberg, S. K. Nigam, J. Biol. Chem. 272, 9086 (1997); A. Mathew, S. K. Mathur, R. I. Morimoto, Mol. Cell. Biol. 18, 5091 (1998); A. Ciechanover, EMBO J. 17, 7150 (1998).
-
(1997)
J. Biol. Chem.
, vol.272
, pp. 9086
-
-
Bush, K.T.1
Goldberg, A.L.2
Nigam, S.K.3
-
28
-
-
0031841818
-
-
K. T. Bush, A. L. Goldberg, S. K. Nigam, J. Biol. Chem. 272, 9086 (1997); A. Mathew, S. K. Mathur, R. I. Morimoto, Mol. Cell. Biol. 18, 5091 (1998); A. Ciechanover, EMBO J. 17, 7150 (1998).
-
(1998)
Mol. Cell. Biol.
, vol.18
, pp. 5091
-
-
Mathew, A.1
Mathur, S.K.2
Morimoto, R.I.3
-
29
-
-
0030898710
-
-
K. T. Bush, A. L. Goldberg, S. K. Nigam, J. Biol. Chem. 272, 9086 (1997); A. Mathew, S. K. Mathur, R. I. Morimoto, Mol. Cell. Biol. 18, 5091 (1998); A. Ciechanover, EMBO J. 17, 7150 (1998).
-
(1998)
EMBO J.
, vol.17
, pp. 7150
-
-
Ciechanover, A.1
-
30
-
-
0032513215
-
-
A. B. Meriin, V. L. Gabai, J. Yaglom, V. I. Shifrin, M. Y. Sherman, J. Biol. Chem. 273, 6373 (1998).
-
(1998)
J. Biol. Chem.
, vol.273
, pp. 6373
-
-
Meriin, A.B.1
Gabai, V.L.2
Yaglom, J.3
Shifrin, V.I.4
Sherman, M.Y.5
-
31
-
-
0021139080
-
-
D. Finley, A. Ciechanover, A. Varshavsky, Cell 37, 43 (1984); G. Pratt, R. Hough, M. Rechsteiner, J. Biol. Chem. 264, 12526 (1989); B. Bercovich et al., ibid. 272, 9002 (1997); M. Conconi et al., Biochem. J. 333, 407 (1998).
-
(1984)
Cell
, vol.37
, pp. 43
-
-
Finley, D.1
Ciechanover, A.2
Varshavsky, A.3
-
32
-
-
0024323502
-
-
D. Finley, A. Ciechanover, A. Varshavsky, Cell 37, 43 (1984); G. Pratt, R. Hough, M. Rechsteiner, J. Biol. Chem. 264, 12526 (1989); B. Bercovich et al., ibid. 272, 9002 (1997); M. Conconi et al., Biochem. J. 333, 407 (1998).
-
(1989)
J. Biol. Chem.
, vol.264
, pp. 12526
-
-
Pratt, G.1
Hough, R.2
Rechsteiner, M.3
-
33
-
-
0031004769
-
-
D. Finley, A. Ciechanover, A. Varshavsky, Cell 37, 43 (1984); G. Pratt, R. Hough, M. Rechsteiner, J. Biol. Chem. 264, 12526 (1989); B. Bercovich et al., ibid. 272, 9002 (1997); M. Conconi et al., Biochem. J. 333, 407 (1998).
-
(1997)
J. Biol. Chem.
, vol.272
, pp. 9002
-
-
Bercovich, B.1
-
34
-
-
0032527963
-
-
D. Finley, A. Ciechanover, A. Varshavsky, Cell 37, 43 (1984); G. Pratt, R. Hough, M. Rechsteiner, J. Biol. Chem. 264, 12526 (1989); B. Bercovich et al., ibid. 272, 9002 (1997); M. Conconi et al., Biochem. J. 333, 407 (1998).
-
(1998)
Biochem. J.
, vol.333
, pp. 407
-
-
Conconi, M.1
-
36
-
-
0027076820
-
-
A. G. Papavassiliou, M. Treier, C. Chavrier, D. Bohmann, Science 258, 1941 (1992).
-
(1992)
Science
, vol.258
, pp. 1941
-
-
Papavassiliou, A.G.1
Treier, M.2
Chavrier, C.3
Bohmann, D.4
-
38
-
-
0345349986
-
-
note
-
We thank R. Morimoto for heat shock reagents, M. Rechsteiner for mouse ts85 cells, G. Dreyfuss for antiserum to PABP, N. Sonenberg for antiserum to elF4G, and C. Daly, J. deDiego, and S. Srivastava for advice. Supported by NIH grants CA42357 (R.J.S.) and CA52443 (G.B.) and a fellowship from the Fundación Ramón Areces (R.C.).
-
-
-
|